BRCA1 Antibody (17F8) - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-41185

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Knockout Validated, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 17F8

Format

Azide and BSA Free
View all BRCA1 Primary Antibodies »

Product Specifications

Immunogen

Protein fragment expressed in E. coli corresponding to amino acids 762-1315.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Nuclear/Cytoplasmic

Specificity

This antibody does not recognize the delta exon 11 splice variant of BRCA1. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

208 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BRCA1 Antibody (17F8) - Azide and BSA Free

Western Blot: BRCA1 Antibody (17F8) [NBP1-41185]

Western Blot: BRCA1 Antibody (17F8) [NBP1-41185]

Western Blot: BRCA1 Antibody (17F8) [NBP1-41185] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade.
Chromatin Immunoprecipitation: BRCA1 Antibody (17F8) [NBP1-41185] - ChIP grade BRCA1 antibody 6B4 and BRCA1 antibody 17F8 were used for ChIP assay. The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay. Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.
Western Blot: BRCA1 Antibody (17F8) [NBP1-41185]

Western Blot: BRCA1 Antibody (17F8) [NBP1-41185]

Western Blot: BRCA1 Antibody (17F8) [NBP1-41185] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 5 % SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade BRCA1 diluted by 1:500
Immunoprecipitation: BRCA1 Antibody (17F8) [NBP1-41185]

Immunoprecipitation: BRCA1 Antibody (17F8) [NBP1-41185]

Immunoprecipitation: BRCA1 Antibody (17F8) [NBP1-41185] - BRCA1 antibody 6B4 and BRCA1 antibody 17F8 was used for IP-WB assay. 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract. Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution. HeLa whole cell extract (20 microgram) was used as input in the Western blot assay.
BRCA1 Antibody (17F8)

Western Blot: BRCA1 Antibody (17F8) [NBP1-41185] -

Western Blot: BRCA1 Antibody (17F8) [NBP1-41185] - Wild-type (WT) and BRCA1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for BRCA1 Antibody (17F8) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Assay dependent

ELISA

Assay dependent

Immunocytochemistry/ Immunofluorescence

1 ug/ml

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Immunoprecipitation

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BRCA1

BRCA1-17F8 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the ?exon 11 splice variant. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.

Long Name

Breast Cancer 1

Alternate Names

BRCAI, breast and ovarian cancer susceptibility protein 1, breast and ovarian cancer sususceptibility protein, breast cancer 1, early onset, breast cancer type 1 susceptibility protein, EC 6.3.2, EC 6.3.2.-, IRIS, PNCA4, PSCP, subunit 1

Entrez Gene IDs

672 (Human)

Gene Symbol

BRCA1

Additional BRCA1 Products

Product Documents for BRCA1 Antibody (17F8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for BRCA1 Antibody (17F8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BRCA1 Antibody (17F8) - Azide and BSA Free

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Protocols

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