Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Avian, Bovine, Chinese Hamster, Fish, Primate, Rabbit

Cited:

Human, Mouse, Rat, Porcine, Avian, Bovine, Fish, Hamster - Cricetulus (Chinese Hamster), Primate, Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Proximity Ligation Assay

Cited:

Immunohistochemistry-Paraffin, Western Blot, Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Simple Western, Proximity Ligation Assay, IF/IHC, Electron Microscopy

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all beta-Actin Primary Antibodies »

Product Specifications

Immunogen

This beta-Actin Antibody was made from a synthetic peptide made to an N-terminal region of human Beta Actin. [UniProt P60709]

Reactivity Notes

Rabbit reactivity and Fish reactivity reported in scientific literature (PMID: 23813946 and 25842206 respectively). Expected to cross-react with a wide range of species due to sequence identity. Bovine reactivity reported in scientific literature (PMID:33066332).

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit beta-Actin Antibody - BSA Free (NB600-503) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow, ICC/IF, Simple Western and IP. Anti-beta-Actin Antibody: Cited in 132 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for beta-Actin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody [NB600-503]

Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody [NB600-503]

Immunocytochemistry/Immunofluorescence: beta-Actin Antibody [NB600-503] - Cultured pig trabecular meshwork cells stained with beta-Actin antibody at a dilution of 1:500 followed by an anti-rabbit Alexa Fluor488 at 1:1000 dilution (green). Image from verified customer review.
Western Blot: beta-Actin Antibody [NB600-503]

Western Blot: beta-Actin Antibody [NB600-503]

Western Blot: beta-Actin Antibody [NB600-503] - Rabbit polyclonal at 1/5000.
Simple Western: beta-Actin Antibody [NB600-503]

Simple Western: beta-Actin Antibody [NB600-503]

Simple Western: beta-Actin Antibody [NB600-503] - Simple Western lane view shows a specific band for Beta Actin in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Flow Cytometry: beta-Actin Antibody [NB600-503]

Flow Cytometry: beta-Actin Antibody [NB600-503]

Flow Cytometry: beta-Actin Antibody [NB600-503] - An intracellular stain was performed on HeLa cells with beta-actin Antibody NB600-503AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Western Blot: beta-Actin Antibody [NB600-503]

Western Blot: beta-Actin Antibody [NB600-503]

Western Blot: beta-Actin Antibody [NB600-503] - Western blot analysis of Jurkat, COS, and CHO cell lysate using beta actin antibody at 1:100.
Immunohistochemistry-Paraffin: beta-Actin Antibody [NB600-503]

Immunohistochemistry-Paraffin: beta-Actin Antibody [NB600-503]

Immunohistochemistry-Paraffin: beta-Actin Antibody [NB600-503] - Analysis of Beta Actin in mouse epidermis using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody [NB600-503]

Immunocytochemistry/ Immunofluorescence: beta-Actin Antibody [NB600-503]

Immunocytochemistry/Immunofluorescence: beta-Actin Antibody [NB600-503] - IF Confocal analysis of HeLa cells using Beta Actin antibody (NB600-503, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). DAPI was used to stain the cell nuclei (blue).
Flow Cytometry: beta-Actin Antibody [NB600-503]

Flow Cytometry: beta-Actin Antibody [NB600-503]

Flow Cytometry: beta-Actin Antibody [NB600-503] - Analysis of HeLa cells using mouse Monoclonal beta-Actin antibody (Orange) and Isotype control Antibody (Blue).
Simple Western: beta-Actin Antibody [NB600-503]

Simple Western: beta-Actin Antibody [NB600-503]

beta-Actin-Antibody-Simple-Western-NB600-503-img0014.jpg
beta-Actin Antibody

Western Blot: beta-Actin Antibody [NB600-503] -

Western Blot: beta-Actin Antibody [NB600-503] - Regulation of inflammatory signaling in hSMC.ND (open bars) & T2D (solid bars) cells extracted after 48 hr treatment with LPS, Pioglitazone (Pio), palmitate or oleate. Results expressed relative to untreated control for each individual set of cells, Ave + SEM. (A) IkBa protein, n = 7–12 & 6–15 for ND & T2D, respectively. (B) Total & phospho-p38, n = 6–14 & 5–8. (C) Total & phospho-p44/42, n = 7–13 & 7–11. (D) Total & phospho-JNK, n = 9–12 & 3–8. * p<0.05 T2D vs ND. †p<0.05 T2D response vs ND response Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27453994), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.
beta-Actin Antibody

Western Blot: beta-Actin Antibody [NB600-503] -

Western Blot: beta-Actin Antibody [NB600-503] - Impact of T2D on inflammatory signaling in hSMC.Proteins extracted from ND (open bars) & T2D (solid bars) hSMC under control conditions. (A) Representative western blots. (B) Quantification of protein expression normalized to loading control ( beta -actin). Ave + SEM, n = 13–21 for ND, 10–16 for T2D. (C) Protein phosphorylation, expressed as ratio of phosphorylated to total protein. Ave + SEM, n = 13–17 for ND, 9–15 for T2D. *p<0.05 vs ND Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27453994), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.
beta-Actin Antibody

Western Blot: beta-Actin Antibody [NB600-503] -

Western Blot: beta-Actin Antibody [NB600-503] - Early start GM1 administration did not affect alpha -synuclein expression or transport to the striatum. (A,B) When assessed 1 week following AAV-A53T alpha -synuclein injection, levels of striatal alpha -synuclein were no different in saline (N = 6) vs. GM1-treated animals (N = 6), suggesting no influence of GM1 on A53T alpha -synuclein transduction or transport to the striatum. Representative Wes Western blots are shown after cropping (full length images of blots are presented as Supplementary Fig. 2. (C) Double label immunofluorescence 1 week after AAV-A53T alpha -synuclein injection showed no differences between saline & GM1-treated animals in alpha -synuclein accumulation (green) in TH+ neurons (red) in the SNc. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31182727), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
beta-Actin Antibody

Western Blot: beta-Actin Antibody [NB600-503] -

Western Blot: beta-Actin Antibody [NB600-503] - Anti-hnRNP A1 antibodies alter protein levels as measured by Western blot. SK-N-SH cells were cultured & treated with anti-hnRNP A1 antibodies or control IgG. Following a 48 hour incubation, cells were lysed & protein lysate was run on 10% Tris-glycine gels for Western blot analysis & probed for Beta-actin (A) or GAPDH (B)) (control), hnRNP A1, SPG4, SPG7, & SPG20. Results revealed that there was a marked reduction of SPG 4 & SPG 7 protein levels in anti-hnRNP A1 antibody compared to control isotype IgG treated cells. There was a variable response to hnRNP A1 protein & a modest reduction of SPG 20. Parentheses show relative percent reduction of signal comparing anti-hnRNP A1 antibody to control isotype IgG treatment of cells. (L=lysate, IgG=control isotype IgG, A1=anti-hnRNP A1-M9 antibody treatment of cells). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27375925), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for beta-Actin Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:10-1:500

Simple Western

1:12.5

Western Blot

1:100-1:2000
Application Notes

See Simple Western Antibody Database for Simple Western validation: tested in HeLa lysate (0.1 mg/ml); antibody dilution of 1:12.5; separated by size; detects a band at 50 kDa

Reviewed Applications

Read 4 reviews rated 5 using NB600-503 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: beta-Actin

Actin is a highly conserved protein involved in cell motility, structure, and integrity. Six functional actin isoforms exist and were named based on their electrophoretic mobility and distribution including alpha-cardiac muscle actin (aCAA), alpha-skeletal muscle actin (aSKA), alpha-smooth muscle actin (aSMA), beta-cytoplasmic actin (bCYA), gamma-cytoplasmic actin (gCYA) and gamma-smooth muscle actin (gSMA) (1). The cytoplasmic isoforms, beta-actin and gamma-actin, are nearly identical, differing by only 4 amino acids at the N-terminus with a theoretical molecular weight of 42 kDa (2). Beta-actin is ubiquitously expressed in eukaryotic cells and considered a housekeeping gene which is frequently used as a loading control for assays involving protein detection, such as Western blotting (3,4).

References

1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245

2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619

3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968

4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433

Alternate Names

ACTB, betaActin

Entrez Gene IDs

60 (Human); 11461 (Mouse); 81822 (Rat)

Gene Symbol

ACTB

UniProt

P60709

Additional beta-Actin Products

Product Documents for beta-Actin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for beta-Actin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for beta-Actin Antibody - BSA Free

Customer Reviews for beta-Actin Antibody - BSA Free (4)

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • beta-Actin Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: pig trabecular meshwork cells
    Species: Pig
    Verified Customer | Posted 12/05/2016
    Detection of Actin in cultured pig trabecular meshwork cells using beta-Actin Antibody (NB600-503) at 1:500 dilution overnight and donkey anti-rabbit Alexa 488 (1:1000) for 1 hour.
    beta-Actin Antibody - BSA Free NB600-503
  • Name: Bryan Tinsley
    Application: Immunocytochemistry
    Sample Tested:
    Species: Human
    Verified Customer | Posted 06/11/2014
    IF Confocal analysis of HeLa cells using Beta Actin antibody (NB600-503, 1:5).
    beta-Actin Antibody - BSA Free NB600-503
  • Name: Bryan Tinsley
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 06/11/2014
    Western blot analysis of extracts from Jurkat, COS and CHO cells using Actin antibody (NB600-503, 1:200).
    beta-Actin Antibody - BSA Free NB600-503
  • Name: Astrid Spannhoff
    Application: Western Blot
    Sample Tested: NIH 3T3 cell lysate, Sample Amount: 5 ul
    Species: Mouse
    Verified Customer | Posted 11/19/2010
    beta-Actin Antibody - BSA Free NB600-503

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Protocols

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FAQs for beta-Actin Antibody - BSA Free

Showing  1 - 5 of 6 FAQs Showing All

    • A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.

    • A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.

    • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
      A: For homogenized tissue, beta-actin and GAPDH are both fine.

    • A: A sequence within the N-terminal region of Human beta Actin. The exact sequence is proprietary.

    • A: The TMW for beta Actin antibodies is approximately 42 kDa.

    • A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
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