Amino Acid Sequence: MSPKHHSQRA RKKNKNCRRH SLYVDFSDVG WNDWIVAPPG YQAFYCHGDC PFPLADHLNS TNHAIVQTLV NSVNSSIPKA CCVPTELSAI SMLYLDEYDK VVLKNYQEMV VEGCGCR

Purity

>95%, by SDS-PAGE

Endotoxin Level

Less than 1 EU/ug of BMP-4 as determined by LAL method.

Predicted Molecular Mass

13.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

1. Molecular standard (Western, ELISA) in studying secreted BMP-4;
2. Preparing antibodies for BMP-4 monomer;
3. Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human BMP-4 Protein

SDS-PAGE: Recombinant Human BMP-4 Protein [NBP2-34931]

SDS-Page: Human BMP-4 Protein [NBP2-34931]

Formulation, Preparation, and Storage

NBP2-34931

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 50 mM Na2CO3, 5 mM DTT, 0.02% Tween 20, pH 11.0.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water to a concentration of 0.1-1.0mg/mL
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: BMP-4

BMP-4 plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair. Mature BMP-4 forms homodimers and heterodimers with BMP-7. BMP-4/BMP-7 heterodimers show greater potency at osteogenic differentiation compared to BMP-4 homodimers. The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans.

Long Name

Bone Morphogenetic Protein 4

Alternate Names

BMP2B, BMP2B1, BMP4, MCOPS6, OFC11

Gene Symbol

BMP4

UniProt

P12644

Additional BMP-4 Products

Product Documents for Recombinant Human BMP-4 Protein

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Recombinant Human BMP-4 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.