Brg1 Antibody (BLR106H)

Novus Biologicals | Catalog # NBP3-14696

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR106H
View all Brg1 Primary Antibodies »

Product Specifications

Immunogen

between 75 and 125

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for Brg1 Antibody (BLR106H)

Western Blot: Brg1 Antibody (BLR106H) [NBP3-14696]

Western Blot: Brg1 Antibody (BLR106H) [NBP3-14696]

Western Blot: Brg1 Antibody (BLR106H) [NBP3-14696] - Whole cell lysate (50 ug) from Jurkat, K-562, HEK293T, MCF-7, HeLa, LNCaP, U2OS, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRG1recombinant monoclonal antibody [BLR106H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit antiCOPB2 antibody.
Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (BLR106H) [NBP3-14696]

Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (BLR106H) [NBP3-14696]

Immunocytochemistry/Immunofluorescence: Brg1 Antibody (BLR106H) [NBP3-14696] - Rabbit anti-BRG1 recombinant monoclonal antibody [BLR106H]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry-Paraffin: Brg1 Antibody (BLR106H) [NBP3-14696]

Immunohistochemistry-Paraffin: Brg1 Antibody (BLR106H) [NBP3-14696]

Immunohistochemistry-Paraffin: Brg1 Antibody (BLR106H) [NBP3-14696] - Antibody: Rabbit anti-BRG1 recombinant monoclonal antibody [BLR106H] Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.
Western Blot: Brg1 Antibody (BLR106H) [NBP3-14696]

Western Blot: Brg1 Antibody (BLR106H) [NBP3-14696]

Western Blot: Brg1 Antibody (BLR106H) [NBP3-14696] - Whole cell lysate (50 ug) from mIMCD-3 and A20 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRG1 recombinant monoclonal antibody [BLR106H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit antiCOPB2 antibody.
Immunoprecipitation: Brg1 Antibody (BLR106H) [NBP3-14696]

Immunoprecipitation: Brg1 Antibody (BLR106H) [NBP3-14696]

Immunoprecipitation: Brg1 Antibody [NBP3-14696] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-BRG1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. BRG1 was alsoimmunoprecipitated by rabbit anti-BRG1 antibody. For blotting immunoprecipitated BRG1, was used at 1:1000. Chemiluminescence withan exposure time of 3 seconds.

Applications for Brg1 Antibody (BLR106H)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

20 ul/1mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: Brg1

BRG1 is encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.

Long Name

BRM/SWI2-related gene 1

Alternate Names

SMARCA4, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2

Gene Symbol

SMARCA4

Additional Brg1 Products

Product Documents for Brg1 Antibody (BLR106H)

Certificate of Analysis

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Product Specific Notices for Brg1 Antibody (BLR106H)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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