CD44 Antibody (OX-50) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80649
Key Product Details
Species Reactivity
Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OX-50
Format
Azide and BSA Free
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Product Specifications
Immunogen
Rat T cell blasts from a mixed lymphocyte reaction between purified CD4+ T cells and irradiated spleen cells.
Localization
Type I membrane protein.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CD44 Antibody (OX-50) - Azide and BSA Free
![Immunocytochemistry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]](https://resources.rndsystems.com/images/products/CD44-Antibody-OX-50-Azide-and-BSA-Free-Immunocytochemistry-NBP2-80649-img0001.jpg "Immunocytochemistry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]")
Immunocytochemistry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]
Immunocytochemistry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649] - Analysis of a FFPE tissue section of Rat small intestine using Mouse anti-Rat CD44 antibody (clone OX50) at 1:100 dilution. The staining was detected using HRP-conjugated anti-Mouse secondary antibody with DAB reagent followed by hematoxylin counterstaini![Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]](https://resources.rndsystems.com/images/products/CD44-Antibody-OX-50-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80649-img0002.jpg "Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]")
Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]
Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649] - Analysis using the FITC conjugate of NB600-1317. Staining of rat spleen cells with Mouse anti Rat CD44: FITC.![Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]](https://resources.rndsystems.com/images/products/CD44-Antibody-OX-50-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80649-img0003.jpg "Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]")
Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]
Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649] - Rat Splenocytes were stained with CD44 (OX-50) antibody NB600-1317(blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.Applications for CD44 Antibody (OX-50) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:50 - 1:100
Immunohistochemistry
1:50 - 1:100
Immunohistochemistry-Frozen
1:50 - 1:100. Use reported in scientific literature (PMID 16673425)
Immunohistochemistry-Paraffin
1:50 - 1:100. Use reported in scientific literature (PMID 16673425)
Western Blot
reported in scientific literature (PMID 8537452)
Application Notes
This antibody is CyTOF ready.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD44
Alternate Names
CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1
Gene Symbol
CD44
Additional CD44 Products
Product Documents for CD44 Antibody (OX-50) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CD44 Antibody (OX-50) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Hematopoietic Stem Cell Differentiation Pathways & Lineage-specific Markers
Innate Lymphoid Cell Differentiation Pathways
Mesenchymal Stem Cell Differentiation Pathways & Lineage-specific Markers
Neural Stem Cell Differentiation Pathways & Lineage-specific Markers
