CD20 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90051

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Equine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CD20 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSP

Reactivity Notes

Mouse and equine reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit CD20 Antibody - BSA Free (NBP1-90051) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD20 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CD20 Antibody [NBP1-90051]

Immunocytochemistry/ Immunofluorescence: CD20 Antibody [NBP1-90051]

Immunocytochemistry/Immunofluorescence: CD20 Antibody [NBP1-90051] - Staining of human cell line REH shows localization to nucleus & plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human cerebral cortex, lymph node, spleen and testis using Anti-MS4A1 antibody NBP1-90051 (A) shows similar protein distribution across tissues to independent antibody NBP1-90052 (B).
CD20 Antibody - BSA Free Immunohistochemistry: CD20 Antibody - BSA Free [NBP1-90051]

Immunohistochemistry: CD20 Antibody - BSA Free [NBP1-90051]

Analysis in human spleen and cerebral cortex tissues using NBP1-90051 antibody. Corresponding MS4A1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human spleen shows strong membranous positivity in cells in white pulp.
Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human spleen using Anti-MS4A1 antibody NBP1-90051.
Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human testis.
Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human gastrointestinal shows strong membranous positivity in lymphocytes.
Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human tonsil shows strong membranous positivity in germinal center cells.
Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human lymph node shows strong membranous positivity in germinal center cells.
Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human cerebral cortex using Anti-MS4A1 antibody NBP1-90051.
Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051]

Immunohistochemistry-Paraffin: CD20 Antibody [NBP1-90051] - Staining of human lymph node using Anti-MS4A1 antibody NBP1-90051.
CD20 Antibody - BSA Free Western Blot: CD20 Antibody - BSA Free [NBP1-90051]

Western Blot: CD20 Antibody - BSA Free [NBP1-90051]

Analysis using antibody NBP1-90051 (A) shows similar pattern to independent antibody NBP1-90052 (B).

Applications for CD20 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100

Reviewed Applications

Read 3 reviews rated 5 using NBP1-90051 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD20

CD20 is a non-glycosylated phosphoprotein that is expressed on the surface of normal and malignant B cells and functions in mediating calcium transport and B cell differentiation (1,2). CD20 is encoded by the membrane-spanning 4-domain family A member 1 (MS4A1) gene and, in humans, is located on chromosome 11q12 (1). The CD20 protein is 297 amino acids (aa) in length with a theoretical molecular weight (MW) of 33 kDa (1,2). Structurally, the CD20 protein has four membrane-spanning domains, two extracellular loop domains, and intracellular N- and C-terminal domains (1,2). CD20 is expressed at specific stages of B cell maturation including pre-B cells, mature naive and activated B cells, and memory B cells, but is absent from plasmablasts and plasma cells (1,3,4). Expression of CD20 is often increased on malignant B cells associated with various B cell disorders such as chronic lymphocytic leukemia (CLL), multiple sclerosis (MS), and rheumatoid arthritis (2-4). Anti-CD20 monoclonal antibody (mAb)-based therapies have become an appealing target for treating these immune-related disorders and cancers (1-5). Rituximab, a chimeric mAb, was the first FDA approved CD20 monoclonal antibody for the treatment of non-Hodgkin's lymphoma that is now commonly used to treat MS (2,3). Since its initial approval in 1997, several other chimeric and humanized anti-CD20 mAbs have been developed including Ofatumumab, Ublituximab, and Obinutuzumab (1-5). B cell depletion via CD20 mAbs can occur under different mechanisms such as antibody-dependent cellular cytotoxicity (ADCC), antibody-dependent cellular phagocytosis (ADCP), complement-dependent cytotoxicity (CDC), and direct induction of apoptosis (1-4). Many ongoing studies are focused on combination therapies with CD20 mAbs as an addition to chemotherapy, B cell receptor (BCR) signaling inhibitors, or BH3 mimetics (1,2,4). Additionally, the effects of bispecific antibodies and CD20 chimeric antigen receptor (CAR) T cell therapies are under investigation for the treatment of B cell malignancies (4,5).

References

1. Pavlasova G, Mraz M. The regulation and function of CD20: an "enigma" of B-cell biology and targeted therapy. Haematologica. 2020; 105(6):1494-1506. https://doi.org/10.3324/haematol.2019.243543

2. Payandeh Z, Bahrami AA, Hoseinpoor R, et al. The applications of anti-CD20 antibodies to treat various B cells disorders. Biomed Pharmacother. 2019; 109:2415-2426. https://doi.org/10.1016/j.biopha.2018.11.121

3. Margoni M, Preziosa P, Filippi M, Rocca MA. Anti-CD20 therapies for multiple sclerosis: current status and future perspectives. J Neurol. 2022; 269(3):1316-1334. https://doi.org/10.1007/s00415-021-10744-x

4. Klein C, Jamois C, Nielsen T. Anti-CD20 treatment for B-cell malignancies: current status and future directions. Expert Opin Biol Ther. 2021; 21(2):161-181. https://doi.org/10.1080/14712598.2020.1822318

5. Sharman JP. Targeting CD20: teaching an old dog new tricks. Hematology Am Soc Hematol Educ Program. 2019; 2019(1):273-278. https://doi.org/10.1182/hematology.2019000031

Long Name

Cluster of Differentiation 20

Alternate Names

B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7

Gene Symbol

MS4A1

Additional CD20 Products

Product Documents for CD20 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD20 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CD20 Antibody - BSA Free (3)

5 out of 5
3 Customer Ratings
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Showing  1 - 3 of 3 reviews Showing All
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  • CD20 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Spleen
    Species: Mouse
    Verified Customer | Posted 05/08/2024
    CD20 immunoreactivity in an FFPE section of mouse spleen. NBP1 90051 was diluted to 250ng per mL and was left on tissue sections for 30m at room temperature. Secondary was horse anti rabbit HRP polymer.
    Section underwent heat-induced epitope retrieval for 20m in a vegetable steamer using Target Retrieval Solution.
    CD20 Antibody - BSA Free NBP1-90051
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • CD20 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Tonsil
    Species: Human
    Verified Customer | Posted 05/07/2024
    CD20 immunoreactivity in an FFPE section of human tonsil. NBP1-90051 was diluted to 250ng per mL and was left on tissue sections for 30m at room temperature. Secondary was horse anti rabbit HRP polymer.
    Section underwent heat induced epitope retrieval in a vegetable steamer for 20m using Target Retrieval Solution.
    CD20 Antibody - BSA Free NBP1-90051
  • CD20 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Spleen
    Species: Horse
    Verified Customer | Posted 05/07/2024
    CD20 immunoreactivity in an FFPE section of horse spleen. NBP1-90051 was diluted to 250ng per mL and was left on tissue sections for 30m at room temperature. Secondary was horse anti rabbit HRP polymer.
    Section underwent heat induced epitope retrieval for 20min in a vegetable steamer using Target Retrieval Solution.
    CD20 Antibody - BSA Free NBP1-90051
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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