CDX2 Antibody (9E0K5)

Novus Biologicals | Catalog # NBP3-57371

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9E0K5
View all CDX2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide. This information is considered to be commercially sensitive. (Swissprot# Q99626)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

Normal Tissues Tested
Fallopian tube, Pancreas, Lung, Kidney, Placenta, Stomach, Liver, Thyroid, Appendix, Testis, Prostate, Tonsil, Cervix, esophagus, Breast, Colon, Brain

Cancer Tissues Tested
Lung adenocarcinoma, Lung squamous carcinoma, Hepatocellular Carcinoma, Hepatocholangio carcinoma, Thyroid Cancer, Prostate cancer, cervical squamous cell carcinoma, Breast Cancer, Colon Cancer

Positive Controls
Appendix/colon (columnar epithelial cells), Colon Cancer, Pancreas (all ductal and intercalated duct epithelial cells should demonstrate at least weak to moderate, distinct nuclear staining reaction)

Negative Controls
Appendix/colon (leukomonocyte, Fibroblasts), tonsil, liver, Placenta, Breast

Scientific Data Images for CDX2 Antibody (9E0K5)

CDX2 Antibody (9E0K5) Immunohistochemistry: CDX2 Antibody (9E0K5) [NBP3-57371]

Immunohistochemistry: CDX2 Antibody (9E0K5) [NBP3-57371]

Confocal imaging of Human colon using NBP3-57371 (dilution 1:100)(Red). DAPI was used for nuclear staining (blue). Objective: 40x.
CDX2 Antibody (9E0K5) Immunoprecipitation: CDX2 Antibody (9E0K5) [NBP3-57371]

Immunoprecipitation: CDX2 Antibody (9E0K5) [NBP3-57371]

Immunoprecipitation analysis of 600 ug extracts of 293T cells using 3 ug NBP3-57371. Western blot was performed from the immunoprecipitate using NBP3-57371 at a dilution of 1:500.
CDX2 Antibody (9E0K5) Knockout Validated: CDX2 Antibody (9E0K5) [NBP3-57371]

Knockout Validated: CDX2 Antibody (9E0K5) [NBP3-57371]

Western blot analysis of lysates from wild type(WT) and CDX2 knockdown (KD) 293T cells, using NBP3-57371 at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.
CDX2 Antibody (9E0K5) Western Blot: CDX2 Antibody (9E0K5) [NBP3-57371]

Western Blot: CDX2 Antibody (9E0K5) [NBP3-57371]

Western blot analysis of various lysates using NBP3-57371 at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 3min.
CDX2 Antibody (9E0K5) Immunohistochemistry-Paraffin: CDX2 Antibody (9E0K5) [NBP3-57371]

Immunohistochemistry-Paraffin: CDX2 Antibody (9E0K5) [NBP3-57371]

Confocal imaging of paraffin-embedded Mouse colon tissue using NBP3-57371 (dilution 1:500) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
CDX2 Antibody (9E0K5) Immunohistochemistry-Paraffin: CDX2 Antibody (9E0K5) [NBP3-57371]

Immunohistochemistry-Paraffin: CDX2 Antibody (9E0K5) [NBP3-57371]

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using NBP3-57371 at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
CDX2 Antibody (9E0K5) Immunohistochemistry-Paraffin: CDX2 Antibody (9E0K5) [NBP3-57371]

Immunohistochemistry-Paraffin: CDX2 Antibody (9E0K5) [NBP3-57371]

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using NBP3-57371 at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
CDX2 Antibody (9E0K5) Immunohistochemistry-Paraffin: CDX2 Antibody (9E0K5) [NBP3-57371]

Immunohistochemistry-Paraffin: CDX2 Antibody (9E0K5) [NBP3-57371]

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using NBP3-57371 at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
CDX2 Antibody (9E0K5) Immunohistochemistry-Paraffin: CDX2 Antibody (9E0K5) [NBP3-57371]

Immunohistochemistry-Paraffin: CDX2 Antibody (9E0K5) [NBP3-57371]

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using NBP3-57371 at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Applications for CDX2 Antibody (9E0K5)

Application
Recommended Usage

ELISA

1 ug/mL

Immunohistochemistry-Paraffin

1:3000 - 1:12000

Immunoprecipitation

0.5ug-4ug antibody for 200ug-600ug extracts of whole cells

Western Blot

1:500 - 1:2000
Application Notes
For immunofluorescence on paraffin-embedded tissues, the recommended concentration is 1:100 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, 0.05% BSA, (pH 7.3)

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDX2

The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear proteins that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. The proteins LMX1 (MIM 600298) and CDX3 are homeodomain proteins that bind an A/T-rich sequence in the insulin promoter and stimulate its transcription (German et al., 1994 [PubMed 7698771]).[supplied by OMIM]

Long Name

Caudal-type Homeobox Transcription Factor 2

Alternate Names

CDX3

Gene Symbol

CDX2

Additional CDX2 Products

Product Documents for CDX2 Antibody (9E0K5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CDX2 Antibody (9E0K5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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