beta-Catenin Antibody (1C9) - Azide and BSA Free

Name: beta-Catenin Antibody (1C9) - Azide and BSA Free
Brand: Novus Biologicals
SKU: H00001499-M02
Price: 529 USD
Availability: InStock

Novus Biologicals | Catalog # H00001499-M02

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1C9

Format

Azide and BSA Free

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Product Specifications

Immunogen

CTNNB1 (AAH58926, 682 a.a. ~ 781 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL

Marker

Epithelial Cell Marker, Adherens Junction Marker

Specificity

CTNNB1 (1C9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for beta-Catenin Antibody (1C9) - Azide and BSA Free

Western Blot: beta-Catenin Antibody (1C9) [H00001499-M02]

Western Blot: beta-Catenin Antibody (1C9) [H00001499-M02] - Analysis of CTNNB1 expression in transfected 293T cell line by CTNNB1 monoclonal antibody (M02), clone 1C9.Lane 1: CTNNB1 transfected lysate(85.5 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (1C9) [H00001499-M02]

Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (1C9) [H00001499-M02] - PC-3 MM2 cells were stained with CTNNB1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).

Western Blot: beta-Catenin Antibody (1C9) [H00001499-M02]

Western Blot: beta-Catenin Antibody (1C9) [H00001499-M02] - Analysis of CTNNB1 over-expressed 293 cell line, cotransfected with CTNNB1 Validated Chimera RNAi ( Cat # H00001499-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CTNNB1 monoclonal antibody (M02), clone 1C9 (Cat # H00001499-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (1C9) [H00001499-M02]

Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (1C9) [H00001499-M02] - Analysis of monoclonal antibody to CTNNB1 on HeLa cell. Antibody concentration 10 ug/ml.

Proximity Ligation Assay: beta-Catenin Antibody (1C9) [H00001499-M02]

Proximity Ligation Assay: beta-Catenin Antibody (1C9) [H00001499-M02] - Analysis of protein-protein interactions between GSK3B and CTNNB1. HeLa cells were stained with anti-GSK3B rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Proximity Ligation Assay: beta-Catenin Antibody (1C9) [H00001499-M02]

Proximity Ligation Assay: beta-Catenin Antibody (1C9) [H00001499-M02] - Analysis of protein-protein interactions between FLT1 and CTNNB1. Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

ELISA: beta-Catenin Antibody (1C9) [H00001499-M02]

ELISA: beta-Catenin Antibody (1C9) [H00001499-M02] - Detection limit for recombinant GST tagged CTNNB1 is 0.1 ng/ml as a capture antibody.

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Applications for beta-Catenin Antibody (1C9) - Azide and BSA Free

Application

Recommended Usage

Western Blot

1:500

Application Notes

Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA and RNAi Validation.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: beta-Catenin

Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]

Alternate Names

bCatenin, CTNNB1

Entrez Gene IDs

1499 (Human)

Gene Symbol

CTNNB1

OMIM

114550 (Human)

UniProt

AAH58926 (Human)

Additional beta-Catenin Products

Product Documents for beta-Catenin Antibody (1C9) - Azide and BSA Free

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Product Specific Notices for beta-Catenin Antibody (1C9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.