CD133 Antibody - BSA Free

Flow Cytometry: CD133 Antibody - BSA Free [NB120-16518]

Flow Cytometry: CD133 Antibody - BSA Free [NB120-16518] - Flow Cytometry: CD133 Antibody [DyLight 488] [NB120-16518G] - Flow Cytometry: CD133 Antibody (DyLight488) [NB120-16518G] - Rat bone marrow cells were stained with CD133 (1:100) antibody (20 minutes at 4C), fixed, and then analyzed. Image using the DyLight 488 form of this antibody.

Chromatin Immunoprecipitation: CD133 Antibody - BSA Free [NB120-16518] - [NB 120-16518] - Staining CD133 in Bone Marrow Mononuclear Cells by Immunofluorescence.

Immunohistochemistry-Paraffin: CD133 Antibody - BSA Free [NB120-16518]

Immunohistochemistry-Paraffin: CD133 Antibody - BSA Free [NB120-16518] - Staining of CD133 in human hepatocarcinoma (HC) tissue. NB120-16518 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

Western Blot: CD133 Antibody - BSA Free [NB120-16518] -

TRPM7 is expressed in GBM and positively correlates with the activation of Notch1, and the upregulation of TRPM7 activates Notch1 signaling. (A) The U87MG cells were transfected with (A) wild-type human TRPM7 (M7-wt); (B) two alpha -kinase-inactive mutants, “ alpha -kinase-dead" point mutation (K1648R, or M7-KR) and alpha -kinase deleted mutant (M7-DK) along with controls followed by assaying protein expression of TRPM7, Notch1, Notch2, Notch3, and Notch4 by Western blot. (B) HEK-293 cells were transfected with a pcDNA4/TO plasmid that allowed tetracycline-inducible protein expression of TRPM7-wt tagged with HA. Then, protein expression of exogenous, endogenous TRPM7, Notch1, Notch2, Notch3, Notch4, and Notch target genes Hey2 and Survivin were determined by Western blot. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33381038), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CD133 Antibody - BSA Free [NB120-16518] -

Immunostaining for CD133 in normal ovarian and tumor tissue sections: [A] Mouse monoclonal anti-CD133 antibody was localized in both OSE (A, B) and ovarian cortex (C, D) by immunohistochemistry. Region between dotted boxes in A, C is magnified in B, D respectively. Polar staining of CD133 is obvious in OSE layer especially in NO, BL and HG ovaries. BL ovaries exhibit multi-layered OSE. Cortex comprised of CD133+ cells arranged in clusters with elongated/spindle shaped morphology in NO and BN ovaries. BL ovarian cortex harbours single spherical cell clusters distributed throughout. HG comprised more of large CD133+ cells in OSE and few clusters in the cortex per field focussed. Insets include magnified images of cells from different fields. Scale bar = 100 μm (A, C) and 25 μm (B, D) respectively. [B] Immunofluorescence staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals specific CD133+ cells with relatively higher cell numbers in BL and HG. Area within dotted lines in BN OSE (A) are magnified in (B) while elliptical/spindle shaped CD133+ cells in cortex from various fields were represented in the composite image in (C) of BN and HG. Large CD133+ cells in cortex were also observed. White scale bar = 50 μm; blue scale bar = 10 μm. Secondary antibody employed was conjugated with Alexa fluor 568 and tissue sections were counterstained with nucleus specific dye DAPI Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30121075), licensed under a CC-BY license. Not internally tested by Novus Biologicals.