Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Simple Western

Cited:

Immunohistochemistry-Frozen, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Carbonic Anhydrase II/CA2 Primary Antibodies »

Product Specifications

Immunogen

Carbonic Anhydrase II/CA2 Antibody was produced by repeated immunizations with human erythrocytes Carbonic Anhydrase II/CA2. (Uniprot: P00918)

Reactivity Notes

Cross reactivity against Carbonic Anhydrase II/CA2 from other tissues and species may occur but have not been specifically determined.

Localization

Cytoplasmic

Specificity

Cross reactivity against Carbonic Anhydrase II/CA2 from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Human Erythrocytes]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Carbonic Anhydrase II/CA2 Antibody - BSA Free

Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NB600-919-img0008.jpg
Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Staining in nucleus and cytoplasm of proximal and distal tubules, bowman's capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 ug/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3'3-diamino-benzidine substrate (DAB) or WARP RED.
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Detection of Carbonic Anhydrase II in mouse embryonic whole cell lysate. Image provided via product review by verified customer.
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - analysis of Carbonic Anhydrase II protein (loading amount: 50 ng) using rabbit anti-Carbonic Anhydrase II antibody (NB600-919) at a dilution of 1:1000, incubation - overnight at 4C.
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Analysis using the Biotin conjugate of NB600-919. Detection of Lane 1: Carbonic Anhydrase II. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carbonic Anhydrase II Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptav
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Lane 1: Carbonic Anhydrase II. Lane 2: None. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase II primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase II. Other band(s): None.
Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Localization of CAII in mouse peritoneum. Image from verified customer review.
Carbonic Anhydrase II/CA2 Antibody

Carbonic Anhydrase II/CA2 Antibody

Immunohistochemistry with anti-carbonic anhydrase II antibody showing carbonic anhydrase II staining in nucleus and cytoplasm of proximal and distal tubules, bowman's capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 ug/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3'3-diamino-benzidine substrate (DAB) or WARP RED.

Applications for Carbonic Anhydrase II/CA2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:165000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Simple Western

1:400

Western Blot

1:2000-1:10000
Application Notes
This product has been tested by ELISA, western blot, and immunohistochemistry. Expect ~29.2kDa. Researchers should determine optimal titers for applications that are not stated below.

Use in Immunohistochemistry-Frozen reported in scientific literature (PMID
See Simple Western Antibody Database for Simple Western validation: Tested in Blood, separated by Size, antibody dilution of 1:400

Reviewed Applications

Read 3 reviews rated 5 using NB600-919 in the following applications:

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carbonic Anhydrase II/CA2

Carbonic Anhydrase II Antibody detects Carbonic Anhydrase II. Carbonic Anhydrase II is essential for bone resorption and osteoclast differentiation by similarity. It can hydrate cyanamide to urea and is involved in the regulation of fluid secretion into the anterior chamber of the eye. Anti-Carbonic Anhydrase II Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Alternate Names

CA2

Gene Symbol

CA2

UniProt

P00918

Additional Carbonic Anhydrase II/CA2 Products

Product Documents for Carbonic Anhydrase II/CA2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Carbonic Anhydrase II/CA2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glial Lineage Markers

Citations for Carbonic Anhydrase II/CA2 Antibody - BSA Free

Customer Reviews for Carbonic Anhydrase II/CA2 Antibody - BSA Free (3)

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Name: Amadou Sow
    Application: Immunohistochemistry
    Sample Tested: Mouse peritoneum
    Species: Mouse
    Verified Customer | Posted 09/23/2014
    localization of CAII in mouse peritoneum
    Carbonic Anhydrase II/CA2 Antibody - BSA Free NB600-919
  • Name: Corey.
    Application: Western Blot
    Sample Tested: Embryonic gestational day 14.5 mouse whole embryo lysates
    Species: Mouse
    Verified Customer | Posted 06/24/2013
    Western blot analysis of mouse embryonic whole cell lysates
    Carbonic Anhydrase II/CA2 Antibody - BSA Free NB600-919
  • Name: Susan Frost
    Application: Western Blot
    Sample Tested: Detection of CA11 in human cancer cells
    Species: Human
    Verified Customer | Posted 10/06/2011

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Carbonic Anhydrase II/CA2 Antibody - BSA Free

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  • Q: I am going to perform CA2 / Carbonic Anhydrase II immunohistochemistry staining for bone samples. Is this antibody better for plastic embedded bone samples? Or does it only work on paraffin embedded samples or soft tissue?

    A: NB600-919 has only been tested on paraffin-embedded tissues. It will most likely work on your samples, but it has never been tested in this application specifically. From our catalog, this antibody is the best choice for your experiments.

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