BMI-1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-87026

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Chicken (100%), Feline (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BMI-1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 276 and 326 of human B lymphoma Mo-MLV insertion region 1 homolog using the numbering given in entry NP_005171.4 (GeneID 648).

Reactivity Notes

Cat (100%), Orangutan (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BMI-1 Antibody - BSA Free

Western Blot: BMI-1 Antibody [NB100-87026]

Western Blot: BMI-1 Antibody [NB100-87026]

Western Blot: BMI-1 Antibody [NB100-87026] - Detection of human BMI1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, LNCaP, RKO, Hep-G2, U2OS, A-549, Jurkat, K-562, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-RPS3.
Immunohistochemistry-Paraffin: BMI-1 Antibody [NB100-87026]

Immunohistochemistry-Paraffin: BMI-1 Antibody [NB100-87026]

Immunohistochemistry-Paraffin: BMI-1 Antibody [NB100-87026] - Human pancreatic islet cell carcinoma (left) and mouse renal cell carcinoma (right). Antibody used at a dilution of 1:1000 (1ug/ml).
Western Blot: BMI-1 Antibody [NB100-87026]

Western Blot: BMI-1 Antibody [NB100-87026]

Western Blot: BMI-1 Antibody [NB100-87026] - Detection of Human and Mouse BMI1 on HeLa whole cell lysate using NB100-87026. BMI1 was also immunoprecipitated by another rabbit anti-BMI1 antibody.
Western Blot: BMI-1 Antibody [NB100-87026]

Western Blot: BMI-1 Antibody [NB100-87026]

Western Blot: BMI-1 Antibody [NB100-87026] - Detection of Human and Mouse BMI1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: BMI-1 Antibody [NB100-87026]

Western Blot: BMI-1 Antibody [NB100-87026]

Western Blot: BMI-1 Antibody [NB100-87026] - Detection of mouse BMI1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-BMI1 antibody NB100-87026 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-RPS3.
Immunoprecipitation: BMI-1 Antibody [NB100-87026]

Immunoprecipitation: BMI-1 Antibody [NB100-87026]

Immunoprecipitation: BMI-1 Antibody [NB100-87026] - Detection of human BMI1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BMI1 antibody NB100-87026 used for IP at 6 ug/mg lysate. BMI1 was also immunoprecipitated by another rabbit anti-BMI1 antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for BMI-1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 4 using NB100-87026 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BMI-1

BMI1 (B lymphoma Mo-MLV insertion region 1 homolog) is a core component of the polycomb group (PcG) multiprotein PRC1 complex which plays an important role in transcriptional repression of gene expression during development. BMI1 association with RING protein 1B is important to the E3 ligase activity of RING1B in the PRC1 complex. BMI1 is an oncogene whose overexpression is associated with many types of human tumors and is a stem-cell marker and regulator of stem cell self-renewal. Alternate names for BMI1 include polycomb complex protein BMI-1, polycomb group RING finger protein 4, RING finger protein 51, PCGF4, and RNF51.

Long Name

B Lymphoma Mo-MLV Insertion Region 1

Alternate Names

BMI1, PCGF4, RNF51

Entrez Gene IDs

648 (Human); 12151 (Mouse)

Gene Symbol

BMI1

UniProt

P35226

Additional BMI-1 Products

Product Documents for BMI-1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BMI-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BMI-1 Antibody - BSA Free

Customer Reviews for BMI-1 Antibody - BSA Free (1)

4 out of 5
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: fetal lung fibroblast HFL1, Sample Amount: 40 ug
    Species: Other
    Verified Customer | Posted 07/29/2009

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