CEACAM1/CD66a Antibody (B3-17) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-36277

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # B3-17

Format

Azide and BSA Free
View all CEACAM-1/CD66a Primary Antibodies »

Product Specifications

Immunogen

recombinant human soluble CEACAM-1-Fc (produced in HEK293 cells)

Specificity

Anti-human CEACAM-1 (B3) interacts solely with human CEACAM-1 and shows no cross-reactivity with other CEACAMs expressed in human or other species, Clone B3 binds to the A1-B-Domain.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for CEACAM1/CD66a Antibody (B3-17) - Azide and BSA Free

Application
Recommended Usage

ELISA

10ug/ml

Flow Cytometry

10ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

10ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

5 ug/ml
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CEACAM-1/CD66a

CEACAM1 encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of only two has been determined. [provided by RefSeq]

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 1

Alternate Names

BGP1, Biliary Glycoprotein 1, CD66a, Cea-1, CEACAM1, Hv-1, Hv-2, MHVR

Gene Symbol

CEACAM1

Additional CEACAM-1/CD66a Products

Product Documents for CEACAM1/CD66a Antibody (B3-17) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CEACAM1/CD66a Antibody (B3-17) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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