CD133 Antibody - BSA Free
Novus Biologicals | Catalog # NB120-16518
![Western Blot: CD133 AntibodyBSA Free [NB120-16518]](https://beta-resources.rndsystems.com/images/products/CD133-Antibody---BSA-Free-Western-Blot-NB120-16518-img0003.jpg "Western Blot: CD133 AntibodyBSA Free [NB120-16518]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine
Cited:
Human, Mouse, Rat, Porcine
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Synthetic peptide corresponding to a C-terminal region of CD133 (within amino acids 750-865).
Reactivity Notes
porcine reactivity reported in scientific literature (PMID: 29352176).
Marker
Stem Cell Marker
Specificity
CD133 - Hematopoietic Stem Cell Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit CD133 Antibody - BSA Free (NB120-16518) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow, ICC/IF and ChIP. Anti-CD133 Antibody: Cited in 41 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CD133 Antibody - BSA Free
Western Blot: CD133 AntibodyBSA Free [NB120-16518]
Western Blot: CD133 Antibody - BSA Free [NB120-16518] - Predicted band size : 97 kDa recognizes 97 kDa human CD133 in Y79 cells. Band is at ~120 kDa due to protein glycosylation.![Immunocytochemistry/ Immunofluorescence: CD133 Antibody - BSA Free [NB120-16518]](https://beta-resources.rndsystems.com/images/products/CD133 Antibody - BSA Free-Immunocytochemistry-Immunofluorescence-NB120-16518-img0008.jpg "Immunocytochemistry/ Immunofluorescence: CD133 Antibody - BSA Free [NB120-16518]")
Immunocytochemistry/ Immunofluorescence: CD133 Antibody - BSA Free [NB120-16518]
CD133 Antibody - BSA Free-Immunocytochemistry-Immunofluorescence-NB120-16518-img0008.jpg![Immunohistochemistry: CD133 Antibody - BSA Free [NB120-16518]](https://beta-resources.rndsystems.com/images/products/CD133-Antibody---BSA-Free-Immunohistochemistry-NB120-16518-img0007.jpg "Immunohistochemistry: CD133 Antibody - BSA Free [NB120-16518]")
Immunohistochemistry: CD133 Antibody - BSA Free [NB120-16518]
CD133-Antibody---BSA-Free-Immunohistochemistry-NB120-16518-img0007.jpg![Flow Cytometry: CD133 Antibody - BSA Free [NB120-16518]](https://beta-resources.rndsystems.com/images/products/CD133-Antibody---BSA-Free-Flow-Cytometry-NB120-16518-img0006.jpg "Flow Cytometry: CD133 Antibody - BSA Free [NB120-16518]")
Flow Cytometry: CD133 Antibody - BSA Free [NB120-16518]
Flow Cytometry: CD133 Antibody - BSA Free [NB120-16518] - Flow Cytometry: CD133 Antibody [DyLight 488] [NB120-16518G] - Flow Cytometry: CD133 Antibody (DyLight488) [NB120-16518G] - Rat bone marrow cells were stained with CD133 (1:100) antibody (20 minutes at 4C), fixed, and then analyzed. Image using the DyLight 488 form of this antibody.
Chromatin Immunoprecipitation: CD133 Antibody - BSA Free [NB120-16518] - [NB 120-16518] - Staining CD133 in Bone Marrow Mononuclear Cells by Immunofluorescence.
![Immunohistochemistry-Paraffin: CD133 Antibody - BSA Free [NB120-16518]](https://beta-resources.rndsystems.com/images/products/CD133-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB120-16518-img0002.jpg "Immunohistochemistry-Paraffin: CD133 Antibody - BSA Free [NB120-16518]")
Immunohistochemistry-Paraffin: CD133 Antibody - BSA Free [NB120-16518]
Immunohistochemistry-Paraffin: CD133 Antibody - BSA Free [NB120-16518] - Staining of CD133 in human hepatocarcinoma (HC) tissue. NB120-16518 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.Applications for CD133 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:1000
Flow Cytometry
1:10-1:1000
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:100-1:250
Immunohistochemistry-Paraffin
1:100-1:250
Western Blot
1:500-1:1000
Application Notes
Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). IHC: Citrate buffer antigen retreival required. ICC/IF: Fix with 3% paraformaldehyde for 20 min at RT. Optimal dilutions/concentrations should be determined by the end user. Use in IHC-Frozen reported in scientific literature (PMID: 29352176).
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD133
Alternate Names
AC133, CD133, PROM1, Prominin 1, PROML1
Gene Symbol
PROM1
Additional CD133 Products
Product Documents for CD133 Antibody - BSA Free
Product Specific Notices for CD133 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for CD133 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ChIP Protocol Video
- Chromatin Immunoprecipitation (ChIP) Protocol
- Chromatin Immunoprecipitation Protocol
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
