Caspase-11 Antibody (17D9) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80612
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Human, Mouse, Rat, Bovine
Applications
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoblotting, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray, Knockdown Validated, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 17D9
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
A recombinant p30 subunit of mouse Caspase 11. [UniProt# P70343]
Localization
Cytoplasmic (Endoplasmic reticulum membrane and Mitochondrion)
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Caspase-11 Antibody (17D9) - Azide and BSA Free
![Western Blot: Caspase-11 Antibody (17D9)Azide and BSA Free [NBP2-80612]](https://resources.rndsystems.com/images/products/Caspase-11-Antibody-17D9-Azide-and-BSA-Free-Western-Blot-NBP2-80612-img0003.jpg "Western Blot: Caspase-11 Antibody (17D9)Azide and BSA Free [NBP2-80612]")
Western Blot: Caspase-11 Antibody (17D9)Azide and BSA Free [NBP2-80612]
Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - Mouse liver extract was separated on a 4-15% gel and transfered to PVDF membrane. The membrane was probed with anti-caspase 11 antibody and detected with an anti-mouse HRP secondary antibody. Both procaspase 11 and fully cleaved caspase 11 are detected. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]](https://resources.rndsystems.com/images/products/Caspase-11-Antibody-17D9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80612-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]")
Immunocytochemistry/ Immunofluorescence: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]
Immunocytochemistry/Immunofluorescence: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - Caspase 11 antibody was tested in NIH-3T3 cells at a 1:100 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI. Image from the standard format of this antibody.![Immunohistochemistry: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]](https://resources.rndsystems.com/images/products/Caspase-11-Antibody-17D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80612-img0005.jpg "Immunohistochemistry: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]")
Immunohistochemistry: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]
Immunohistochemistry: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of mouse lung using Caspase 11 antibody (clone 17D9) at 1:100 diluton. The antibody generated cytoplasmic signal with more intense staining in the bronchiolar epithelium while the stainin![Western Blot: Caspase-11 Antibody (17D9)Azide and BSA Free [NBP2-80612]](https://resources.rndsystems.com/images/products/Caspase-11-Antibody-17D9-Azide-and-BSA-Free-Western-Blot-NBP2-80612-img0001.jpg "Western Blot: Caspase-11 Antibody (17D9)Azide and BSA Free [NBP2-80612]")
Western Blot: Caspase-11 Antibody (17D9)Azide and BSA Free [NBP2-80612]
Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - analysis of Caspase 11 in LPS stimulated bone marrow derived macrophages using anti-Caspase 11 antibody. Image from verified customer review. Image from the standard format of this antibody.![Western Blot: Caspase-11 Antibody (17D9)Azide and BSA Free [NBP2-80612]](https://resources.rndsystems.com/images/products/Caspase-11-Antibody-17D9-Azide-and-BSA-Free-Western-Blot-NBP2-80612-img0002.jpg "Western Blot: Caspase-11 Antibody (17D9)Azide and BSA Free [NBP2-80612]")
Western Blot: Caspase-11 Antibody (17D9)Azide and BSA Free [NBP2-80612]
Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - Analysis of Caspase-11 in THP-1 cell lysates that were untreated (left lane) or treated (right lane) with 100 ng/ml LPS. Image from verified customer review. Image from the standard format of this antibody.![Knockdown Validated: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]](https://resources.rndsystems.com/images/products/Caspase-11-Antibody-17D9-Azide-and-BSA-Free-Knockdown-Validated-NBP2-80612-img0006.jpg "Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]")
Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]
Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - Casp11fl/fl and Casp11EC-/- mice (n = 3) were challenged with LPS (40 mg/kg i.p.) for 6 hours. Endothelial Cells were isolated from lungs, and western blot analysis was performed for pro-IL-1Beta, mature IL-1Beta, and caspase-1 cleavage. This image is from PMID: 28990935. Image from the standard format of this antibody.![Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]](https://resources.rndsystems.com/images/products/Caspase-11-Antibody-17D9-Azide-and-BSA-Free-Knockout-Validated-NBP2-80612-img0007.jpg "Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]")
Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]
Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - Caspase-11 is expressed in Casp1Null bone marrow-derived macrophages. Immunoblot analysis of caspase-1, caspase-11 and GAPDH (loading control) in unprimed WT at various times (above lane) after stimulation with IFN-beta (250U/ml), IFN-gamma (100 ng/ml) or LPS (100 ng/ml). Data are representative of two independent experiments. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep45126), licensed under a CC-BY license. Image from the standard format of this antibody.![Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]](https://resources.rndsystems.com/images/products/Caspase-11-Antibody-17D9-Azide-and-BSA-Free-Knockout-Validated-NBP2-80612-img0009.jpg "Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]")
Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]
Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - CNF1-triggered immunity requires inflammatory caspases.(A and B) IL-1beta production and maturation/secretion after treatment with CNF1, LPS or CNF1 (1 ug/ml) + LPS (100 ng/ml) for 10 h. Actin and BSA were used as loading controls. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1004732), licensed under a CC-BY license. Image from the standard format of this antibody.![Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]](https://resources.rndsystems.com/images/products/Caspase-11-Antibody-17D9-Azide-and-BSA-Free-Knockout-Validated-NBP2-80612-img0008.jpg "Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]")
Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612]
Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - CNF1-triggered IL-1beta maturation requires activated Rac, ASC and caspase-1. Western blot analysis of the production and maturation/secretion of IL-1beta by primary macrophages following treatment with CNF1, LPS or CNF1+LPS for 10 h. Actin and BSA were used as loading controls. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1004732), licensed under a CC-BY license. Image from the standard format of this antibody.Applications for Caspase-11 Antibody (17D9) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
reported in scientific literature (PMID 27708283)
Immunoblotting
reported in scientific literature (PMID 28345580)
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:100
Immunoprecipitation
reported in scientific literature (PMID 10791975)
Western Blot
5 ug/ml
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Caspase-11
Alternate Names
CASP11, CASP-11, CASP4, CASP-4, caspase 4, apoptosis-related cysteine peptidase, Caspl, ich-3
Gene Symbol
Casp4
Additional Caspase-11 Products
Product Documents for Caspase-11 Antibody (17D9) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Caspase-11 Antibody (17D9) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for Caspase-11 Antibody (17D9) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review Caspase-11 Antibody (17D9) - Azide and BSA Free and earn rewards!
Have you used Caspase-11 Antibody (17D9) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...