BMP-2 Antibody (9E10G12) - BSA Free

Novus Biologicals | Catalog # NBP2-52422

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 9E10G12

Format

BSA Free
View all BMP-2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human BMP-2 (AA: 283-396) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

44.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BMP-2 Antibody (9E10G12) - BSA Free

Western Blot: BMP-2 Antibody (9E10G12)BSA Free [NBP2-52422]

Western Blot: BMP-2 Antibody (9E10G12)BSA Free [NBP2-52422]

Western Blot: BMP-2 Antibody (9E10G12) [NBP2-52422] - Western blot analysis using BMP2 mAb against human BMP2 (AA: 283-396) recombinant protein. (Expected MW is 38.7 kDa)
Immunocytochemistry/ Immunofluorescence: BMP-2 Antibody (9E10G12) - BSA Free [NBP2-52422]

Immunocytochemistry/ Immunofluorescence: BMP-2 Antibody (9E10G12) - BSA Free [NBP2-52422]

Immunocytochemistry/Immunofluorescence: BMP-2 Antibody (9E10G12) [NBP2-52422] - Analysis of MCF-7 cells using BMP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow Cytometry: BMP-2 Antibody (9E10G12) - BSA Free [NBP2-52422]

Flow Cytometry: BMP-2 Antibody (9E10G12) - BSA Free [NBP2-52422]

Flow Cytometry: BMP-2 Antibody (9E10G12) [NBP2-52422] - Analysis of Hela cells using BMP2 mouse mAb (green) and negative control (red).
Western Blot: BMP-2 Antibody (9E10G12)BSA Free [NBP2-52422]

Western Blot: BMP-2 Antibody (9E10G12)BSA Free [NBP2-52422]

Western Blot: BMP-2 Antibody (9E10G12) [NBP2-52422] - Analysis using BMP2 mAb against HEK293 (1) and BMP2 (AA: 283-396)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: BMP-2 Antibody (9E10G12) - BSA Free [NBP2-52422]

Immunocytochemistry/ Immunofluorescence: BMP-2 Antibody (9E10G12) - BSA Free [NBP2-52422]

Immunocytochemistry/Immunofluorescence: BMP-2 Antibody (9E10G12) [NBP2-52422] - Immunofluorescence analysis of Hela cells using BMP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
ELISA: BMP-2 Antibody (9E10G12) - BSA Free [NBP2-52422]

ELISA: BMP-2 Antibody (9E10G12) - BSA Free [NBP2-52422]

ELISA: BMP-2 Antibody (9E10G12) [NBP2-52422] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Applications for BMP-2 Antibody (9E10G12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BMP-2

Bone morphogenic proteins (BMPs) are members of the TGFbeta superfamily. BMPs are involved in the induction of cartilage and bone formation. In vivo studies have shown that BMP-2 (also designated BMP-2A) and BMP-3 can independently induce cartilage formation. Smad3 association with the TGFbeta receptor complex and Smad1 translocation to the nucleus are observed after the addition of BMP-4 (also designated BMP-2B), suggesting that BMP-4 may play a role in activation of the Smad pathway. BMP-5, BMP-6 and BMP-7 all share high sequence homology with BMP-2, indicating that they each may be able to induce cartilage formation. BMP-8 (also designated OP-2) is thought to be involved in early development, as detectable expression has not been found in adult organs.

Long Name

Bone Morphogenetic Protein 2

Alternate Names

BDA2, BMP2, SSFSC

Gene Symbol

BMP2

Additional BMP-2 Products

Product Documents for BMP-2 Antibody (9E10G12) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for BMP-2 Antibody (9E10G12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for BMP-2 Antibody (9E10G12) - BSA Free

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  • Q: I am interested in Bone Morphogenic Protein 2 (BMP-2) for clinical trial in humans. I am using this protein in stem cells culture for future use in humans. Do you have BMP-2 in pharma grade that I can use for humans?

    A: Our antibodies are for research purposes only unfortunately. I am sorry we cannot be of further help.

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