Bcl-2 Antibody (8E12)

Novus Biologicals | Catalog # NBP2-22170

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, PCR

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, PCR

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8E12
View all Bcl-2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues surrounding Bcl-2, conjugated to KLH.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Bcl-2 Antibody (8E12)

Immunocytochemistry/ Immunofluorescence: Bcl-2 Antibody (8E12) - BSA Free [NBP2-22170]

Immunocytochemistry/ Immunofluorescence: Bcl-2 Antibody (8E12) - BSA Free [NBP2-22170]

Immunocytochemistry/Immunofluorescence: Bcl-2 Antibody (8E12) [NBP2-22170] - Analysis of NIH/3T3 cells using Bcl2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Bcl-2 Antibody (8E12) - BSA Free [NBP2-22170]

Immunohistochemistry-Paraffin: Bcl-2 Antibody (8E12) - BSA Free [NBP2-22170]

Immunohistochemistry-Paraffin: Bcl-2 Antibody (8E12) [NBP2-22170] - Analysis of colon cancer tissues (left) and human brain tissues (right) using Bcl2 mouse mAb with DAB staining.
Flow Cytometry: Bcl-2 Antibody (8E12) - BSA Free [NBP2-22170]

Flow Cytometry: Bcl-2 Antibody (8E12) - BSA Free [NBP2-22170]

Flow Cytometry: Bcl-2 Antibody (8E12) [NBP2-22170] - Analysis of 3T3L1 cells using Bcl2 mouse mAb (green) and negative control (purple).

Applications for Bcl-2 Antibody (8E12)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000
Application Notes
Use in Western blot reported in scientific literature (PMID: 26916241). Use in PCR reported in secitific publication PMID: 32634713

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bcl-2

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.Tissue specificity: Expressed in a variety of tissues.

Long Name

B Cell Lymphoma/Leukemia 2

Alternate Names

Bcl2

Gene Symbol

BCL2

Additional Bcl-2 Products

Product Documents for Bcl-2 Antibody (8E12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Bcl-2 Antibody (8E12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Bcl-2 Antibody (8E12)

Customer Reviews for Bcl-2 Antibody (8E12)

There are currently no reviews for this product. Be the first to review Bcl-2 Antibody (8E12) and earn rewards!

Have you used Bcl-2 Antibody (8E12)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies