CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-44877

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Human (Negative), Rat (Negative)

Applications

Western Blot, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Functional, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 1aG4/C5

Format

Azide and BSA Free
View all CD90/Thy1 Primary Antibodies »

Product Specifications

Immunogen

thymocytes from C3H/Di mice (alelic form: Thy-1.2 positive)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Marker

Mesenchymal Cell Marker

Specificity

The antibody 1aG4/C5 recognizes alloantigen Thy-1.2 of mouse Thy-1 (CD90); it does not cross-react with Thy-1.1 alloantigen. Thy-1 is a 18 kDa cell surface GPI linked glycoprotein (Immunoglobulin superfamily); in the mouse it is expressed on all thymocytes and peripheral T lymphocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free

Flow (Cell Surface): CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free [NBP1-44877]

Flow (Cell Surface): CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free [NBP1-44877]

Flow (Cell Surface): CD90/Thy1 Antibody (1aG4/C5) - Azide Free [NBP1-44877] - Surface staining of murine splenocytes using anti-CD90.2 (1aG4/C5) PE.
Flow (Cell Surface): CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free [NBP1-44877]

Flow (Cell Surface): CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free [NBP1-44877]

Flow (Cell Surface): CD90/Thy1 Antibody (1aG4/C5) - Azide Free [NBP1-44877] - Surface staining of murine splenocytes using anti-CD90.2 (1aG4/C5) purified, DAR-PE.

Applications for CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:1000
Application Notes
Functional Application: The bound of antibody 1aG4/C5 to the transfected mast cells induces the activation processes which are similar, but not identical, to the antigen activation of IgE stimulated cells. Other studies approve that the antibody 1aG4/C5 precipitates complexes with Lyn protein tyrosine kinase. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD90/Thy1

This antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Alternate Names

CD90, Thy1

Gene Symbol

THY1

Additional CD90/Thy1 Products

Product Documents for CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for CD90/Thy1 Antibody (1aG4/C5) - Azide and BSA Free

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  • Q: We are interested in CD90 antibodies that could react with dog, please let me know which products in your catalog you would recommend.

    A: I would recommend one of theses CD90 antibodies, as these have all been validated for use with dog/canine samples.

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