CCL2/MCP1 Antibody (2D8) - BSA Free

Novus Biologicals | Catalog # NBP2-22115

Novus Biologicals

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Feline, Primate

Cited:

Human, Mouse, Rat, Feline

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC, Knockdown Validated, Surface Plasmon Resonance

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2D8

Format

BSA Free
View all CCL2/MCP1 Primary Antibodies »

Product Specifications

Immunogen

Full lenght human MCP1 expressed in E. coli. [Uniprot: P13500]

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 29286133).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse CCL2/MCP1 Antibody (2D8) - BSA Free (NBP2-22115) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-CCL2/MCP1 Antibody: Cited in 27 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CCL2/MCP1 Antibody (2D8) - BSA Free

Western Blot: CCL2/MCP1 Antibody (2D8)BSA Free [NBP2-22115]

Western Blot: CCL2/MCP1 Antibody (2D8)BSA Free [NBP2-22115]

Western Blot: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] - Analysis using MCP1 mouse mAb against A549 (1), HeLa (2), Raw264.7 (3), L1210 (4), C6 (5), and COS-7 (6) cell lysates.
Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115]

Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115]

Immunocytochemistry/Immunofluorescence: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] - Analysis of HepG2 cells using A) MCP1 mAb (green), DRAQ5 fluorescent DNA dye (blue), and Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red). B) Staining without MCP1 mAb
Immunohistochemistry: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115]

Immunohistochemistry: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115]

CCL2-MCP1-Antibody-2D8-BSA-Free-Immunohistochemistry-NBP2-22115-img0009.jpg
Flow Cytometry: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115]

Flow Cytometry: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115]

Flow Cytometry: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] - Analysis of A549 cells using MCP1 mouse mAb (green) and negative control (red).
Western Blot: CCL2/MCP1 Antibody (2D8)BSA Free [NBP2-22115]

Western Blot: CCL2/MCP1 Antibody (2D8)BSA Free [NBP2-22115]

Western Blot: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] - Analysis using MCP1 mAb against human MCP1 (AA: 1-99) recombinant protein. (Expected MW is 36.5 kDa)
Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115]

Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115]

CCL2-MCP1-Antibody-2D8-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-22115-img0010.jpg
Immunohistochemistry: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115]

Immunohistochemistry: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115]

CCL2-MCP1-Antibody-2D8-BSA-Free-Immunohistochemistry-NBP2-22115-img0012.jpg
Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115]

Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115]

Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] - Analysis of paraffin-embedded liver cancer tissues using MCP1 mouse mAb with DAB staining.
ELISA: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115]

ELISA: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115]

ELISA: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).
CCL2/MCP1 Antibody (2D8) - BSA Free

Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] -

Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] - Expression patterns of CCL2 & CCR2 in unwounded & wounded corneas of WT & MMP12 KO mice. (A) Immunofluorescence of CCL2 chemokine & (B) its receptor CCR2 in unwounded & chemically wounded (2-days after injury) WT & Mmp12−/− mouse corneas. Control images represent mouse corneas stained with secondary antibody only & without primary antibody. Nuclei were visualized by staining with DAPI (blue). Scale bars: 50 µm. A magnified image of a wounded WT cornea shows perinuclear expression of CCL2 (orange box). CCL2 staining was visualized in epithelial, stromal, & endothelial layers of wounded WT & Mmp12−/− corneas. CCR2 staining was visualized in epithelial & stromal layers of unwounded & wounded WT & Mmp12−/− corneas. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31399604), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CCL2/MCP1 Antibody (2D8) - BSA Free

Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] -

Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] - LIF represses CXCL9 through epigenetic silencing. a, b qRT-PCR analysis of the indicated genes in BMDMs. BMDMs were pre-incubated with 20 ng/ml LIF for 72 h & then stimulated with 5 ng/ml IFN gamma or 10 μg/ml IL4 during 6 h (a) or with 0.1, 0.5, 1 & 5 ng/ml IFN gamma for 24 h (b). c CXCL9 ELISA from BMDMs pre-incubated with 20 ng/ml LIF & then stimulated with 0.1 ng/ml IFN gamma for 24 h. d CXCL9 ELISA from human CD11b+sorted cells (77% CD11b+ CD14+, see Supplementary Fig. 7b, c) from human GBM cultured with 20 ng/ml LIF for 72 h & then with 0.1 ng/ml IFN gamma for 24 h. e ChIP of Tri-methyl-histone H3 (H3K27me3), EZH2 & acetyl-histone4 (H4ac) was performed in BMDMs treated with 20 ng/ml LIF for 72 h. Scheme shows the analysed CXCL9 promoter region. Representative data are presented as mean ± SD. f Representative images of IF of the indicated markers in human GBM organotypic slices (patients 1, 2, 3) incubated with 10 μg/ml anti-LIF for 3 days. Scale bar, 20 μm. (see Supplementary Fig. 8). Bottom, percentage of double positive cells relative to Iba1+ cells & percentage of CXCL9+ cells relative to the total number of cells. Data are mean of all patients ± SEM. Statistical analyses by Student’s t-test or Mann–Whitney T-test. *P < 0.05, **P < 0.01; ***P < 0.001; ****P < 0.0001 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31186412), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CCL2/MCP1 Antibody (2D8) - BSA Free

Immunohistochemistry: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] -

Immunohistochemistry: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] - LIF regulates CXCL9, CCL2, CD206, & CD163 in TAMs. a Differential expression analysis of isolated CD11b+ cells from anti-LIF treated ID8 mice vs. control. Volcano plot representing the genes significantly (Q-value < 0.1) overexpressed (brown) & significantly underexpressed (turquoise). Heatmap representing the expression values of the indicated genes, each column represents a sample & each row a gene. The last column represents the log2 fold change (log2 FC) of gene expression. b mRNA expression for the indicated genes in isolated CD11b+ cells from anti-LIF treated or untreated ID8 & GL261N tumors. c Percentage & mean fluorescence intensity (MFI) of CCL2 & CXCL9 in TAMs (CD11b+ Ly6G− Ly6C−) from anti-LIF treated or untreated GL261N tumors. d Representative IF images of Iba1 & the indicated markers stainings of GL261N tumors (see Supplementary Fig. 4). Scale bar, 20 μm. Bottom, percentage of double positive cells relative to the TAM marker positive cells. CXCL9 quantification is relative to the total number of cells. e Tumor growth of GL261N in WT, CXCL9−/−, & CCL2−/− mice or mice treated with the indicated antibodies is shown as total flux (p/s). f Fold change (FC) of tumor infiltrating CD8+ T cells in the indicated treatments. Data are mean ± SEM. g, h Representative IHC of the indicated markers from 20 GBM tumors. The degree of staining was quantified using H-score method. Correlations between LIF & CCL2, CD206, CD163, & CXCL9 with the R-squared coefficients (R2) were calculated (h). Statistical analyses by Mann–Whitney T-test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31186412), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CCL2/MCP1 Antibody (2D8) - BSA Free

Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] -

Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] - LIF regulates CXCL9, CCL2, CD206, & CD163 in TAMs. a Differential expression analysis of isolated CD11b+ cells from anti-LIF treated ID8 mice vs. control. Volcano plot representing the genes significantly (Q-value < 0.1) overexpressed (brown) & significantly underexpressed (turquoise). Heatmap representing the expression values of the indicated genes, each column represents a sample & each row a gene. The last column represents the log2 fold change (log2 FC) of gene expression. b mRNA expression for the indicated genes in isolated CD11b+ cells from anti-LIF treated or untreated ID8 & GL261N tumors. c Percentage & mean fluorescence intensity (MFI) of CCL2 & CXCL9 in TAMs (CD11b+ Ly6G− Ly6C−) from anti-LIF treated or untreated GL261N tumors. d Representative IF images of Iba1 & the indicated markers stainings of GL261N tumors (see Supplementary Fig. 4). Scale bar, 20 μm. Bottom, percentage of double positive cells relative to the TAM marker positive cells. CXCL9 quantification is relative to the total number of cells. e Tumor growth of GL261N in WT, CXCL9−/−, & CCL2−/− mice or mice treated with the indicated antibodies is shown as total flux (p/s). f Fold change (FC) of tumor infiltrating CD8+ T cells in the indicated treatments. Data are mean ± SEM. g, h Representative IHC of the indicated markers from 20 GBM tumors. The degree of staining was quantified using H-score method. Correlations between LIF & CCL2, CD206, CD163, & CXCL9 with the R-squared coefficients (R2) were calculated (h). Statistical analyses by Mann–Whitney T-test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31186412), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CCL2/MCP1 Antibody (2D8) - BSA Free

Western Blot: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] -

The production of MCP-1, VCAM-1, and ICAM-1 was significantly inhibited in Kaempferol treatment during sepsis(A) The protein expression level of MCP-1, VCAM-1, and ICAM-1 at 24 h; (B) protein expression level of MCP-1, VCAM-1, and ICAM-1 at 48 h in sham, saline-treated CLP mice, and kaempferol treated CLP mice (n = 3 per group, mean +/- S.E.M, *P<0.05, **P<0.01, ***P<0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37440431), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CCL2/MCP1 Antibody (2D8) - BSA Free

Western Blot: CCL2/MCP1 Antibody (2D8) - BSA Free [NBP2-22115] -

The production of MCP-1, VCAM-1, and ICAM-1 was significantly inhibited in Kaempferol treatment during sepsis(A) The protein expression level of MCP-1, VCAM-1, and ICAM-1 at 24 h; (B) protein expression level of MCP-1, VCAM-1, and ICAM-1 at 48 h in sham, saline-treated CLP mice, and kaempferol treated CLP mice (n = 3 per group, mean +/- S.E.M, *P<0.05, **P<0.01, ***P<0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37440431), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CCL2/MCP1 Antibody (2D8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1 - 3 ug/ml

Reviewed Applications

Read 1 review rated 3 using NBP2-22115 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCL2/MCP1

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

Alternate Names

C-C motif chemokine 2, chemokine (C-C motif) ligand 2, GDCF-2, HC11Monocyte secretory protein JE, MCAFMonocyte chemotactic and activating factor, MCP1 Monocyte chemotactic protein 1, MCP-1 Small-inducible cytokine A2, MGC9434, Monocyte chemoattractant protein 1, monocyte chemoattractant protein-1, SCYA2HSMCR30, small inducible cytokine A2 (monocyte chemotactic protein 1, homologous tomouse Sig-je), small inducible cytokine subfamily A (Cys-Cys), member 2, SMC-CF

Entrez Gene IDs

6347 (Human)

Gene Symbol

CCL2

UniProt

P13500

Additional CCL2/MCP1 Products

Product Documents for CCL2/MCP1 Antibody (2D8) - BSA Free

Certificate of Analysis

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Product Specific Notices for CCL2/MCP1 Antibody (2D8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CCL2/MCP1 Antibody (2D8) - BSA Free

Customer Reviews for CCL2/MCP1 Antibody (2D8) - BSA Free (1)

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  • CCL2/MCP1 Antibody (2D8)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: HIV-infected Rhesus macaque adult lung
    Species: Rhesus Macaque
    Verified Customer | Posted 11/25/2019
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Heat-mediated Antigen Retrieval with 10mM Na Citrate buffer, pH6.0. Block with 5% Normal Goat Serum in PBS for 30min, RT Primary antibody (MCP-1, 1:500 dilution) overnight, 4 C HRP-conjugated 2nd antibody (1:500) for 30min, RT DAB staining Countersigning with hematoxylin
    CCL2/MCP1 Antibody (2D8) - BSA Free NBP2-22115

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Protocols

View specific protocols for CCL2/MCP1 Antibody (2D8) - BSA Free (NBP2-22115):

Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes (keep slides in the sodium citrate buffer all the time).

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in PBS for 5 minutes.
3. Block each section with 100-400 ul blocking solution (1% BSA in PBS) for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul HRP polymer conjugated secondary antibody. Incubate 30 minutes at room temperature.
7. Wash sections three times in wash buffer for 5 minutes each.
8. Add 100-400 ul DAB substrate to each section and monitor staining closely.
9. As soon as the sections develop, immerse slides in deionized water.
10. Counterstain sections in hematoxylin.
11. Wash sections in deionized water two times for 5 minutes each.
12. Dehydrate sections.
13. Mount coverslips.


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