Caspase-8 Antibody (90A992) [Biotin]

Novus Biologicals | Catalog # NB100-55786

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Primate

Applications

Western Blot, ELISA

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 90A992
View all Caspase-8 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product falls between amino acids 360-385 of the human Caspase 8 protein.

Reactivity Notes

Chimpanzee and Rhesus Monkey reactivity noted.

Specificity

This can be used for detection of the pro-form of Caspase-8.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Caspase-8 Antibody (90A992) [Biotin]

Western Blot: Caspase-8 Antibody (90A992) [Biotin] [NB100-55786] - Analysis for human Caspase-8 using Jurkat lysates with NB100-55786 at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2) dilution. NB100-55786 only detects 55 kDa Caspase-8 in Jurkat cells.

Applications for Caspase-8 Antibody (90A992) [Biotin]

Application
Recommended Usage

ELISA

0.5-2 ug/ml

Western Blot

0.5-2 ug/ml
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Caspase-8

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-8 (FLICE) forms a direct link between the activation of CD95 and the caspase pathway. Overexpression of Caspase-8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family.

Alternate Names

CASP8, Caspase8, Mch5

Gene Symbol

CASP8

Additional Caspase-8 Products

Product Documents for Caspase-8 Antibody (90A992) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Caspase-8 Antibody (90A992) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Caspase-8 Antibody (90A992) [Biotin]

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FAQs for Caspase-8 Antibody (90A992) [Biotin]

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  • Q: May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?

    A: In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail