CD19 Antibody (CB19) - Azide and BSA Free

Name: CD19 Antibody (CB19) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-80634
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-80634

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, In vitro assay, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # CB19

Format

Azide and BSA Free

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Product Specifications

Immunogen

Intact normal human B cell cells were used as immunogen to generate the CB19 clone.

Epitope

The exact CD19 epitope recognized by the antibody has not been mapped and is unknown.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27532872).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

Novus Biologicals Mouse CD19 Antibody (CB19) - Azide and BSA Free (NBP2-25196) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD19 Antibody (CB19) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD19 Antibody (CB19) - Azide and BSA Free [NBP2-80634]

Immunocytochemistry/Immunofluorescence: CD19 Antibody (CB19) - Azide and BSA Free [NBP2-80634] - The CD19 (CB19) antibody was tested in Daudi cells at a 1:40 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively. Image from the standard format of this antibody.

Flow Cytometry: CD19 Antibody (CB19) - Azide and BSA Free [NBP2-80634]

Flow Cytometry: CD19 Antibody (CB19) - Azide and BSA Free [NBP2-80634] - Analysis using the PE conjugate of NBP2-25196. Staining of CD19 in 1x10^6 human PBMC using 10 ul (0.25 ug) of was used to test this product. Propidium iodide negative lymphocyte population gated for analysis.

Flow Cytometry: CD19 Antibody (CB19) - Azide and BSA Free [NBP2-80634]

Flow Cytometry: CD19 Antibody (CB19) - Azide and BSA Free [NBP2-80634] - A surface stain was performed on human peripheral blood lymphocytes with CD9 (HI9a) antibody NB100-77915AF647 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-26646. Image from the standard format of this antibody.

Flow Cytometry: CD19 Antibody (CB19) - Azide and BSA Free [NBP2-80634]

Flow Cytometry: CD19 Antibody (CB19) - Azide and BSA Free [NBP2-80634] - A surface stain was performed on human peripheral blood lymphocytes with TACI/TNFRSF13B/CVID (1A1) antibody NB600-1189PE and a matched isotype control. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25196AF647. Image from the standard format of this antibody.

Flow Cytometry: CD19 Antibody (CB19) - Azide and BSA Free [NBP2-80634]

Flow Cytometry: CD19 Antibody (CB19) - Azide and BSA Free [NBP2-80634] - A surface stain was performed on Ramos cells with CD19 Antibody (CB19) NBP2-26646 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to phycoerythrin.

Flow Cytometry: CD19 Antibody (CB19) - Azide and BSA Free [NBP2-80634]

Flow Cytometry: CD19 Antibody (CB19) - Azide and BSA Free [NBP2-80634] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25196. Staining of human PBMC. Image from verified customer review.

Flow Cytometry: CD19 Antibody (CB19) - Azide and BSA Free [NBP2-80634]

Flow Cytometry: CD19 Antibody (CB19) - Azide and BSA Free [NBP2-80634] - Analysis using the DyLight 405 conjugate of NBP2-25196. Staining of CD19 in human PBMCs using anti-CD19 antibody. Image from verified customer review.

Flow Cytometry: CD19 Antibody (CB19) - Azide and BSA Free [NBP2-80634]

Flow Cytometry: CD19 Antibody (CB19) - Azide and BSA Free [NBP2-80634] - Analysis using the FITC conjugate of NBP2-25196. Staining of CD19 in 10^6 human lymphocytes using 10 ul (0.25 ug) of this antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents anti-CD19 antibody.

Flow Cytometry: CD19 Antibody (CB19) - Azide and BSA Free [NBP2-80634]

Flow Cytometry: CD19 Antibody (CB19) - Azide and BSA Free [NBP2-80634] - Surface staining of 10^6 human lymphocytes using 0.2 ug of CD19 antibody (red) and isotype control (green). Cell surface staining kit was used for this test. Image from the standard format of this antibody.

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Applications for CD19 Antibody (CB19) - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

0.2 ug/10^6 cells

Immunocytochemistry/ Immunofluorescence

1:25-1:100

In vitro assay

reported in scientific literature (PMID 9576485)

Western Blot

reported in scientific literature (PMID 17327405)

Application Notes

This antibody is CyTOF ready.

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD19

CD19 (Cluster of Differentiation 19), also known as B-lymphocyte surface antigen B4, is a type 1 transmembrane glycoprotein belonging to immunoglobulin (Ig) subfamily that serves as a biomarker for normal and neoplastic B cells (1,2). CD19 is a co-receptor for the B cell receptor (BCR) signaling complex and has a critical role in regulating B cell signaling and immune response (1,2). The CD19 protein contains an extracellular N-terminus containing two C2 Ig-like domains separated by a helical non-Ig domain, a single pass transmembrane domain, and a highly conserved cytoplasmic C-terminal domain (1,2). The human CD19 protein, encoded by the CD19 gene located on chromosome 16p11.2, is 556 amino acids (aa) in length with a calculated theoretical molecular weight (MW) of 61 kDa and an observed molecular weight of 95 kDa (1-3). CD19 associates with other molecules - CD21, CD81, and CD225 - to form the BCR co-complex, also called the CD19 complex, through CD21 binding to the complement C3d complex (1-3). Complement C3d bridges the BCR with the CD19 complex into lipid rafts of the plasma membrane (1-3). CD19 is capable of modulating B cell development through both BCR-dependent and -independent signaling (1-3). Upon BCR activation, the tyrosine residues of CD19's cytoplasmic tail recruits multiple kinases including Lyn, Vav, and PI3K, amplifying BCR-mediated immune signaling and B cell activation (1-3).

Considering the role of CD19 in BCR signaling and its expression in development from pre-B cells through plasma cells, it is understandable that CD19 dysfunction and abnormal expression is associated with numerous B cell malignancies and autoimmune disorders (1-5). CD19 expression is typically observed at relatively normal levels in B cell acute lymphoblastic leukemia (B-ALL) and chronic lymphoblastic leukemia (CLL) but is often reduced other types of lymphoma including diffuse large B cell lymphoma (DLBCL) and follicular lymphoma (FL) (1,2). On the other hand, CD19 expression is typically increased in autoimmune disorders such as systemic sclerosis (SSc) and multiple sclerosis (MS) as modeled by experimental autoimmune encephalomyelitis (EAE) (2). CD19 has become a therapeutic molecular target for the treatment of B cell lymphomas and autoimmune disorders using monoclonal antibodies (mAbs), bi-specific T cell engaging (BiTE) antibodies, and CD19-specific chimeric antigen receptor (CAR) T cells (1,2,4-6). Although anti-CD19 CAR T cell therapy has become the standard for the treatment of B cell malignancies, patients may experience relapse due to resistance mechanisms (6). Strategies to improve efficacy and limit relapse include combination of CAR T cell therapy with immune checkpoint inhibitors like anti-PD-1 (4,6).

References

1. Wang K, Wei G, Liu D. CD19: a biomarker for B cell development, lymphoma diagnosis and therapy. Exp Hematol Oncol. 2012;1(1):36. https://doi.org/10.1186/2162-3619-1-36

2. Li X, Ding Y, Zi M, et al. CD19, from bench to bedside. Immunol Lett. 2017;183:86-95. https://doi.org/10.1016/j.imlet.2017.01.010

3. Wentink MWJ, van Zelm MC, van Dongen JJM, Warnatz K, van der Burg M. Deficiencies in the CD19 complex. Clin Immunol. 2018;195:82-87. https://doi.org/10.1016/j.clim.2018.07.017

4. Frigault MJ, Maus MV. State of the art in CAR T cell therapy for CD19+ B cell malignancies. J Clin Invest. 2020;130(4):1586-1594. https://doi.org/10.1172/JCI129208

5. Penack O, Koenecke C. Complications after CD19+ CAR T-Cell Therapy. Cancers (Basel). 2020;12(11):3445. https://doi.org/10.3390/cancers12113445

6. Bouziana S, Bouzianas D. Anti-CD19 CAR-T cells: Digging in the dark side of the golden therapy. Crit Rev Oncol Hematol. 2021;157:103096. https://doi.org/10.1016/j.critrevonc.2020.103096

Alternate Names

CD19, CVID3, Leu-12

Gene Symbol

CD19

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Product Specific Notices for CD19 Antibody (CB19) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.