Casein Kinase 1 epsilon Antibody

Novus Biologicals | Catalog # NB100-93388

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Casein Kinase 1 epsilon Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PASQTSVPFDHLGK corresponding to C-Terminus according to NP_689407.1.

Epitope

C-PASQTSVPFDHLGK

Specificity

Both reported variants represent identical protein (NP_001885.1 and NP_689407.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Casein Kinase 1 epsilon Antibody

Western Blot: Casein Kinase 1 epsilon Antibody [NB100-93388]

Western Blot: Casein Kinase 1 epsilon Antibody [NB100-93388]

Western Blot: Casein Kinase 1 epsilon Antibody [NB100-93388] - (1ug/ml) staining of A431(A), HeLa (B) and K562 (C) nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Casein Kinase 1 epsilon Antibody [NB100-93388]

Immunocytochemistry/ Immunofluorescence: Casein Kinase 1 epsilon Antibody [NB100-93388]

Immunocytochemistry/Immunofluorescence: Casein Kinase 1 epsilon Antibody [NB100-93388] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: Casein Kinase 1 epsilon Antibody [NB100-93388]

Immunohistochemistry-Paraffin: Casein Kinase 1 epsilon Antibody [NB100-93388]

Immunohistochemistry-Paraffin: Casein Kinase 1 epsilon Antibody [NB100-93388] - Staining of paraffin embedded human tonsil tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: Casein Kinase 1 epsilon Antibody [NB100-93388]

Flow Cytometry: Casein Kinase 1 epsilon Antibody [NB100-93388]

Flow Cytometry: Casein Kinase 1 epsilon Antibody [NB100-93388] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: Casein Kinase 1 epsilon Antibody [NB100-93388]

Immunocytochemistry/ Immunofluorescence: Casein Kinase 1 epsilon Antibody [NB100-93388]

Immunocytochemistry/Immunofluorescence: Casein Kinase 1 epsilon Antibody [NB100-93388] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for Casein Kinase 1 epsilon Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/mL

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Casein Kinase 1 epsilon

CKI epsilon is a member of the casein kinase 1 (CK1) serine/threonine kinase family of proteins. In mammals, there are seven distinct genes that represent the CK1 family: alpha, beta, gamma-1, gamma-2, gamma-3, delta, and epsilon. CKI epsilon phosphorylates a large number of proteins and is involved in pathways involving Wnt and AKT. CKI epsilon has been found to bind and phosphorylate the circadian protein, Period, and the Wnt signaling protein, Dsh. In the Wnt pathway, CKI epsilon activity is required for the full activation of Dsh and downstream signaling effects.

Alternate Names

CSNK1E

Entrez Gene IDs

1454 (Human); 27373 (Mouse); 58822 (Rat)

Gene Symbol

CSNK1E

UniProt

NP_001885.1 (Human)

Additional Casein Kinase 1 epsilon Products

Product Documents for Casein Kinase 1 epsilon Antibody

Certificate of Analysis

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Product Specific Notices for Casein Kinase 1 epsilon Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

Citations for Casein Kinase 1 epsilon Antibody

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Protocols

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