CD40/TNFRSF5 Antibody (BLR056F)

Novus Biologicals | Catalog # NBP2-76366

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR056F
View all CD40/TNFRSF5 Primary Antibodies »

Product Specifications

Immunogen

Immunogen sequence between amino acids 227 and 277 (C-Term)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD40/TNFRSF5 Antibody (BLR056F)

Western Blot: CD40/TNFRSF5 Antibody (BLR056F) [NBP2-76366]

Western Blot: CD40/TNFRSF5 Antibody (BLR056F) [NBP2-76366]

Western Blot: CD40/TNFRSF5 Antibody (BLR056F) [NBP2-76366] - Detection of human CD40 by WB of Raji, HEK293T, U2OS, GaMG, Daudi, A-549, Ramos, HDLM-2, RKO, Jurkat, and MJ lysate. Antibody: Rabbit anti-CD40 recombinant monoclonal [BLR056F] (NBP2-76366). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-RPS3.
Immunocytochemistry/ Immunofluorescence: CD40/TNFRSF5 Antibody (BLR056F) [NBP2-76366]

Immunocytochemistry/ Immunofluorescence: CD40/TNFRSF5 Antibody (BLR056F) [NBP2-76366]

Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (BLR056F) [NBP2-76366] - Detection of human CD40 (red) in FFPE breast carcinoma by IHC-IF. Antibody: Rabbit anti-CD40 recombinant monoclonal [BLR056F] NBP2-76366. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).
Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (BLR056F) [NBP2-76366]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (BLR056F) [NBP2-76366]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (BLR056F) [NBP2-76366] - Detection of human CD40 in FFPE tonsil by IHC. Antibody: Rabbit anti-CD40 recombinant monoclonal [BLR056F] (NBP2-76366). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunocytochemistry/ Immunofluorescence: CD40/TNFRSF5 Antibody (BLR056F) [NBP2-76366]

Immunocytochemistry/ Immunofluorescence: CD40/TNFRSF5 Antibody (BLR056F) [NBP2-76366]

Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (BLR056F) [NBP2-76366] - Detection of human CD40 in FFPE Raji cells by ICC. Antibody: Rabbit anti-CD40 recombinant monoclonal [BLR056F] (NBP2-76366). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (BLR056F) [NBP2-76366]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (BLR056F) [NBP2-76366]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (BLR056F) [NBP2-76366] - Detection of human CD40 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-CD40 recombinant monoclonal [BLR056F] (NBP2-76366). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunoprecipitation: CD40/TNFRSF5 Antibody (BLR056F) [NBP2-76366]

Immunoprecipitation: CD40/TNFRSF5 Antibody (BLR056F) [NBP2-76366]

Immunoprecipitation: CD40/TNFRSF5 Antibody (BLR056F) [NBP2-76366] - Detection of human CD40 by WB of immunoprecipitates from Raji lysate. Antibodies: Rabbit anti-CD40 recombinant monoclonal [BLR056F] (NBP2-76366) and another rabbit anti-CD40 recombinant monoclonal.

Applications for CD40/TNFRSF5 Antibody (BLR056F)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100- 1:500

Immunohistochemistry

1:100 - 1:500

Immunoprecipitation

5 ul/1 mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: CD40/TNFRSF5

CD40 and its ligand CD154 are members of the tumor necrosis factor receptor (TNFR) and TNF families, respectively, that play key roles in signaling pathways mediating cell growth, survival and differentiation in B-lymphocytes (reviewed in Quezada et al 2004). The CD40 receptor is a 45-50 kDa glycoprotein and is expressed on the surface of B-lymphocytes, some activated T-cells, monocytes, follicular dendritic cells, basal epithelial cells, and in some epithelial and non-epithelial carcinomas. The functions of CD40 have been most extensively studied in B-cells. Ligation of B CD40 by CD154, expressed on activated T cells, stimulates B cell proliferation, differentiation, isotype switching, upregulation of surface molecules contributing to antigen presentation, development of the germinal center, and the humoral memory response. Several distinct structural motifs in the CD40 cytoplasmic domain regaulate various CD40 signaling pathways. A major CD40 signaling pathway activated from CD154 ligand binding is the canonical pathway to the transcription factor family NF-kB, a family of genes mediating immune and inflammatory responses. Although CD40 has been extensively studied as a plasma membrane-associated growth factor membrane receptor. it has also been identified in the cytoplasm and nucleus of normal and neoplastic B-lymphoid cells (Lin-Lee et al. 2006). Other growth factor receptors, including EGF, FGF, and TGF-B have also been identified in the nuclus. It is thought that plasma membrane receptor signaling may be followed by nuclear migration of signaling pathway components. The presence of CD40 in the nucleus of activated normal B lymphocytes and neoplastic B-lymphoid cells suggests that CD40 may play a more complex role in regulating essential growth and survival pathways in B-lymphocytes than previously thought.

Alternate Names

CD40, TNFRSF5

Gene Symbol

CD40

Additional CD40/TNFRSF5 Products

Product Documents for CD40/TNFRSF5 Antibody (BLR056F)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD40/TNFRSF5 Antibody (BLR056F)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD40/TNFRSF5 Antibody (BLR056F)

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I wanna know does CD40 antibody for IHC-P comes with positive control slide?

    A: Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.

  • Q: We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?

    A: Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

  • Q: I wanna know does CD40 antibody for IHC-P comes with positive control slide?

    A: Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.

  • Q: We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?

    A: Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

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