Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free

Novus Biologicals | Catalog # NB120-19347

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine

Cited:

Human, Mouse, Rat, Porcine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 5C10, ADD

Format

BSA Free
View all Calcium-sensing R/CaSR Primary Antibodies »

Product Specifications

Immunogen

Partial synthetic peptide corresponding to residues 214 - 235 of human calcium sensing receptor R / CaSR [UniProt P41180]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 21969374). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Porcine reactivity reported in scientific literature (PMID: 27836810).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free

Western Blot: Calcium-sensing R/CaSR Antibody (5C10, ADD)BSA Free [NB120-19347]

Western Blot: Calcium-sensing R/CaSR Antibody (5C10, ADD)BSA Free [NB120-19347]

Western Blot: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Total protein from human Hek293 and rat Pancreas was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CaSR in block buffer and detected with an anti-mouse HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.
Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free [NB120-19347]

Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free [NB120-19347]

Immunocytochemistry/Immunofluorescence: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Analysis of primary astrocytes using Calcium-sensing R/CaSR antibody. Primary astrocytes co-stained with cilia (Arl13b, red, top left), CASR (green, bottom left), DAPI (blue, top right) and merged (bottom right). Image from verified customer review.
Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free [NB120-19347]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free [NB120-19347]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Normal biopsies of deparaffinized Human kidney tissue.
Western Blot: Calcium-sensing R/CaSR Antibody (5C10, ADD)BSA Free [NB120-19347]

Western Blot: Calcium-sensing R/CaSR Antibody (5C10, ADD)BSA Free [NB120-19347]

Western Blot: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Analysis of calcium sensing receptor in HEK7-2 cell extract.
Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free [NB120-19347]

Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free [NB120-19347]

Immunocytochemistry/Immunofluorescence: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Analysis of bovine corneal epithelium (CE) limbus tissue.
Immunohistochemistry: Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free [NB120-19347]

Immunohistochemistry: Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free [NB120-19347]

Immunohistochemistry: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Fresh stomach tissue was fixed in 4% formalin for 1 hr & then incubated overnight at 4C in 25% sucrose before embedding in tissue freezing medium. Antigen retrieval was carried out on 8 um cryo-sections by incubating in sodium citrate buffer for 45 mins at 4C, immersing in sodium citrate buffer for 10 mins at 100C before washing 3 times for 5 mins each in 1X PBS. Blocked in blocking buffer (0.3% Triton X-100 in 1X PBS, 10% normal goat serum) for 30 mins at RT before staining with NB120-19347 (diluted 1:100 in blocking buffer) overnight at 4C followed by a fluorophore-conjugated goat anti-mouse IgG secondary antibody for 2 hrs at RT. Sections were also stained with DAPI nuclear stain (blue). NB120-19347 positive cells (green) were found at the base of the antral gl&s in the mouse stomach. Data courtesy of the Innovators Program
Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free [NB120-19347]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free [NB120-19347]

Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (5C10, ADD) [NB120-19347] - Normal biopsies of deparaffinized Human brain tissue.

Applications for Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500. Use reported in scientific literature

Western Blot

2 ug/ml
Application Notes
In Western blot this antibody detects bands at approximately 130 and 150 kDa. We have conflicting data on the use of this antibody in Flow.

Reviewed Applications

Read 1 review rated 5 using NB120-19347 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calcium-sensing R/CaSR

The calcium sensing receptor (CaSR) has been shown to play a major role in regulating parathyroid hormone secretion and subsequently influencing the calcium concentration of extracellular fluids. Based on sequence analysis of cDNA clones it appears that the CaSR is similar to the 7-transmembrane-domain G-protein-coupled receptor superfamily. Changes in extracellular calcium are thought to modulate a balance between proliferation and differentiation in a variety of cell types. In normal primary keratinocytes and breast epithelial cells, proliferation is inhibited and elevated extracellular calcium levels trigger differentiation. Malignant transformations of these cell types are accompanied by a loss of responsiveness to the anti-proliferative effects of elevated extracellular calcium. Several disorders of calcium homeostasis have been linked to mutations in the CaSR. These include familial hypocalciuric hypercalcemia (FHH), neonatal severe hyperparathyroidism (NSHPT), and autosomal dominant hypocalemia (ADHypo).

Long Name

Calcium-sensing Receptor

Alternate Names

CAR, CaSR, EIG8, FHH, FIH, GPRC2A, HHC1, NSHPT, PCaR1

Entrez Gene IDs

846 (Human); 24247 (Rat)

Gene Symbol

CASR

UniProt

P41180 (Rat)

Additional Calcium-sensing R/CaSR Products

Product Documents for Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free

Customer Reviews for Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Calcium-sensing R/CaSR Antibody (5C10, ADD)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: primary astrocytes
    Species: Human
    Verified Customer | Posted 02/25/2022
    Primary astrocytes co-stained with cilia (Arl13b, red, top left), CASR (green, bottom left), DAPI (blue, top right) and merged (bottom right).
    Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free NB120-19347

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

View specific protocols for Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free (NB120-19347):

Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and wash the cells briefly in PBS. Add 10% formalin to the dish and fix at room temperature for 10 minutes.
2. Remove the formalin and wash the cells in PBS.
3. Permeablize the cells with 0.1% Triton X100 or other suitable detergent for 10 min.
4. Remove the permeablization buffer and wash three times for 10 minutes each in PBS. Be sure to not let the specimen dry out.
5. To block nonspecific antibody binding, incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
6. Add primary antibody at appropriate dilution and incubate overnight at 4C.
7. Remove primary antibody and replace with PBS. Wash three times for 10 minutes each.
8. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
9. Remove secondary antibody and replace with PBS. Wash three times for 10 minutes each.
10. Counter stain DNA with DAPi if required.

Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes (keep slides in the sodium citrate buffer all the time).

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in PBS for 5 minutes.
3. Block each section with 100-400 ul blocking solution (1% BSA in PBS) for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul HRP polymer conjugated secondary antibody. Incubate 30 minutes at room temperature.
7. Wash sections three times in wash buffer for 5 minutes each.
8. Add 100-400 ul DAB substrate to each section and monitor staining closely.
9. As soon as the sections develop, immerse slides in deionized water.
10. Counterstain sections in hematoxylin.
11. Wash sections in deionized water two times for 5 minutes each.
12. Dehydrate sections.
13. Mount coverslips.


Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10-25 ug of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: Do you have a blocking peptide for NB120-19347?

    A: Unfortunately, no, the blocking peptide for the this antibody is not currently available.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies