Choline Acetyltransferase/ChAT Antibody (CL3173) - BSA Free

Novus Biologicals | Catalog # NBP2-46620

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL3173

Format

BSA Free
View all Choline Acetyltransferase/ChAT Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P28329, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Choline Acetyltransferase/ChAT Antibody (CL3173) - BSA Free

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining in human placenta and liver tissues. Corresponding CHAT RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining of SK-MEL-30 cells using the Anti-CHAT monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]
Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining of mouse brain shows strong positivity in acetylcholine neurons in the basal forebrain.
Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining of mouse basal forebrain shows strong positivity in acetylcholine neurons in the caudate putamen.
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining of human cerebral cortex shows moderate positivity in cholinergic neural fibers.
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining of human placenta shows strong cytoplasmic positivity in a subset of cells in chorionic villi.
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunohistochemistry: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining of rat brain shows strong positivity in acetylcholine neurons in the basal forebrain.

Applications for Choline Acetyltransferase/ChAT Antibody (CL3173) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Choline Acetyltransferase/ChAT

Note: This is the same product as NB300-178. FUNCTION: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. SIMILARITY: Belongs to the carnitine/choline acetyltransferase family.

Alternate Names

CHAT, Choline acetylase

Entrez Gene IDs

1103 (Human)

Gene Symbol

CHAT

UniProt

P28329 (Human)

Additional Choline Acetyltransferase/ChAT Products

Product Documents for Choline Acetyltransferase/ChAT Antibody (CL3173) - BSA Free

Certificate of Analysis

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Product Specific Notices for Choline Acetyltransferase/ChAT Antibody (CL3173) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Choline Acetyltransferase/ChAT Antibody (CL3173) - BSA Free

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Protocols

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FAQs

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