Brg1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-22234

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all Brg1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 50 and 100 of human BRM/SWI2-related Gene 1 using the numbering given in entry NP_003063.2 (GeneID 6597).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Brg1 Antibody - BSA Free

Western Blot: Brg1 Antibody [NBP2-22234]

Western Blot: Brg1 Antibody [NBP2-22234]

Western Blot: Brg1 Antibody [NBP2-22234] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry: Brg1 Antibody [NBP2-22234]

Immunohistochemistry: Brg1 Antibody [NBP2-22234]

Immunohistochemistry: Brg1 Antibody [NBP2-22234] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- BRG1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry: Brg1 Antibody [NBP2-22234]

Immunohistochemistry: Brg1 Antibody [NBP2-22234]

Immunohistochemistry: Brg1 Antibody [NBP2-22234] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- BRG1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: Brg1 Antibody [NBP2-22234]

Immunoprecipitation: Brg1 Antibody [NBP2-22234]

Immunoprecipitation: Brg1 Antibody [NBP2-22234] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22234 used for IP at 6 ug/mg lysate. BRG1 was also immunoprecipitated by rabbit anti-BRG1 antibody NB100-2594. For blotting immunoprecipitated BRG1, NBP2-22234 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Brg1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:1000 - 1:5000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Brg1

BRG1 is encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.

Long Name

BRM/SWI2-related gene 1

Alternate Names

SMARCA4, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2

Gene Symbol

SMARCA4

UniProt

NP_003063.2 (Human)

Additional Brg1 Products

Product Documents for Brg1 Antibody - BSA Free

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Product Specific Notices for Brg1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Brg1 Antibody - BSA Free

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Protocols

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