|Detection of CD25/IL-2 R alpha in Lymphocytes in Canine PBMCs by Flow Cytometry. Lymphocytes in canine peripheral blood mononuclear cells were stained with Mouse Anti-Canine CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB2410A) and either (A) Sheep Anti-Canine CD25/IL-2 R alpha PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6227P) or (B) Normal Sheep IgG Phycoerythrin Control (Catalog # IC016P). View our protocol for Staining Membrane-associated Proteins.|
IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55-57 kDa type I transmembrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma c). IL‑2 R alpha is primarily expressed on activated T cells, mast cells and on regulatory T cells plus regulatory B cells (1-3). The canine IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 21 aa signal peptide, a 216 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and a 10 aa cytoplasmic domain (4). Within the ECD, canine IL-2 R alpha shares 49%-60% aa sequence identity with human, mouse, and rat IL-2 R alpha. IL-2 R beta (CD122) and gamma c (IL‑2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (1, 5, 6). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma c to generate a ternary high affinity IL-2 receptor complex (7). A soluble form of IL‑2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (8). In human, increased serum levels of soluble IL‑2 R alpha are found in some cancers and autoimmune disorders (9, 10). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (11, 12). IL-2 R alpha is also required for the development of CD4+CD25+ Treg which suppress autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (11-13).
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided