BRD4 Antibody (CL1115) - BSA Free

Novus Biologicals | Catalog # NBP2-52958

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1115

Format

BSA Free
View all BRD4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from O60885, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for BRD4 Antibody (CL1115) - BSA Free

Western Blot: BRD4 Antibody (CL1115) [NBP2-52958]

Western Blot: BRD4 Antibody (CL1115) [NBP2-52958]

Western Blot: BRD4 Antibody (CL1115) [NBP2-52958] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-BRD4 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: BRD4 Antibody (CL1115) [NBP2-52958]

Immunocytochemistry/ Immunofluorescence: BRD4 Antibody (CL1115) [NBP2-52958]

Immunocytochemistry/Immunofluorescence: BRD4 Antibody (CL1115) [NBP2-52958] - Staining of MCF-7 cells using the Anti-BRD4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: BRD4 Antibody (CL1115) [NBP2-52958]

Immunohistochemistry-Paraffin: BRD4 Antibody (CL1115) [NBP2-52958]

Immunohistochemistry-Paraffin: BRD4 Antibody (CL1115) [NBP2-52958] - Staining of human kidney shows moderate nuclear positivity in cells in renal glomeruli and in tubules.
Immunohistochemistry-Paraffin: BRD4 Antibody (CL1115) [NBP2-52958]

Immunohistochemistry-Paraffin: BRD4 Antibody (CL1115) [NBP2-52958]

Immunohistochemistry-Paraffin: BRD4 Antibody (CL1115) [NBP2-52958] - Staining of human stomach cancer shows moderate nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: BRD4 Antibody (CL1115) [NBP2-52958]

Immunohistochemistry-Paraffin: BRD4 Antibody (CL1115) [NBP2-52958]

Immunohistochemistry-Paraffin: BRD4 Antibody (CL1115) [NBP2-52958] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: BRD4 Antibody (CL1115) [NBP2-52958]

Immunohistochemistry-Paraffin: BRD4 Antibody (CL1115) [NBP2-52958]

Immunohistochemistry-Paraffin: BRD4 Antibody (CL1115) [NBP2-52958] - Staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.

Applications for BRD4 Antibody (CL1115) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BRD4

BRD4 is encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq]

Long Name

Bromodomain Containing 4

Alternate Names

HUNK1, HUNKI, MCAP

Gene Symbol

BRD4

Additional BRD4 Products

Product Documents for BRD4 Antibody (CL1115) - BSA Free

Certificate of Analysis

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Product Specific Notices for BRD4 Antibody (CL1115) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

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Protocols

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FAQs

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