Caspase-8 Antibody (90A992) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80616

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Simple Western, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 90A992

Format

Azide and BSA Free
View all Caspase-8 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product falls between amino acids 360-385 of the human Caspase 8 protein.

Reactivity Notes

Chimpanzee and Rhesus Monkey reactivity noted.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Caspase-8 Antibody (90A992) - Azide and BSA Free

Western Blot: Caspase-8 Antibody (90A992)Azide and BSA Free [NBP2-80616]

Western Blot: Caspase-8 Antibody (90A992)Azide and BSA Free [NBP2-80616]

Western Blot: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616] - Western blot analysis of Caspase-8 in Jurkat cells using Caspase-8 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-8 activation is detected in western blots by the presence of Caspase-8 cleavage fragments. The antibody detected both pro (full length) and active (cleaved) Caspase-8, depending on the treatment time points. A basal level of endogenously cleaved Caspase-8 can be see in untreated Jurkat cells. Goat anti-mouse lg HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616]

Immunohistochemistry-Paraffin: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616]

Immunohistochemistry-Paraffin: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616] - Formalin-fixed, paraffin-embedded human spleen (top) and breast (bottom) stained with Caspase-8 antibody at 4 ug/ml. Localization can be cytoplasmic and nuclear. Cancer/normal adjacent tissue array was used for this test. Staining of formalin-fixed tissue
Flow Cytometry: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616]

Flow Cytometry: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616]

Flow Cytometry: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616] - Flow cytometric analysis of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody was used for this test. IC-Flow (Intracellular Staining Flow Cytometry Kit) was used to fix and prepare the cells for staining. Image from the standard format of this antibody.
Western Blot: Caspase-8 Antibody (90A992)Azide and BSA Free [NBP2-80616]

Western Blot: Caspase-8 Antibody (90A992)Azide and BSA Free [NBP2-80616]

Western Blot: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616] - Analysis using the Biotin conjugate of NB100-56527. Detection of human Caspase-8 using Jurkat lysates with NB100-55786 at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2) dilution. NB100-55786 only detects 55 kDa Caspase-8 in Jurkat cells.
Western Blot: Caspase-8 Antibody (90A992)Azide and BSA Free [NBP2-80616]

Western Blot: Caspase-8 Antibody (90A992)Azide and BSA Free [NBP2-80616]

Western Blot: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616] - Caspase-8 expression in 786-O and A-498 cellsClose Image from the standard format of this antibody.
Simple Western: Caspase-8 Antibody (90A992)Azide and BSA Free [NBP2-80616]

Simple Western: Caspase-8 Antibody (90A992)Azide and BSA Free [NBP2-80616]

Simple Western: Caspase-8 Antibody (90A992) - Azide and BSA Free [NBP2-80616] - Simple Western lane view shows a specific band for Caspase 8 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.

Applications for Caspase-8 Antibody (90A992) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.1-0.5 ug/ml

Immunohistochemistry

reported in scientific literature (PMID 31034097)

Immunohistochemistry-Paraffin

4 ug/ml

Simple Western

1:100

Western Blot

0.5-2 ug/ml
Application Notes
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:100. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-8

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-8 (FLICE) forms a direct link between the activation of CD95 and the caspase pathway. Overexpression of Caspase-8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family.

Alternate Names

CASP8, Caspase8, Mch5

Gene Symbol

CASP8

Additional Caspase-8 Products

Product Documents for Caspase-8 Antibody (90A992) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Caspase-8 Antibody (90A992) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Caspase-8 Antibody (90A992) - Azide and BSA Free

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  • Q: May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?

    A: In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

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Associated Pathways

Apoptosis Signaling Pathway
Apoptosis Signaling Pathway Thumbnail