Canine CD25/IL-2 R alpha PE-conjugated Antibody

FAB6227P has been discontinued. View all CD25/IL-2 R alpha products.
Datasheet / CoA / SDS
Product Details
  • Species Reactivity
  • Specificity
    Detects canine IL-2 R alpha in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human IL-2 R alpha, recombinant mouse IL-2 R alpha, and recombinant rat IL-2 R alpha is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant canine IL-2 R alpha
    Accession # O62802
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
Product Datasheets

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  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CD25/IL-2 R alpha in Lymphocytes in Canine PBMCs by Flow Cytometry. Lymphocytes in canine peripheral blood mononuclear cells were stained with Mouse Anti-Canine CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB2410A) and either (A) Sheep Anti-Canine CD25/IL-2 R alpha PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6227P) or (B) Normal Sheep IgG Phycoerythrin Control (Catalog # IC016P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD25/IL-2 R alpha

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55-57 kDa type I transmembrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma c). IL‑2 R alpha is primarily expressed on activated T cells, mast cells and on regulatory T cells plus regulatory B cells (1-3). The canine IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 21 aa signal peptide, a 216 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and a 10 aa cytoplasmic domain (4). Within the ECD, canine IL-2 R alpha shares 49%-60% aa sequence identity with human, mouse, and rat IL-2 R alpha. IL-2 R beta (CD122) and gamma c (IL‑2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor (1, 5, 6). By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma c to generate a ternary high affinity IL-2 receptor complex (7). A soluble form of IL‑2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (8). In human, increased serum levels of soluble IL‑2 R alpha are found in some cancers and autoimmune disorders (9, 10). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (11, 12). IL-2 R alpha is also required for the development of CD4+CD25+ Treg which suppress autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (11-13).

  • References:
    1. Bayer, A.L. et al. (2013) Immunol. Res. 57:197.
    2. Deho, L. et al. (2014) J. Immunol. 193:2196.
    3. Kessel, A. et al. (2012) Autoimmun. Rev. 11:670.
    4. Rissetto, K.C. et al. (2010) Vet. Immunol. Immunopathol. 135:137.
    5. Hatakeyama, M. et al. (1989) Science 244:551.
    6. Takeshita, T. et al. (1992) Science 257:379.
    7. Wang, X. et al. (2005) Science 310:1159.
    8. Schulz, O. et al. (1998) J. Exp. Med. 187:271.
    9. Russell, S.E. et al. (2012) PLoS ONE 7:e47748.
    10. Witkowska, A.M. (2005) Mediat. Inflamm. 2005:121.
    11. Willerford, D.M. et al. (1995) Immunity 3:521.
    12. Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
    13. Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.
  • Long Name:
    Interleukin 2 Receptor alpha
  • Entrez Gene IDs:
    3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine)
  • Alternate Names:
    CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR
Related Research Areas


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