Canine CD25/IL-2 R alpha PE-conjugated Antibody

FAB6227P has been discontinued. View all CD25/IL-2 R alpha products.
  
  • Species Reactivity
    Canine
  • Specificity
    Detects canine IL-2 R alpha in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human IL-2 R alpha, recombinant mouse IL-2 R alpha, and recombinant rat IL-2 R alpha is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant canine IL-2 R alpha
    Tyr19-Ile238
    Accession # O62802
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD25/IL-2 R alpha in Lymphocytes in Canine PBMCs by Flow Cytometry. Lymphocytes in canine peripheral blood mononuclear cells were stained with Mouse Anti-Canine CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB2410A) and either (A) Sheep Anti-Canine CD25/IL-2 R alpha PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6227P) or (B) Normal Sheep IgG Phycoerythrin Control (Catalog # IC016P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD25/IL-2 R alpha

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55-57 kDa type I transmembrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma c). IL‑2 R alpha is primarily expressed on activated T cells, mast cells and on regulatory T cells plus regulatory B cells (1-3). The canine IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 21 aa signal peptide, a 216 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and a 10 aa cytoplasmic domain (4). Within the ECD, canine IL-2 R alpha shares 49%-60% aa sequence identity with human, mouse, and rat IL-2 R alpha. IL-2 R beta (CD122) and gamma c (IL‑2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (1, 5, 6). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma c to generate a ternary high affinity IL-2 receptor complex (7). A soluble form of IL‑2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (8). In human, increased serum levels of soluble IL‑2 R alpha are found in some cancers and autoimmune disorders (9, 10). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (11, 12). IL-2 R alpha is also required for the development of CD4+CD25+ Treg which suppress autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (11-13).

  • References:
    1. Bayer, A.L. et al. (2013) Immunol. Res. 57:197.
    2. Deho, L. et al. (2014) J. Immunol. 193:2196.
    3. Kessel, A. et al. (2012) Autoimmun. Rev. 11:670.
    4. Rissetto, K.C. et al. (2010) Vet. Immunol. Immunopathol. 135:137.
    5. Hatakeyama, M. et al. (1989) Science 244:551.
    6. Takeshita, T. et al. (1992) Science 257:379.
    7. Wang, X. et al. (2005) Science 310:1159.
    8. Schulz, O. et al. (1998) J. Exp. Med. 187:271.
    9. Russell, S.E. et al. (2012) PLoS ONE 7:e47748.
    10. Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121.
    11. Willerford, D.M. et al. (1995) Immunity 3:521.
    12. Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
    13. Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.
  • Long Name:
    Interleukin 2 Receptor alpha
  • Entrez Gene IDs:
    3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine)
  • Alternate Names:
    CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR
Related Research Areas

Have you used Canine CD25/IL-2 R alpha PE-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information