CFTR Antibody (CF3)

Novus Biologicals | Catalog # NB300-511

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken, Golden Syrian Hamster

Cited:

Human, Mouse, Rat, Avian - Chicken, Golden Syrian Hamster

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # CF3
View all CFTR Primary Antibodies »

Product Specifications

Immunogen

Synthetic Peptide: G(103) R I I A S Y D P D N K E E R(117)

Reactivity Notes

Rat reactivity reported in scientific literature (see product references). Chicken reactivity reported in scientific literature (PMID: 12505864). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Use in Golden Syrian Hamster reported in scientific literature (PMID:22534621).

Localization

Membrane; multi-pass membrane protein.

Specificity

CFTR (CF3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Theoretical MW

168 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CFTR Antibody (CF3)

Immunocytochemistry/ Immunofluorescence: CFTR Antibody (CF3) [NB300-511]

Immunocytochemistry/ Immunofluorescence: CFTR Antibody (CF3) [NB300-511]

Immunocytochemistry/Immunofluorescence: CFTR Antibody (CF3) [NB300-511] - CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight 488 conjugated antibody.
Immunohistochemistry-Paraffin: CFTR Antibody (CF3) [NB300-511]

Immunohistochemistry-Paraffin: CFTR Antibody (CF3) [NB300-511]

Immunohistochemistry-Paraffin: CFTR Antibody (CF3) [NB300-511] - Normal biopsies of deparaffinized Human tonsil tissue.
Flow Cytometry: CFTR Antibody (CF3) [NB300-511]

Flow Cytometry: CFTR Antibody (CF3) [NB300-511]

Flow Cytometry: CFTR Antibody (CF3) [NB300-511] - Human airway epithelial cells stained with CFTR (CF3) coupled with goat anti-mouse FITC conjugated secondary antibody. Flow cytometry image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: CFTR Antibody (CF3) [NB300-511]

Immunocytochemistry/ Immunofluorescence: CFTR Antibody (CF3) [NB300-511]

Immunocytochemistry/Immunofluorescence: CFTR Antibody (CF3) [NB300-511] - Staining of human pancreas cryosection (200x). Alpha cells are strongly labeled. ICC/IF image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: CFTR Antibody (CF3) [NB300-511]

Immunocytochemistry/ Immunofluorescence: CFTR Antibody (CF3) [NB300-511]

Immunocytochemistry/Immunofluorescence: CFTR Antibody (CF3) [NB300-511] - CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight 488 conjugated antibody.
Immunocytochemistry/ Immunofluorescence: CFTR Antibody (CF3) [NB300-511]

Immunocytochemistry/ Immunofluorescence: CFTR Antibody (CF3) [NB300-511]

Immunocytochemistry/Immunofluorescence: CFTR Antibody (CF3) [NB300-511] - CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight 488 conjugated antibody.
Immunohistochemistry-Paraffin: CFTR Antibody (CF3) [NB300-511]

Immunohistochemistry-Paraffin: CFTR Antibody (CF3) [NB300-511]

Immunohistochemistry-Paraffin: CFTR Antibody (CF3) [NB300-511] - Cancer biopsies of deparaffinized Human colon carcinoma tissue.
Immunohistochemistry-Paraffin: CFTR Antibody (CF3) [NB300-511]

Immunohistochemistry-Paraffin: CFTR Antibody (CF3) [NB300-511]

Immunohistochemistry-Paraffin: CFTR Antibody (CF3) [NB300-511] - Normal biopsies of deparaffinized Human pancreas tissue.

Applications for CFTR Antibody (CF3)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10 - 1:500

Western Blot

1:500
Application Notes
Flow usage reported in literature and validated by a verified customer review. ICC usage reported in literature. IHC usage was reported in scientific literature. Blocking Assay was reported in the scientific literature (PMID: 9590693). WB: Detects an approx. 170 kDa protein. Also detects one or more immunologically related proteins in murine cell line Heb7a that does not contain CFTR mRNA. IF: Staining of CFTR in mouse epithelial cells results in cell surface staining, consistent with localization at the plasma membrane. Inhibit the epithelial uptake of S. typhi in some mouse cell lines.

Reviewed Applications

Read 2 reviews rated 4.5 using NB300-511 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CFTR

Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508) which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is. One possible explanation is that Salmonella typhi has been shown to use CFTR to enter intestinal epithelial cells and that delta F508 heterozygote and homozygote mice showed 86% and 100% reductions in S.typhi intestinal submucosal uptake.

Long Name

Cystic Fibrosis Transmembrane Conductance Regulator

Alternate Names

ABC35, ABCC7CF, ATP-binding cassette sub-family C member 7, ATP-binding cassette transporter sub-family C member 7, cAMP-dependent chloride channel, CFTR/MRP, Channel conductance-controlling ATPase, cystic fibrosis transmembrane conductance regulator, cystic fibrosis transmembrane conductance regulator (ATP-binding cassettesub-family C, member 7), cystic fibrosis transmembrane conductance regulator, ATP-binding cassette(sub-family C, member 7), dJ760C5.1, EC 3.6.3, MRP7EC 3.6.3.49, TNR-CFTR

Entrez Gene IDs

1080 (Human); 12638 (Mouse)

Gene Symbol

CFTR

UniProt

P13569

Additional CFTR Products

Product Documents for CFTR Antibody (CF3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CFTR Antibody (CF3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CFTR Antibody (CF3)

Customer Reviews for CFTR Antibody (CF3) (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • CFTR Antibody (CF3)
    Name: James Xiao
    Application: Flow Cytometry
    Sample Tested: human airway epithelial cells
    Species: Human
    Verified Customer | Posted 09/30/2020
    Human airway epithelial cells stained with CFTR (CF3) coupled with goat anti-mouse FITC conjugated 2nd ab.
    CFTR Antibody (CF3) NB300-511
  • Name: Anonymous
    Application: Immunofluorescence
    Verified Customer | Posted 05/09/2014
    NB300-511 on human pancreas cryosection (200x). Alpha cells are strongly labeled.
    CFTR Antibody (CF3) NB300-511

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Protocols

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