Brg1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92957

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Brg1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 30-130 of human Brg1 (NP_003063.2). PSPGPSPGSAHSMMGPSPGPPSAGHPIPTQGPGGYPQDNMHQMHKPMESMHEKGMSDDPRYNQMKGMGMRSGGHAGMGPPPSPMDQHSQGYPSPLGGSEHA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Brg1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Brg1 Antibody - BSA Free [NBP2-92957]

Immunocytochemistry/ Immunofluorescence: Brg1 Antibody - BSA Free [NBP2-92957]

Immunocytochemistry/Immunofluorescence: Brg1 Antibody [NBP2-92957] - Analysis of HeLa cells using Brg1.
Knockout Validated: Brg1 Antibody - BSA Free [NBP2-92957]

Western Blot: Brg1 Antibody - BSA Free [NBP2-92957]

Western Blot: Brg1 Antibody [NBP2-92957] - Analysis of extracts from normal (control) and SMARCA4 knockout (KO) 293T cells, using Brg1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:Basic ECL Kit
Brg1 Antibody - BSA Free

Western Blot: Brg1 Antibody - BSA Free [NBP2-92957] -

Western Blot: Brg1 Antibody - BSA Free [NBP2-92957] - Western blot analysis of lysates from Hep G2 cells using [KO Validated] Brg1 Rabbit pAb at 1:1000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Applications for Brg1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20 - 1:50

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Brg1

BRG1 is encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.

Long Name

BRM/SWI2-related gene 1

Alternate Names

SMARCA4, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2

Gene Symbol

SMARCA4

Additional Brg1 Products

Product Documents for Brg1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Brg1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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