CD117/c-kit Antibody (C117/370)

Name: CD117/c-kit Antibody (C117/370)
Brand: Novus Biologicals
SKU: NBP2-29423
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-29423

Submit a review

Citations (1)

Product Documents

View Available Conjugates View Available Formulations

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # C117/370

View all CD117/c-kit Primary Antibodies »

Product Specifications

Immunogen

Recombinant human CD117/c-kit protein (Uniprot: P10721)

Localization

Cell Surface and Cytoplasm

Marker

Marker for Gastrointestinal Stromal Tumors

Specificity

This monoclonal antibody recognizes a protein of 145kDa, identified as CD117/p145kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117 has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi's sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117 is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Theoretical MW

145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33192)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD117/c-kit Antibody (C117/370)

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (C117/370) [NBP2-29423]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (C117/370) [NBP2-29423] - Formalin-fixed, paraffin-embedded human GIST stained with CD117/c-kit Mouse Monoclonal Antibody (C117/370).

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (C117/370) [NBP2-29423]

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (C117/370) [NBP2-29423] - Formalin-fixed, paraffin-embedded human GIST stained with CD117/c-kit Antibody (C117/370).

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (C117/370) [NBP2-29423] -

Immunohistochemistry-Paraffin: CD117/c-kit Antibody (C117/370) [NBP2-29423] - Formalin-paraffin human gastrointestinal stromal tumor(GIST) stained with CD117 Ab (C117/370).

Applications for CD117/c-kit Antibody (C117/370)

Application

Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Application Notes

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 9.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD117/c-kit

CD117 is also know as c-Kit. Antibodies to CD117 / c-Kit may be useful for the identification of several cancers including mast cell diseases, acute myeloid leukemia (AML), small cell lung carcinoma (SCLC), and Ewing's sarcoma, and in the differentiation between gastrointestinal stromal tumors (GISTs) and other intra-abdominal mesenchymal tumors

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Gene Symbol

KIT

UniProt

P10721

Additional CD117/c-kit Products

Product Documents for CD117/c-kit Antibody (C117/370)

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD117/c-kit Antibody (C117/370)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.