Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free

Novus Biologicals | Catalog # NBP1-51691

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2D3

Format

BSA Free
View all Carbonic Anhydrase IX/CA9 Primary Antibodies »

Product Specifications

Immunogen

This Carbonic Anhydrase IX/CA9 Antibody (2D3) was made to a purified recombinant fragment of human Carbonic Anhydrase IX expressed in E. coli [UniProt# Q16790].

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 33153038).

Marker

Hypoxia Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free

Western Blot: Carbonic Anhydrase IX/CA9 Antibody (2D3)BSA Free [NBP1-51691]

Western Blot: Carbonic Anhydrase IX/CA9 Antibody (2D3)BSA Free [NBP1-51691]

Western Blot: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - Carbonic Anhydrase IX mouse antibody against Hela (1) and A549 (2) cell lysates. Bands were detected at a molecular weight of approximately 50 kDa in both cell lines.
Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]

Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]

Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - Paraffin-embedded lung tissues (left) and colonic tissues (right) using Carbonic Anhydrase IX mouse antibody with DAB staining.
Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]

Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]

Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Carbonic Anhydrase IX/CA9 Antibody [2D3] (NBP1-51691) at 2ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]

Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]

Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - A surface stain was performed on A431 cells with Carbonic Anhydrase IX/CA9 NBP1-51691 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by DyLight488-conjugated anti-mouse secondary antibody.
Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]

Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]

Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - Mouse choroid stained with anti-Carbonic Anhydrase IX antibody. ICC/IF image submitted by a verified customer review.
Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]

Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]

Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - Analysis of NTERA-2 cells using Carbonic Anhydrase IX mouse mAb (green) and negative control (purple).

Applications for Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow (Intracellular)

1 ug/mL

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:2000

Reviewed Applications

Read 2 reviews rated 5 using NBP1-51691 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase IX/CA9

Carbonic anhydrase alpha, isozyme IX, belongs to a family of zinc-containing metalloproteins which hydrate carbon dioxide to generate bicarbonate ions and protons (1). This main catalytic function allows carbonic anhydrase IX to participate in cellular pH regulation. The large family of carbonic anhydrase metalloproteins includes three major classes which have been identified based on sequence and structure analysis. The alpha class is a monomer found in mammals. The beta class may occur as a dimer, tetramer, hexamer or octamer and is found in plants, algae, and bacteria. Lastly, the gamma class is a trimer found in bacteria and represents the most ancient carbonic anhydrase. These three classes of carbonic anhydrase enzymes lack sequence or structural similarities, but all share a conserved active site zinc atom (1).

Carbonic anhydrase IX (theoretical molecular weight 50kDa) belongs to the monomeric alpha class and is a single pass-transmembrane protein with two extracellular domains which serve catalytic and cell adhesion functions (2, 3). By cooperating with sodium bicarbonate cotransporters (NBC), lactate and proton exporting monocarboxylic acid transporters (MCT), and a sodium/hydrogen exchanger (NHE), carbonic anhydrase IX is involved in pH regulation across the cell membrane. This functional property protects cancer cells from intracellular acidification and partly explains the role of carbonic anhydrase IX in cancer cell survival and proliferation. In contrast, the pH regulating activity of carbonic anhydrase IX induces extracellular acidification, which has been implicated in epithelial to mesenchymal transition (EMT) and promoting cancer invasion. Carbonic anhydrase IX is frequently overexpressed in cancer cells (e.g., colorectal-, breast-, lung-carcinoma and brain tumors), an effect promoted by hypoxia within the tumor microenvironment (4). An exception are tumors carrying pVHL inactivating mutations, such as clear cell renal cell carcinoma (ccRCC), where HIF-alpha is stabilized due to dysfunctional proteasomal targeting and can induce HRE (Hypoxia Response Element) containing genes even under physiological normoxia (5). Carbonic anhydrase IX may be detected by immunostaining in tumors, which is found in association with necrotic tissue and metastatic cells. Because the expression of carbonic anhydrase IX correlates with both tumor grade and stage, analysis of its expression in tumors serves as a prognostic factor (4, 6).

References

1. Tripp, B. C., Smith, K., & Ferry, J. G. (2001). Carbonic Anhydrase: New Insights for an Ancient Enzyme. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.R100045200

2. Nishimori, I., & Onishi, S. (2001). Carbonic anhydrase isozymes in the human pancreas. Digestive and Liver Disease. https://doi.org/10.1016/s1590-8658(01)80138-9

3. Zavadova, Z., & Zavada, J. (2005). Carbonic anhydrase IX (CA IX) mediates tumor cell interactions with microenvironment. Oncology Reports. https://doi.org/10.3892/or.13.5.977

4. Pastorekova, S., & Gillies, R. J. (2019). The role of carbonic anhydrase IX in cancer development: links to hypoxia, acidosis, and beyond. Cancer and Metastasis Reviews. https://doi.org/10.1007/s10555-019-09799-0

5. Haase, V. (2009). The VHL Tumor Suppressor: Master Regulator of HIF. Current Pharmaceutical Design. https://doi.org/10.2174/138161209789649394

6. Young, J. R., Coy, H., Kim, H. J., Douek, M., Sisk, A., Pantuck, A. J., & Raman, S. S. (2018). Association of the gross appearance of intratumoral vascularity at MDCT with the carbonic anhydrase IX score in clear cell renal cell carcinoma. American Journal of Roentgenology. https://doi.org/10.2214/AJR.18.19725

Alternate Names

CA9, G250, MN, RCC

Entrez Gene IDs

768 (Human)

Gene Symbol

CA9

UniProt

Q16790

Additional Carbonic Anhydrase IX/CA9 Products

Product Documents for Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Citations for Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free

Customer Reviews for Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Carbonic Anhydrase IX/CA9 Antibody (2D3)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293
    Species: Human
    Verified Customer | Posted 07/05/2021
    Carbonic Anhydrase IX/CA9 Antibody #1: HEK293 #2: HEK293
    Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free NBP1-51691
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: MCF-7 whole cell lysate
    Species: Human
    Verified Customer | Posted 11/20/2015
    Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free NBP1-51691

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free

Showing  1 - 3 of 3 FAQs Showing All

  • Q: I am looking for a primary antibody to Carbonic Anhydrase 9 and I want an antibody that recognises an extracellular epitope so that I can use it for live cell flow cytometry. Would #NBP1-51691 be a suitable candidate?

    A: The immunogen of our Carbonic Anhydrase IX antibody (clone 2D3) # NBP1-51691 corresponds to peptide from amino acids region between 40-290 of human CA9, which appears to be in the extracellular domain of this protein (based on UniProt: Q16790). In our QC lab, this antibody has been validated for FLOW application, so that you may use this antibody for your current experiments.

  • Q: We are looking for the antibody available for cell sorting by CA9. The sorted cells must be cultured for the expansion after the sorting for the following experiments. Do you have the antibodies useful for this?

    A: We sell two carbonic anhydrase IX antibodies that have been validated for Flow Cytometry detection of the human protein. These have catalogue numbers NBP1-51691 and NBP1-47688 and can be seen at the following link: carbonic anhydrase IX antibodies. Since both of these products contain sodium azide as a preservative, you are unlikely to be able to culture the cells on following sorting unless you performed a buffer exchange prior to using the antibody. Unfortunately we have no direct experience with either of these products of staining and subsequently expanding cell populations.

  • Q: What is the predicted theoretical molecular weight of Carbonic Anhydrase IX/CA9 antibodies?

    A: The TMW for Carbonic Anhydrase IX/CA9 antibodies is 50 kDa. Please note the observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

  • Q: I am looking for a primary antibody to Carbonic Anhydrase 9 and I want an antibody that recognises an extracellular epitope so that I can use it for live cell flow cytometry. Would #NBP1-51691 be a suitable candidate?

    A: The immunogen of our Carbonic Anhydrase IX antibody (clone 2D3) # NBP1-51691 corresponds to peptide from amino acids region between 40-290 of human CA9, which appears to be in the extracellular domain of this protein (based on UniProt: Q16790). In our QC lab, this antibody has been validated for FLOW application, so that you may use this antibody for your current experiments.

  • Q: We are looking for the antibody available for cell sorting by CA9. The sorted cells must be cultured for the expansion after the sorting for the following experiments. Do you have the antibodies useful for this?

    A: We sell two carbonic anhydrase IX antibodies that have been validated for Flow Cytometry detection of the human protein. These have catalogue numbers NBP1-51691 and NBP1-47688 and can be seen at the following link: carbonic anhydrase IX antibodies. Since both of these products contain sodium azide as a preservative, you are unlikely to be able to culture the cells on following sorting unless you performed a buffer exchange prior to using the antibody. Unfortunately we have no direct experience with either of these products of staining and subsequently expanding cell populations.

  • Q: What is the predicted theoretical molecular weight of Carbonic Anhydrase IX/CA9 antibodies?

    A: The TMW for Carbonic Anhydrase IX/CA9 antibodies is 50 kDa. Please note the observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

  • Q: I am looking for a primary antibody to Carbonic Anhydrase 9 and I want an antibody that recognises an extracellular epitope so that I can use it for live cell flow cytometry. Would #NBP1-51691 be a suitable candidate?

    A: The immunogen of our Carbonic Anhydrase IX antibody (clone 2D3) # NBP1-51691 corresponds to peptide from amino acids region between 40-290 of human CA9, which appears to be in the extracellular domain of this protein (based on UniProt: Q16790). In our QC lab, this antibody has been validated for FLOW application, so that you may use this antibody for your current experiments.

  • Q: We are looking for the antibody available for cell sorting by CA9. The sorted cells must be cultured for the expansion after the sorting for the following experiments. Do you have the antibodies useful for this?

    A: We sell two carbonic anhydrase IX antibodies that have been validated for Flow Cytometry detection of the human protein. These have catalogue numbers NBP1-51691 and NBP1-47688 and can be seen at the following link: carbonic anhydrase IX antibodies. Since both of these products contain sodium azide as a preservative, you are unlikely to be able to culture the cells on following sorting unless you performed a buffer exchange prior to using the antibody. Unfortunately we have no direct experience with either of these products of staining and subsequently expanding cell populations.

  • Q: What is the predicted theoretical molecular weight of Carbonic Anhydrase IX/CA9 antibodies?

    A: The TMW for Carbonic Anhydrase IX/CA9 antibodies is 50 kDa. Please note the observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Showing  1 - 3 of 3 FAQs Showing All
View all FAQs for Antibodies