CD14 Antibody (SC69-02)

Western Blot: CD14 Antibody (SC69-02) [NBP2-67630]

Western Blot: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of CD14 on NIH/3T3 cell lysates with Rabbit anti-CD14 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 40 kDa Observed band size: 65 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: CD14 Antibody (SC69-02) [NBP2-67630]

Immunocytochemistry/Immunofluorescence: CD14 Antibody (SC69-02) [NBP2-67630] - Staining CD14 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630]

Immunohistochemistry-Paraffin: CD14 Antibody (SC69-02) [NBP2-67630] - Analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-CD14 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.