Bim Antibody - BSA Free

Western Blot: Bim AntibodyBSA Free [NBP1-76963]

Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Human Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: Bim Antibody - BSA Free [NBP1-76963] -

Western Blot: Bim Antibody - BSA Free [NBP1-76963] - Figure 3 Western Blot Validation in Human TissueLoading: 15 u of lysates per lane.Antibodies: BIM, (0.5 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human thymusLane 2: Human colon

Western Blot: Bim Antibody - BSA Free [NBP1-76963] -

EGb761 regulates Bcl-2 family proteins expression in melanoma cells.(A) EGb761 alters the expression levels of anti- & pro-apoptotic Bcl-2 family proteins in melanoma cell lines. Whole cell lysates from Mel-RM & Mel-AT cells treated with EGb761 (400 μg/ml) for indicated time periods were subjected to Western blot analysis. The data shown are representative of three individual experiments. (B) 5% ethanol as control vehicle did not alter the expression levels of Mcl-1. Mel-AT cells with 5% ethanol for increasing periods. Whole cell lysates from Mel-AT cells treated were subjected to Western blot analysis. The data shown are representative of three individual experiments.(C) Mel-RM & Mel-AT cells were treated with EGb761 (400 μg/ml) or 5% ethanol for the indicated periods. Total RNA was isolated & subjected to real-time PCR analysis for Mcl-1. The relative abundance of mRNA expression treated with 5% ethanol was arbitrarily designated as 1. Columns, mean of three individual experiments; bars, SEM. * Present p<0.05 vs control.(D) Relative expression of anti-apoptosis Bcl-2 family proteins in melanoma cell lines Mel-RM & Mel-AT without treatment. Quantitative expression levels of Mcl-1, Bcl-2 & Bcl-XL were normalized to GAPDH.(E) Relative expression of pro-apoptosis Bcl-2 family proteins in melanoma cell lines Mel-RM & Mel-AT without treatment. Quantitative expression levels of Bax, Bid, Noxa, PUMA, Bim, Bad & Bak were normalized to GAPDH. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25860257), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Bim Antibody - BSA Free [NBP1-76963] -

Immunohistochemistry: Bim Antibody - BSA Free [NBP1-76963] - Figure 7 Immunohistochemistry Validation of BIM in Human Skin Cancer Cells
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-BIM antibody at 20 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-76963] -

Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-76963] - Validation of BIM in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Bim with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).