beta-Catenin Antibody (CL3689) - Azide and BSA Free

Name: beta-Catenin Antibody (CL3689) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-44056
Price: 589 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-44056

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # CL3689

Format

Azide and BSA Free

View all beta-Catenin Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P35222, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for beta-Catenin Antibody (CL3689) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP3-44056]

Staining of A-431 cells using the Anti-beta-Catenin monoclonal antibody).

Western Blot: beta-Catenin Antibody [NBP3-44056]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line SK-MEL-30

Western Blot: beta-Catenin Antibody [NBP3-44056]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line A-549

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP3-44056]

Staining of human breast cancer shows membranous immunoreactivity in tumor cells.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP3-44056]

Staining of human duodenum shows strong membranous immunoreactivity in epithelial cells.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP3-44056]

Staining of human cervix shows strong membranous positivity in epithelial cells.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP3-44056]

Staining of human kidney shows strong membranous positivity in renal tubules and glomerulus cells.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP3-44056]

Staining of human endometrium shows strong membranous immunoreactivity in glandular epithelium.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP3-44056]

Staining of human renal cancer shows membranous positivity in tumor cells.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP3-44056]

Staining of human prostate cancer shows membranous positivity in tumor cells.

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP3-44056]

Staining of human skeletal muscle shows absence of immunoreactivity in muscle fibers (negative control).

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP3-44056]

Staining of U-251 cells using the Anti-beta-Catenin monoclonal antibody).

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Applications for beta-Catenin Antibody (CL3689) - Azide and BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:20000 - 1:50000

Western Blot

1 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: beta-Catenin

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

bCatenin, CTNNB1

Gene Symbol

CTNNB1

Additional beta-Catenin Products

Product Documents for beta-Catenin Antibody (CL3689) - Azide and BSA Free

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Product Specific Notices for beta-Catenin Antibody (CL3689) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.