Canine IL-17 Antibody

Catalog # Availability Size / Price Qty
MAB5848
MAB5848-SP
IL-6 Secretion Induced by IL‑17 and Neutralization by Canine IL‑17 Antibody.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Canine IL-17 Antibody Summary

Species Reactivity
Canine
Specificity
Detects canine IL-17 in direct ELISAs. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) IL-17A is observed and no cross-reactivity with rhIL-17F or recombinant mouse IL-17A is observed.
Source
Monoclonal Mouse IgG2A Clone # 665909
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant canine IL-17
Gly26-Ala155
Accession # NP_001159350
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize IL‑17-induced IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811.The Neutralization Dose (ND50) is typically 5-30 ng/mL in the presence of 5 ng/mL Recombinant Canine IL‑17.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization IL-6 Secretion Induced by IL‑17 and Neutralization by Canine IL‑17 Antibody. View Larger

IL-6 Secretion Induced by IL‑17 and Neutralization by Canine IL‑17 Antibody. Recombinant Canine IL-17 (Catalog # 5848-CL) induces IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). IL-6 Secretion elicited by Recombinant Canine IL-17 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL-17 Monoclonal Antibody (Catalog # MAB5848). The ND50 is typically 5-30 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17/IL-17A

Interleukin 17 (IL-17; also IL-17A and CTLA-8) is a 17 kDa member of the IL-17 family of cytokines (1). Members of this family demonstrate a structural motif termed a cysteine knot which characterize a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (2-4). Based on the amino acid (aa) sequence alignment with human IL-17, canine IL-17 is 130 aa in length. It is secreted as a 35 kDa disulfide-linked homodimer and as a 40 kDa disulfide-linked heterodimer with IL-17F (5). Canine IL‑17 is 81% identical on the aa level to human IL-17. IL-23 drives Th17 lymphocytes to produce IL-17 (6-8). IL-17’s production has also been demonstrated in gamma δ T cells (9), CD8+ memory T cells (10-11), eosinophils (12), neutrophils (10), and monocytes (13). Studies have identified that the widely expressed receptors IL‑17RA and IL-17RC form a heterodimer for the binding of IL-17 (6, 14-15).  The predominant function of IL-17 is thought to be as a proinflammatory mediator through a variety of mechanisms (16). Locally, IL‑17 stimulates production of IL-6, prostaglandin E and nitric oxide (16-19), and synergy with other inflammatory cytokines such as TNF-alpha, IL‑1 beta and IFN -gamma leads to up-regulation of gene expression and progression and amplification of local inflammation (16, 20-22). IL‑17 also mediates chemotaxis of neutrophils and monocytes to sites of inflammation through the chemoattractant mediators IL-8, GRO‑ alpha, and MCP-1 (16, 22-25) while augmenting production of hematopoietic growth factors, such as G-CSF and GM-CSF (16, 26, 27), which promote the growth and maturation of the recruited myeloid cells. In addition, IL-17 serves as a bridge between innate and adaptive immune responses by enhancing the induction of co-stimulatory molecules such as ICAM-1 and other cytokines (16, 22, 28), thereby supporting T cell activation. IL-17 expression has been associated with many inflammatory diseases, such as rheumatoid arthritis, multiple sclerosis, asthma, systemic lupus erythematosus and allograft rejection (15).

References
  1. Gaffen, S.L. et al. (2006) Vitam. Horm. 74:255.
  2. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
  3. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
  4. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
  5. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
  6. Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
  7. Steinman, L. (2007) Nat. Med. 13:139.
  8. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
  9. Lockhart, E. et al. (2006) J. Immunol. 177:4662.
  10. Ferretti, S. et al. (2003) J. Immunol. 170:2106.
  11. Shin, H.C. et al. (1999) Cytokine 11:257.
  12. Molet, S. et al. (2001) J. Allergy Clin. Immunol. 108:430.
  13. Zhou, Q. et al. (2005) Infect. Immun. 73:935.
  14. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
  15. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
  16. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  17. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
  18. Yao, Z. et al. (1995) Immunity 3:811.
  19. Attur, M.G. et al. (1997) Arthritis Rheum. 40:1050.
  20. Ruddy, M.J. et al. (2004) J. Biol. Chem. 279:2559.
  21. Albanesi, C. et al. (1999) J. Immunol. 162:494.
  22. Witowski, J. et al. (2000) J. Immunol. 165:5814.
  23. Miyamoto, M. et al. (2003) J. Immunol. 170:4665.
  24. Ye, P. et al. (2001) Am. J. Respir. Cell Mol. Biol. 25:335.
  25. Laan, M. et al. (2001) Br. J. Pharmacol. 133:200.
  26. Starnes, T. et al. (2002) J. Immunol. 169:642.
  27. Jones, C.E. et al. (2002) Am. J. Respir. Cell Mol. Biol. 26:748.
  28. Yao, Z. et al. (1995) J. Immunol. 155:5483.
Long Name
Interleukin 17
Entrez Gene IDs
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Canine IL-17 Antibody

There are currently no reviews for this product. Be the first to review Canine IL-17 Antibody and earn rewards!

Have you used Canine IL-17 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review