CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free

Novus Biologicals | Catalog # NB100-64796

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Human, Mouse, Rat, Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TLD-3A12

Format

BSA Free
View all CD31/PECAM-1 Primary Antibodies »

Product Specifications

Immunogen

Activated, Lewis rat derived microglial cells

Reactivity Notes

Predicted cross-reactivities: Rhesus Monkey, Porcine

Localization

Membrane; single pass type I membrane protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

82.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free [NB100-64796]

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free [NB100-64796]

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (TLD-3A12) [NB100-64796] - Analysis using the FITC conjugate of CD31/PECAM-1 Antibody (TLD-3A12) (NB100-64796). Staining of rat brain cryosection with Mouse.
Flow Cytometry: CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free [NB100-64796]

Flow Cytometry: CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free [NB100-64796]

Flow Cytometry: CD31/PECAM-1 Antibody (TLD-3A12) [NB100-64796] - Analysis using the Alexa Fluor (R) 488 conjugate of CD31/PECAM-1 Antibody (TLD-3A12) (NB100-64796). Staining of PBMC's (Monocyte gate at 1 x 10^6 cells/ml) with CD31 antibody (orange) stained at a dilution of 1:500. Shown with mIgG (AF488) isotype control (blue).
Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free [NB100-64796]

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free [NB100-64796]

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (TLD-3A12) [NB100-64796] - Analysis of CD31 in 4th day cutaneous wound section of diabetic rat using CD31/PECAM-1 Antibody (TLD-3A12). Image from verified customer review.
Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free [NB100-64796]

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free [NB100-64796]

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (TLD-3A12) [NB100-64796] - Analysis using the FITC conjugate of CD31/PECAM-1 Antibody (TLD-3A12) (NB100-64796). Staining of rat spleen cryosection.
Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free [NB100-64796]

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free [NB100-64796]

CD31-PECAM-1-Antibody-TLD-3A12-Immunohistochemistry-Paraffin-NB100-64796-img0014.jpg
CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free

Immunohistochemistry: CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free [NB100-64796] -

(a) HE staining for wound tissues of different groups of rats (magnification ×40). (b) IHC analysis for the expression of CD31 of wound tissues of different groups of rats (magnification ×200). n = 6, ∗∗P < 0.01.

Applications for CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:100

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:100

Immunohistochemistry-Paraffin

1:10-1:500

Reviewed Applications

Read 1 review rated 5 using NB100-64796 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD31/PECAM-1

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1, TLD-3A12 CD31, TLD-3A12 Clone

Gene Symbol

PECAM1

UniProt

Q3SWT0 (Rat)

Additional CD31/PECAM-1 Products

Product Documents for CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free

Customer Reviews for CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free (1)

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Customer Images

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  • Name: Vinay Kant
    Application: Immunocytochemistry
    Sample Tested:
    Species: Rat
    Verified Customer | Posted 05/29/2015
    Immunohistochemistry for CD31 in 14th day cutaneous wound section of diabetic rat.
    CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free NB100-64796

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Protocols

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FAQs for CD31/PECAM-1 Antibody (TLD-3A12) - BSA Free

Showing  1 - 5 of 5 FAQs Showing All

  • Q: Can you give me more information about this antibody?

    A: Our mouse monoclonal antibody CD31/PECAM-1 Antibody (TLD-3A12) NB100-64796 was raised against activated Lewis rat derived microglial cells. Since we do not routinely epitope map our antibodies however, I am unable to tell you exactly where on the protein it binds.

  • Q: I potentially am interested in CD31/PECAM1 Antibody (TLD-3A12). I am woundering if this AB is suitable to use for immunohistochemistry with PFA-fixed section. Is it suitable?

    A: Yes, this antibody is suitable for IHC with PFA

  • Q: What is the concentration NB100-64796?

    A: The volume of the antibody provided is 250ul. The concentration of the antibody is 1.0mg/ml.

  • Q: What is the size of the target protein CD31/PECAM for this antibody?

    A: In western blot, we see bands between 115-160kDa for this particular target. This weight varies from the theoretical molecular weight of 76kDa due to glycosylation of the protein.

  • Q: Would this antibody apply to tissue that fixed after the formalin? 

    A: Formalin fixation should be acceptable for use with this antibody. We incubate freshly dissected tissue in 10% formalin for 4-24 hours, although the incubation time is dependent on tissue type and size. We do not recommend fixation for longer than 24 hours since this can cause epitope masking.

  • Q: Can you give me more information about this antibody?

    A: Our mouse monoclonal antibody CD31/PECAM-1 Antibody (TLD-3A12) NB100-64796 was raised against activated Lewis rat derived microglial cells. Since we do not routinely epitope map our antibodies however, I am unable to tell you exactly where on the protein it binds.

  • Q: I potentially am interested in CD31/PECAM1 Antibody (TLD-3A12). I am woundering if this AB is suitable to use for immunohistochemistry with PFA-fixed section. Is it suitable?

    A: Yes, this antibody is suitable for IHC with PFA

  • Q: What is the concentration NB100-64796?

    A: The volume of the antibody provided is 250ul. The concentration of the antibody is 1.0mg/ml.

  • Q: What is the size of the target protein CD31/PECAM for this antibody?

    A: In western blot, we see bands between 115-160kDa for this particular target. This weight varies from the theoretical molecular weight of 76kDa due to glycosylation of the protein.

  • Q: Would this antibody apply to tissue that fixed after the formalin? 

    A: Formalin fixation should be acceptable for use with this antibody. We incubate freshly dissected tissue in 10% formalin for 4-24 hours, although the incubation time is dependent on tissue type and size. We do not recommend fixation for longer than 24 hours since this can cause epitope masking.

  • Q: Can you give me more information about this antibody?

    A: Our mouse monoclonal antibody CD31/PECAM-1 Antibody (TLD-3A12) NB100-64796 was raised against activated Lewis rat derived microglial cells. Since we do not routinely epitope map our antibodies however, I am unable to tell you exactly where on the protein it binds.

  • Q: I potentially am interested in CD31/PECAM1 Antibody (TLD-3A12). I am woundering if this AB is suitable to use for immunohistochemistry with PFA-fixed section. Is it suitable?

    A: Yes, this antibody is suitable for IHC with PFA

  • Q: What is the concentration NB100-64796?

    A: The volume of the antibody provided is 250ul. The concentration of the antibody is 1.0mg/ml.

  • Q: What is the size of the target protein CD31/PECAM for this antibody?

    A: In western blot, we see bands between 115-160kDa for this particular target. This weight varies from the theoretical molecular weight of 76kDa due to glycosylation of the protein.

  • Q: Would this antibody apply to tissue that fixed after the formalin? 

    A: Formalin fixation should be acceptable for use with this antibody. We incubate freshly dissected tissue in 10% formalin for 4-24 hours, although the incubation time is dependent on tissue type and size. We do not recommend fixation for longer than 24 hours since this can cause epitope masking.

  • Q: Can you give me more information about this antibody?

    A: Our mouse monoclonal antibody CD31/PECAM-1 Antibody (TLD-3A12) NB100-64796 was raised against activated Lewis rat derived microglial cells. Since we do not routinely epitope map our antibodies however, I am unable to tell you exactly where on the protein it binds.

  • Q: I potentially am interested in CD31/PECAM1 Antibody (TLD-3A12). I am woundering if this AB is suitable to use for immunohistochemistry with PFA-fixed section. Is it suitable?

    A: Yes, this antibody is suitable for IHC with PFA

  • Q: What is the concentration NB100-64796?

    A: The volume of the antibody provided is 250ul. The concentration of the antibody is 1.0mg/ml.

  • Q: What is the size of the target protein CD31/PECAM for this antibody?

    A: In western blot, we see bands between 115-160kDa for this particular target. This weight varies from the theoretical molecular weight of 76kDa due to glycosylation of the protein.

  • Q: Would this antibody apply to tissue that fixed after the formalin? 

    A: Formalin fixation should be acceptable for use with this antibody. We incubate freshly dissected tissue in 10% formalin for 4-24 hours, although the incubation time is dependent on tissue type and size. We do not recommend fixation for longer than 24 hours since this can cause epitope masking.

  • Q: Can you give me more information about this antibody?

    A: Our mouse monoclonal antibody CD31/PECAM-1 Antibody (TLD-3A12) NB100-64796 was raised against activated Lewis rat derived microglial cells. Since we do not routinely epitope map our antibodies however, I am unable to tell you exactly where on the protein it binds.

  • Q: I potentially am interested in CD31/PECAM1 Antibody (TLD-3A12). I am woundering if this AB is suitable to use for immunohistochemistry with PFA-fixed section. Is it suitable?

    A: Yes, this antibody is suitable for IHC with PFA

  • Q: What is the concentration NB100-64796?

    A: The volume of the antibody provided is 250ul. The concentration of the antibody is 1.0mg/ml.

  • Q: What is the size of the target protein CD31/PECAM for this antibody?

    A: In western blot, we see bands between 115-160kDa for this particular target. This weight varies from the theoretical molecular weight of 76kDa due to glycosylation of the protein.

  • Q: Would this antibody apply to tissue that fixed after the formalin? 

    A: Formalin fixation should be acceptable for use with this antibody. We incubate freshly dissected tissue in 10% formalin for 4-24 hours, although the incubation time is dependent on tissue type and size. We do not recommend fixation for longer than 24 hours since this can cause epitope masking.

Showing  1 - 5 of 5 FAQs Showing All
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