CD44 Antibody (CL13328) - BSA Free

Name: CD44 Antibody (CL13328) - BSA Free
Brand: Novus Biologicals
SKU: NBP3-18558
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-18558

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₃ Clone # CL13328

Format

BSA Free

View all CD44 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from P16070, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₃

Scientific Data Images for CD44 Antibody (CL13328) - BSA Free

Western Blot: CD44 Antibody (CL13328) [NBP3-18558]

Western Blot: CD44 Antibody (CL13328) [NBP3-18558] - Analysis in human cell line U-251 MG and human cell line MCF-7.

Immunohistochemistry-Paraffin: CD44 Antibody (CL13328) [NBP3-18558]

Immunohistochemistry-Paraffin: CD44 Antibody (CL13328) [NBP3-18558] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: CD44 Antibody (CL13328) [NBP3-18558]

Immunohistochemistry-Paraffin: CD44 Antibody (CL13328) [NBP3-18558] - Analysis in human cervix, uterine and skeletal muscle tissues using NBP3-18558 antibody. Corresponding CD44 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: CD44 Antibody (CL13328) [NBP3-18558]

Immunohistochemistry-Paraffin: CD44 Antibody (CL13328) [NBP3-18558] - Staining of human malignant melanoma shows moderate membranous positivity in tumor cells.

Immunohistochemistry-Paraffin: CD44 Antibody (CL13328) [NBP3-18558]

Immunohistochemistry-Paraffin: CD44 Antibody (CL13328) [NBP3-18558] - Staining of human ovarian cancer (low grade carcinoma) shows strong membranous positivity in tumor cells.

Immunohistochemistry-Paraffin: CD44 Antibody (CL13328) [NBP3-18558]

Immunohistochemistry-Paraffin: CD44 Antibody (CL13328) [NBP3-18558] - Staining of human breast cancer (metastasis to ovary) shows moderate membranous positivity in tumor cells.

Immunohistochemistry-Paraffin: CD44 Antibody (CL13328) [NBP3-18558]

Immunohistochemistry-Paraffin: CD44 Antibody (CL13328) [NBP3-18558] - Staining of human uterine cervix shows strong membranous positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: CD44 Antibody (CL13328) [NBP3-18558]

Immunohistochemistry-Paraffin: CD44 Antibody (CL13328) [NBP3-18558] - Staining of human lymph node shows strong membranous positivity in lymphoid cells.

Immunohistochemistry-Paraffin: CD44 Antibody (CL13328) [NBP3-18558]

Immunohistochemistry-Paraffin: CD44 Antibody (CL13328) [NBP3-18558] - Staining of human colon shows strong membranous positivity in lymphoid cells.

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Applications for CD44 Antibody (CL13328) - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml

Application Notes

For use with IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD44

CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.1-6

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Gene Symbol

CD44

Additional CD44 Products

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Product Specific Notices for CD44 Antibody (CL13328) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.