CDX2 Antibody (CL12967) - BSA Free

Novus Biologicals | Catalog # NBP3-18571

Novus Biologicals

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL12967

Format

BSA Free
View all CDX2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from Q99626, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse CDX2 Antibody (CL12967) - BSA Free (NBP3-18571) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CDX2 Antibody (CL12967) - BSA Free

Immunocytochemistry/ Immunofluorescence: CDX2 Antibody (CL12967) [NBP3-18571]

Immunocytochemistry/ Immunofluorescence: CDX2 Antibody (CL12967) [NBP3-18571]

Immunocytochemistry/Immunofluorescence: CDX2 Antibody (CL12967) [NBP3-18571] - Analysis in human cell line CACO-2 and human cell line PC-3.
Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Immunocytochemistry/ Immunofluorescence: CDX2 Antibody (CL12967) [NBP3-18571]

Immunocytochemistry/ Immunofluorescence: CDX2 Antibody (CL12967) [NBP3-18571]

Immunocytochemistry/Immunofluorescence: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of CACO-2 cells using the Anti-CDX2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probes are visualized in red (where available).
Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Analysis in human colon and tonsil tissues using NBP3-18571 antibody. Corresponding CDX2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human colorectal cancer (metastasis to ovary) shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human colon shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human rectum shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12967) [NBP3-18571] - Staining of human liver shows no positivity in hepatocytes as expected.

Applications for CDX2 Antibody (CL12967) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. For use with ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDX2

The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear proteins that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. The proteins LMX1 (MIM 600298) and CDX3 are homeodomain proteins that bind an A/T-rich sequence in the insulin promoter and stimulate its transcription (German et al., 1994 [PubMed 7698771]).[supplied by OMIM]

Long Name

Caudal-type Homeobox Transcription Factor 2

Alternate Names

CDX3

Gene Symbol

CDX2

Additional CDX2 Products

Product Documents for CDX2 Antibody (CL12967) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CDX2 Antibody (CL12967) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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