CD11c Antibody (CL1831) - BSA Free

Novus Biologicals | Catalog # NBP2-34491

Novus Biologicals

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1831

Format

BSA Free
View all CD11c Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P20702, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Marker

Dendritic Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse CD11c Antibody (CL1831) - BSA Free (NBP2-34491) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD11c Antibody (CL1831) - BSA Free

CD11c Antibody (CL1831) - BSA Free CD11c Antibody (CL1831)

Detection of CD11c in Mouse Spleen via seqIF™ staining on COMET™​

CD11c was detected in immersion fixed paraffin-embedded sections of mouse Spleen using Mouse anti-CD11c, Monoclonal Antibody (Catalog #NBP2-34491) at 1:200 dilution at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining in human tonsil and kidney tissues. Corresponding ITGAX RNA-seq data are presented for the same tissues.
Western Blot: CD11c Antibody (CL1831) [NBP2-34491]

Western Blot: CD11c Antibody (CL1831) [NBP2-34491]

Western Blot: CD11c Antibody (CL1831) [NBP2-34491] - Lane 1: Marker [kDa]. Lane 2: Human tonsil.
Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - CD11c Positive cells in mouse liver tissue. Primary antibody dilution: 1:100. Citrate buffer antigen retrieval. Overnight incubation RT. Image from verified customer review.
Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining of human tonsil shows strong membranous positivity in a subset of germinal and non-germinal center cells.
Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining of human small intestine shows moderate positivity in a subset of lymphoid cells.
Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining of human fallopian tube shows moderate membranous positivity in a subset of lymphoid cells.
Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491]

Immunohistochemistry-Paraffin: CD11c Antibody (CL1831) [NBP2-34491] - Staining of human kidney shows no positivity as expected.

Applications for CD11c Antibody (CL1831) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 4 using NBP2-34491 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD11c

CD11c encodes the integrin alpha X chain protein. CD11c is a type I transmembrane protein found at high levels on most human dendritic cells, but also on monocytes, macrophages, neutrophils, and some B cells that induces cellular activation and helps trigger neutrophil respiratory burst; expressed in hairy cell leukemias, acute nonlymphocytic leukemias, and some B-cell chronic lymphocytic leukemias.

Alternate Names

CD11c, Integrin alpha X, ITGAX, p150,95 alpha

Gene Symbol

ITGAX

UniProt

P20702 (Human)

Additional CD11c Products

Product Documents for CD11c Antibody (CL1831) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD11c Antibody (CL1831) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CD11c Antibody (CL1831) - BSA Free (1)

4 out of 5
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  • CD11c Antibody (CL1831)
    Name: Mozhdeh Sojoodi
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Liver and Liver tissue
    Species: Mouse
    Verified Customer | Posted 02/07/2022
    Cd11c Positive cells in mouse liver
    1:100 dilution Citrate buffer antigen retrieval Overnight incubation RT
    CD11c Antibody (CL1831) - BSA Free NBP2-34491
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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FAQs

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