beta-Catenin Antibody (OTI12H7)

Name: beta-Catenin Antibody (OTI12H7)
Brand: Novus Biologicals
SKU: NBP2-03615
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-03615

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # OTI12H7

View all beta-Catenin Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CTNNB1 (NP_001895) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Marker

Epithelial Cell Marker, Adherens Junction Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

85.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta-Catenin Antibody (OTI12H7)

Western Blot: beta-Catenin Antibody (OTI12H7) [NBP2-03615]

Western Blot: beta-Catenin Antibody (12H7) [NBP2-03615] - Analysis of extracts (35ug) from 9 different cell lines by using anti-beta Catenin monoclonal antibody.

Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (OTI12H7) [NBP2-03615]

Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY beta Catenin.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (OTI12H7) [NBP2-03615]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Human prostate tissue using anti-beta Catenin mouse monoclonal antibody.

Flow Cytometry: beta-Catenin Antibody (OTI12H7) [NBP2-03615]

Flow Cytometry: beta-Catenin Antibody (12H7) [NBP2-03615] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-beta Catenin antibody, and then analyzed by flow cytometry.

Western Blot: beta-Catenin Antibody (OTI12H7) [NBP2-03615]

Western Blot: beta-Catenin Antibody (12H7) [NBP2-03615] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta Catenin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-beta Catenin.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (OTI12H7) [NBP2-03615]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-beta Catenin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (OTI12H7) [NBP2-03615]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-beta Catenin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (OTI12H7) [NBP2-03615]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-beta Catenin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (OTI12H7) [NBP2-03615]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-beta Catenin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (OTI12H7) [NBP2-03615]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Human endometrium tissue using anti-beta Catenin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: beta-Catenin Antibody (OTI12H7) [NBP2-03615]

Immunohistochemistry-Paraffin: beta-Catenin Antibody (12H7) [NBP2-03615] - Staining of paraffin-embedded Human pancreas tissue using anti-beta Catenin mouse monoclonal antibody.

Flow Cytometry: beta-Catenin Antibody (OTI12H7) [NBP2-03615]

Flow Cytometry: beta-Catenin Antibody (12H7) [NBP2-03615] - Analysis of Hela cells, using anti-beta Catenin antibody, (Red), compared to a nonspecific negative control antibody (Blue).

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Applications for beta-Catenin Antibody (OTI12H7)

Application

Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:1000-2000

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta-Catenin

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternate Names

bCatenin, CTNNB1

Entrez Gene IDs

1499 (Human)

Gene Symbol

CTNNB1

Additional beta-Catenin Products

Product Documents for beta-Catenin Antibody (OTI12H7)

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Certificate of Analysis

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Product Specific Notices for beta-Catenin Antibody (OTI12H7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.