Canine CD25/IL-2 R alpha PE-conjugated Antibody
Canine CD25/IL-2 R alpha PE-conjugated Antibody Summary
Tyr19-Ile238
Accession # O62802
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD25/IL-2 R alpha in Lymphocytes in Canine PBMCs by Flow Cytometry. Lymphocytes in canine peripheral blood mononuclear cells were stained with Mouse Anti-Canine CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB2410A) and either (A) Sheep Anti-Canine CD25/IL-2 R alpha PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6227P) or (B) Normal Sheep IgG Phycoerythrin Control (Catalog # IC016P). View our protocol for Staining Membrane-associated Proteins.
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Background: CD25/IL-2R alpha
IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55-57 kDa type I transmembrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma c). IL‑2 R alpha is primarily expressed on activated T cells, mast cells and on regulatory T cells plus regulatory B cells (1-3). The canine IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 21 aa signal peptide, a 216 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and a 10 aa cytoplasmic domain (4). Within the ECD, canine IL-2 R alpha shares 49%-60% aa sequence identity with human, mouse, and rat IL-2 R alpha. IL-2 R beta (CD122) and gamma c (IL‑2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (1, 5, 6). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma c to generate a ternary high affinity IL-2 receptor complex (7). A soluble form of IL‑2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (8). In human, increased serum levels of soluble IL‑2 R alpha are found in some cancers and autoimmune disorders (9, 10). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (11, 12). IL-2 R alpha is also required for the development of CD4+CD25+ Treg which suppress autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (11-13).
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- Deho, L. et al. (2014) J. Immunol. 193:2196.
- Kessel, A. et al. (2012) Autoimmun. Rev. 11:670.
- Rissetto, K.C. et al. (2010) Vet. Immunol. Immunopathol. 135:137.
- Hatakeyama, M. et al. (1989) Science 244:551.
- Takeshita, T. et al. (1992) Science 257:379.
- Wang, X. et al. (2005) Science 310:1159.
- Schulz, O. et al. (1998) J. Exp. Med. 187:271.
- Russell, S.E. et al. (2012) PLoS ONE 7:e47748.
- Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121.
- Willerford, D.M. et al. (1995) Immunity 3:521.
- Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
- Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.
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