CDX2 Antibody (CL12974) - BSA Free

Novus Biologicals | Catalog # NBP3-18552

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL12974

Format

BSA Free
View all CDX2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from Q99626, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CDX2 Antibody (CL12974) - BSA Free

Western Blot: CDX2 Antibody (CL12974) [NBP3-18552]

Western Blot: CDX2 Antibody (CL12974) [NBP3-18552]

Western Blot: CDX2 Antibody (CL12974) [NBP3-18552] - Analysis in human cell line CACO-2 and human cell line PC-3.
Immunocytochemistry/ Immunofluorescence: CDX2 Antibody (CL12974) [NBP3-18552]

Immunocytochemistry/ Immunofluorescence: CDX2 Antibody (CL12974) [NBP3-18552]

Immunocytochemistry/Immunofluorescence: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of CACO-2 cells using the Anti-CDX2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule is visualized in red (where available).
Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.
Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552] - Analysis in human rectum and lymph node tissues using NBP3-18552 antibody. Corresponding CDX2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of human colorectal cancer (metastasis to ovary) shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of human rectum shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552]

Immunohistochemistry-Paraffin: CDX2 Antibody (CL12974) [NBP3-18552] - Staining of human colon shows moderate nuclear positivity in glandular cells.

Applications for CDX2 Antibody (CL12974) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDX2

The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear proteins that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. The proteins LMX1 (MIM 600298) and CDX3 are homeodomain proteins that bind an A/T-rich sequence in the insulin promoter and stimulate its transcription (German et al., 1994 [PubMed 7698771]).[supplied by OMIM]

Long Name

Caudal-type Homeobox Transcription Factor 2

Alternate Names

CDX3

Gene Symbol

CDX2

Additional CDX2 Products

Product Documents for CDX2 Antibody (CL12974) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CDX2 Antibody (CL12974) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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