Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Amphibian

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Electron Microscopy

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Electron Microscopy

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all beta-Actin Primary Antibodies »

Product Specifications

Immunogen

beta-Actin Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near amino acids 350-375 of Human beta Actin. (Uniprot: P60709)

Reactivity Notes

Lithobates (Leopard frog)

beta-Actin Loading Control Antibody is expected to cross-react with a wide range of species due to sequence homology. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for beta-Actin Antibody - BSA Free

Western Blot: beta-Actin Antibody [NB600-505]

Western Blot: beta-Actin Antibody [NB600-505]

beta-Actin-Antibody-Western-Blot-NB600-505-img0008.jpg
Immunohistochemistry: beta-Actin Antibody [NB600-505]

Immunohistochemistry: beta-Actin Antibody [NB600-505]

Immunohistochemistry: beta-Actin Antibody [NB600-505] - Staining at 1:200 shows detection of actin at the neuromuscular juntion of rana pipiens tissue. Formalin fixed paraffin embedded sections.
Western Blot: beta-Actin Antibody [NB600-505]

Western Blot: beta-Actin Antibody [NB600-505]

Western Blot: beta-Actin Antibody [NB600-505] - Detection of actin Lane 1: HeLa cell lysate, Lane 2: rat brain, Lane 3: mouse brain, Lane 4: 3T3 cell lysate.
Western Blot: beta-Actin Antibody [NB600-505]

Western Blot: beta-Actin Antibody [NB600-505]

Western Blot: beta-Actin Antibody [NB600-505] - analysis of Beta Actin on HeLa and LNCaP cell lysates(25ug/lane) using anti-Beta Actin antibody. Image from verified customer review.
Western Blot: beta-Actin Antibody [NB600-505]

Western Blot: beta-Actin Antibody [NB600-505]

Western Blot: beta-Actin Antibody [NB600-505] - Lane 1: molecular weight. Lane 2: human embryonic kidney 293. Lane 3: human lung carcinoma A549. Lane 4: mouse brain. Load: 35 ug per lane. Primary antibody: Beta Actin antibody at 1:1,500 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 42 kDa corresponding to beta Actin (arrowhead). Other band(s): none.
beta-Actin Antibody

beta-Actin Antibody

Immunohistochemistry of Rabbit beta-Actin Antibody. Tissue: sections 4.2 mm thick of rana pipiens tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Beta Actin antibody at 1:200 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Beta Actin is at the neuromuscular junction. Staining: Beta Actin as precipitated green signal with red and blue counterstain.
beta-Actin Antibody

Western Blot: beta-Actin Antibody [NB600-505] -

(A) Representative western blot bands: MMP-9, MMP-12 and beta -actin in lung homogenates of the CS and Air groups from different gels. Full-length gels and blots are shown in a supplementary information (Supplemental Fig. 2) (B,C) Expression of MMP-9 (B) and MMP-12 (C) in the CS group compared with the Air group after normalization to beta -actin. (D) Total cells per 1 ml bronchoalveolar lavage fluid. (E) The ratio of neutrophil in bronchoalveolar lavage fluid. *p < 0.05 vs corresponding Air groups.
beta-Actin Antibody

Western Blot: beta-Actin Antibody [NB600-505] -

Western Blot: beta-Actin Antibody [NB600-505] - SUM149PT cells express a truncated partially functional BRCA1 protein.Cells were irradiated with 6 Gy & lysed after 6 hours for analysis by Western blotting. Data for three independent experiments are shown (Exp 1–3). Note the band shifting after irradiation probably reflecting changes in the phosphorylation status. Mr, molecular weight marker; BRCA1-FL, full-length BRCA1 protein; BRCA1-delta Ex11, truncated BRCA1 protein lacking exon 11. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep28217), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
beta-Actin Antibody

Western Blot: beta-Actin Antibody [NB600-505] -

Western Blot: beta-Actin Antibody [NB600-505] - BRCA1-mutant cell lines show distinct sensitivities to 6-thoguanine.(a) Sensitivity of BRCA1-mutant (red) & BRCA1-wild-type (blue) cell lines to 6-thioguanine was tested in 96-well plates in four replicas using CellTiter-Blue metabolic assay. Each data point represents an average of four replicas. Non-linear regression curves were calculated using GraphPad Prism. (b) Western blot demonstrating that BRCA1-mutant (red) cell lines express full-length 53BP1 protein, but not BRCA1. (c) Quantification of 53BP1 protein expression for select cell lines. Individual normalized band intensity values in arbitrary units (A.U.) from three independent experiments are shown. Red lines show average values for each cell line. Statistical significance was evaluated with the Dunn’s multiple comparisons test following a significant non-parametric Kruskal-Wallis test using GraphPad Prism software. *p < 0,05. (d) Western blot reveals expression of mismatch repair proteins MSH2 & MLH1 in all cells, but lack of the HPRT protein in SUM1315MO2 cell line, likely explaining its resistance to 6-thioguanine. beta -actin is used as loading control. (e) Quantification of HPRT protein expression for select cell lines as described in (c). Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep28217), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
beta-Actin Antibody

Western Blot: beta-Actin Antibody [NB600-505] -

Western Blot: beta-Actin Antibody [NB600-505] - BRCA1-mutant cell lines show distinct sensitivities to 6-thoguanine.(a) Sensitivity of BRCA1-mutant (red) & BRCA1-wild-type (blue) cell lines to 6-thioguanine was tested in 96-well plates in four replicas using CellTiter-Blue metabolic assay. Each data point represents an average of four replicas. Non-linear regression curves were calculated using GraphPad Prism. (b) Western blot demonstrating that BRCA1-mutant (red) cell lines express full-length 53BP1 protein, but not BRCA1. (c) Quantification of 53BP1 protein expression for select cell lines. Individual normalized band intensity values in arbitrary units (A.U.) from three independent experiments are shown. Red lines show average values for each cell line. Statistical significance was evaluated with the Dunn’s multiple comparisons test following a significant non-parametric Kruskal-Wallis test using GraphPad Prism software. *p < 0,05. (d) Western blot reveals expression of mismatch repair proteins MSH2 & MLH1 in all cells, but lack of the HPRT protein in SUM1315MO2 cell line, likely explaining its resistance to 6-thioguanine. beta -actin is used as loading control. (e) Quantification of HPRT protein expression for select cell lines as described in (c). Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep28217), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for beta-Actin Antibody - BSA Free

Application
Recommended Usage

ELISA

1:10000-1:40000

Immunocytochemistry/ Immunofluorescence

1:500-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:1000-1:4000
Application Notes
This product has been tested for use in ELISA, immunofluorescence, and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.

Use in electron microscopy reported in scientific literature (PMID: 18799754).

Reviewed Applications

Read 1 review rated 5 using NB600-505 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta-Actin

Actin is a highly conserved protein involved in cell motility, structure, and integrity. Six functional actin isoforms exist and were named based on their electrophoretic mobility and distribution including alpha-cardiac muscle actin (aCAA), alpha-skeletal muscle actin (aSKA), alpha-smooth muscle actin (aSMA), beta-cytoplasmic actin (bCYA), gamma-cytoplasmic actin (gCYA) and gamma-smooth muscle actin (gSMA) (1). The cytoplasmic isoforms, beta-actin and gamma-actin, are nearly identical, differing by only 4 amino acids at the N-terminus with a theoretical molecular weight of 42 kDa (2). Beta-actin is ubiquitously expressed in eukaryotic cells and considered a housekeeping gene which is frequently used as a loading control for assays involving protein detection, such as Western blotting (3,4).

References

1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245

2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619

3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968

4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433

Alternate Names

ACTB, betaActin

Entrez Gene IDs

60 (Human); 11461 (Mouse)

Gene Symbol

ACTB

UniProt

P60709

Additional beta-Actin Products

Product Documents for beta-Actin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for beta-Actin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for beta-Actin Antibody - BSA Free

Customer Reviews for beta-Actin Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Hela and LNCap cell lysates
    Species: Human
    Verified Customer | Posted 10/07/2014
    WB for actin antibody
    beta-Actin Antibody - BSA Free NB600-505

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Protocols

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FAQs for beta-Actin Antibody - BSA Free

Showing  1 - 5 of 6 FAQs Showing All

  • Q: Are the beta-Actin antibodies validated in Simple Western?

    A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.

  • Q: Do Beta-Actin antibodies come in lyophilized format?

    A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: What is the immunogen sequence of this Beta-Actin antibody?

    A: A sequence corresponding to amino acids 359 - 368 of beta Actin.

  • Q: What is the theoretical molecular weight for Beta-Actin antibodies?

    A: The TMW for beta Actin antibodies is approximately 42 kDa.

  • Q: Why is beta actin used as a loading control in blotting ?

    A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.

  • Q: Are the beta-Actin antibodies validated in Simple Western?

    A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.

  • Q: Do Beta-Actin antibodies come in lyophilized format?

    A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: What is the immunogen sequence of this Beta-Actin antibody?

    A: A sequence corresponding to amino acids 359 - 368 of beta Actin.

  • Q: What is the theoretical molecular weight for Beta-Actin antibodies?

    A: The TMW for beta Actin antibodies is approximately 42 kDa.

  • Q: Why is beta actin used as a loading control in blotting ?

    A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.

  • Q: Are the beta-Actin antibodies validated in Simple Western?

    A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.

  • Q: Do Beta-Actin antibodies come in lyophilized format?

    A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: What is the immunogen sequence of this Beta-Actin antibody?

    A: A sequence corresponding to amino acids 359 - 368 of beta Actin.

  • Q: What is the theoretical molecular weight for Beta-Actin antibodies?

    A: The TMW for beta Actin antibodies is approximately 42 kDa.

  • Q: Why is beta actin used as a loading control in blotting ?

    A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.

  • Q: Are the beta-Actin antibodies validated in Simple Western?

    A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.

  • Q: Do Beta-Actin antibodies come in lyophilized format?

    A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: What is the immunogen sequence of this Beta-Actin antibody?

    A: A sequence corresponding to amino acids 359 - 368 of beta Actin.

  • Q: What is the theoretical molecular weight for Beta-Actin antibodies?

    A: The TMW for beta Actin antibodies is approximately 42 kDa.

  • Q: Why is beta actin used as a loading control in blotting ?

    A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.

  • Q: Are the beta-Actin antibodies validated in Simple Western?

    A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.

  • Q: Do Beta-Actin antibodies come in lyophilized format?

    A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: What is the immunogen sequence of this Beta-Actin antibody?

    A: A sequence corresponding to amino acids 359 - 368 of beta Actin.

  • Q: What is the theoretical molecular weight for Beta-Actin antibodies?

    A: The TMW for beta Actin antibodies is approximately 42 kDa.

  • Q: Why is beta actin used as a loading control in blotting ?

    A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.

  • Q: Are the beta-Actin antibodies validated in Simple Western?

    A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.

  • Q: Do Beta-Actin antibodies come in lyophilized format?

    A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: What is the immunogen sequence of this Beta-Actin antibody?

    A: A sequence corresponding to amino acids 359 - 368 of beta Actin.

  • Q: What is the theoretical molecular weight for Beta-Actin antibodies?

    A: The TMW for beta Actin antibodies is approximately 42 kDa.

  • Q: Why is beta actin used as a loading control in blotting ?

    A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.

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