beta-Catenin Antibody (CTNNB1/2030R)

Name: beta-Catenin Antibody (CTNNB1/2030R)
Brand: Novus Biologicals
SKU: NBP2-79753
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-79753

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # CTNNB1/2030R

View all beta-Catenin Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human beta-Catenin protein (Uniprot: P35222 )

Localization

Cell Surface, Cytoplasm

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified by Protein A Column. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79888)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for beta-Catenin Antibody (CTNNB1/2030R)

Western Blot: beta-Catenin Antibody (CTNNB1/2030R) [NBP2-79753]

Western Blot: beta-Catenin Antibody (CTNNB1/2030R) [NBP2-79753] - Western Blot Analysis of HeLa cell lysate using beta-Catenin Antibody (CTNNB1/2030R).

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (CTNNB1/2030R) [NBP2-79753]

Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (CTNNB1/2030R) [NBP2-79753] - Immunofluorescence Analysis of HeLa cells labeling Beta-Catenin with beta-Catenin Antibody (CTNNB1/2030R) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red)

Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/2030R) [NBP2-79753]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/2030R) [NBP2-79753] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with beta-Catenin Antibody (CTNNB1/2030R).

Flow Cytometry: beta-Catenin Antibody (CTNNB1/2030R) [NBP2-79753]

Flow Cytometry: beta-Catenin Antibody (CTNNB1/2030R) [NBP2-79753] - Flow Cytometric Analysis of HeLa cells using beta-Catenin Antibody (CTNNB1/2030R)followed by Goat anti-Rabbit IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/2030R) [NBP2-79753]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/2030R) [NBP2-79753] - Formalin-fixed, paraffin-embedded human Tonsil stained with beta-Catenin Antibody (CTNNB1/2030R).

Protein Array: beta-Catenin Antibody (CTNNB1/2030R) [NBP2-79753]

Protein Array: beta-Catenin Antibody (CTNNB1/2030R) [NBP2-79753] - Analysis of Protein Array containing more than 19,000 full-length human proteins using beta-Catenin Antibody (CTNNB1/2030R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

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Applications for beta-Catenin Antibody (CTNNB1/2030R)

Application

Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml

Application Notes

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Advanced Features

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Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: beta-Catenin

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

bCatenin, CTNNB1

Gene Symbol

CTNNB1

UniProt

P35222

Additional beta-Catenin Products

Product Documents for beta-Catenin Antibody (CTNNB1/2030R)

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Certificate of Analysis

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Product Specific Notices for beta-Catenin Antibody (CTNNB1/2030R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.