Canine IL-17 Antibody

    
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  • Species Reactivity
    Canine
  • Specificity
    Detects canine IL-17 in direct ELISAs. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) IL-17A is observed and no cross-reactivity with rhIL-17F or recombinant mouse IL-17A is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 665909
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant canine IL-17
    Gly26-Ala155
    Accession # NP_001159350
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize IL‑17-induced IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811.The Neutralization Dose (ND50) is typically 5-30 ng/mL in the presence of 5 ng/mL Recombinant Canine IL‑17.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
IL-6 Secretion Induced by IL‑17 and Neutralization by Canine IL‑17 Antibody.

Recombinant Canine IL‑17 (Catalog # 5848-CL) induces IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). IL-6 Secretion elicited by Recombinant Canine IL‑17 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL‑17 Monoclonal Antibody (Catalog # MAB5848). The ND50 is typically 5-30 ng/mL.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17/IL-17A

Interleukin 17 (IL-17; also IL-17A and CTLA-8) is a 17 kDa member of the IL-17 family of cytokines (1). Members of this family demonstrate a structural motif termed a cysteine knot which characterize a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (2-4). Based on the amino acid (aa) sequence alignment with human IL-17, canine IL-17 is 130 aa in length. It is secreted as a 35 kDa disulfide-linked homodimer and as a 40 kDa disulfide-linked heterodimer with IL-17F (5). Canine IL‑17 is 81% identical on the aa level to human IL-17. IL-23 drives Th17 lymphocytes to produce IL-17 (6-8). IL-17’s production has also been demonstrated in gamma δ T cells (9), CD8+ memory T cells (10-11), eosinophils (12), neutrophils (10), and monocytes (13). Studies have identified that the widely expressed receptors IL‑17RA and IL-17RC form a heterodimer for the binding of IL-17 (6, 14-15).  The predominant function of IL-17 is thought to be as a proinflammatory mediator through a variety of mechanisms (16). Locally, IL‑17 stimulates production of IL-6, prostaglandin E and nitric oxide (16-19), and synergy with other inflammatory cytokines such as TNF-alpha, IL‑1 beta and IFN -gamma leads to up-regulation of gene expression and progression and amplification of local inflammation (16, 20-22). IL‑17 also mediates chemotaxis of neutrophils and monocytes to sites of inflammation through the chemoattractant mediators IL-8, GRO‑ alpha, and MCP-1 (16, 22-25) while augmenting production of hematopoietic growth factors, such as G-CSF and GM-CSF (16, 26, 27), which promote the growth and maturation of the recruited myeloid cells. In addition, IL-17 serves as a bridge between innate and adaptive immune responses by enhancing the induction of co-stimulatory molecules such as ICAM-1 and other cytokines (16, 22, 28), thereby supporting T cell activation. IL-17 expression has been associated with many inflammatory diseases, such as rheumatoid arthritis, multiple sclerosis, asthma, systemic lupus erythematosus and allograft rejection (15).

  • References:
    1. Gaffen, S.L. et al. (2006) Vitam. Horm. 74:255.
    2. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
    3. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
    4. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
    5. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
    6. Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
    7. Steinman, L. (2007) Nat. Med. 13:139.
    8. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
    9. Lockhart, E. et al. (2006) J. Immunol. 177:4662.
    10. Ferretti, S. et al. (2003) J. Immunol. 170:2106.
    11. Shin, H.C. et al. (1999) Cytokine 11:257.
    12. Molet, S. et al. (2001) J. Allergy Clin. Immunol. 108:430.
    13. Zhou, Q. et al. (2005) Infect. Immun. 73:935.
    14. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
    15. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
    16. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
    17. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
    18. Yao, Z. et al. (1995) Immunity 3:811.
    19. Attur, M.G. et al. (1997) Arthritis Rheum. 40:1050.
    20. Ruddy, M.J. et al. (2004) J. Biol. Chem. 279:2559.
    21. Albanesi, C. et al. (1999) J. Immunol. 162:494.
    22. Witowski, J. et al. (2000) J. Immunol. 165:5814.
    23. Miyamoto, M. et al. (2003) J. Immunol. 170:4665.
    24. Ye, P. et al. (2001) Am. J. Respir. Cell Mol. Biol. 25:335.
    25. Laan, M. et al. (2001) Br. J. Pharmacol. 133:200.
    26. Starnes, T. et al. (2002) J. Immunol. 169:642.
    27. Jones, C.E. et al. (2002) Am. J. Respir. Cell Mol. Biol. 26:748.
    28. Yao, Z. et al. (1995) J. Immunol. 155:5483.
  • Long Name:
    Interleukin 17
  • Entrez Gene IDs:
    3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)
  • Alternate Names:
    CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A
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Isotype Controls
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Mouse IgG2A Isotype Control

Ctrl MAB003 35  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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WB BAF018 1
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Mouse IgG Antibody

WB AF007 4
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG2A Antibody

WB , Flow MAB0033  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Mouse IgG2A Antibody

Flow MAB0032  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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