Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CIDEA Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against an 18 amino acid peptide near the carboxy terminus of mouse CIDE-A. The immunogen is located within the last 50 amino acids of CIDE-A. Amino Acid Squence: GDTEEQPSPKPSTKG

Specificity

It has no cross activity to CIDE-B.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit CIDEA Antibody - BSA Free (NBP1-76950) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-CIDEA Antibody: Cited in 11 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CIDEA Antibody - BSA Free

Western Blot: CIDEA AntibodyBSA Free [NBP1-76950]

Western Blot: CIDEA AntibodyBSA Free [NBP1-76950]

Western Blot: CIDEA Antibody [NBP1-76950] - Mouse heart tissue lysate with CIDE-A antibody at 1:500 dilution.
CIDEA Antibody - BSA Free

Immunohistochemistry: CIDEA Antibody - BSA Free [NBP1-76950] -

Immunohistochemistry: CIDEA Antibody - BSA Free [NBP1-76950] - Immunohistochemistry of CIDEA in mouse heart tissue with CIDEA antibody at 5 ug/mL.
CIDEA Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CIDEA Antibody - BSA Free [NBP1-76950] -

Immunocytochemistry/ Immunofluorescence: CIDEA Antibody - BSA Free [NBP1-76950] - Immunofluorescence of CIDEA in Mouse Heart cells with CIDEA antibody at 20 ug/mL.
CIDEA Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CIDEA Antibody - BSA Free [NBP1-76950] -

Immunocytochemistry/ Immunofluorescence: CIDEA Antibody - BSA Free [NBP1-76950] - Immunofluorescence of CIDEA in human brain tissue with CIDE A antibody at 20 ug/mL.

Green: CIDEA Antibody
Blue: DAPI staining
CIDEA Antibody - BSA Free

Immunohistochemistry: CIDEA Antibody - BSA Free [NBP1-76950] -

Immunohistochemistry: CIDEA Antibody - BSA Free [NBP1-76950] - Immunohistochemistry of CIDEA in human brain tissue with CIDE A antibody at 2.5 ug/mL.
CIDEA Antibody - BSA Free

Western Blot: CIDEA Antibody - BSA Free [NBP1-76950] -

Hepatic expression of FSP27 beta strongly increased with the severity of NAFLD whereas CIDEA expression decreased. Wild-type mice fed a control diet (Ctrl D) (n = 4) or MCDD (n = 6) for 2 and 7 weeks. (A, B, C) Hepatic expression of CIDEA, CIDEB, FSP27 alpha and FSP27 beta was evaluated in Wt, Ctrl D and MCDD mice at the mRNA level (4–6 mice/group). The gene expression was normalized to the mRNA levels of B2M or 36B4. Results are expressed relative to the expression level in controls (means +/- SEM) and statistically analyzed using the Mann–Whitney test. (D) Hepatic expression of CIDEA, FSP27 and HSP90 was evaluated in Wt, Ctrl D and MCDD mice at the protein level (2–5 mice/group). *, versus Ctrl D, p < 0.05 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31097771), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CIDEA Antibody - BSA Free

Western Blot: CIDEA Antibody - BSA Free [NBP1-76950] -

Hepatic expression of CIDEA, FSP27 alpha and FSP27 beta increased with hepatic steatosis induced by HFD challenge. Wild-type mice fed a control diet (Ctrl D) (n = 7) or HFD (n = 7) for 33 weeks. (A, C) Hepatic expression of CIDEA, CIDEB, FSP27 alpha and FSB27 beta was evaluated in Wt, Ctrl D and HFD mice at the mRNA level (7 mice/group). The gene expression was normalized to the mRNA levels of B2M or 36B4. Results are expressed relative to the expression level in controls (means +/- SEM) and statistically analyzed using the Mann–Whitney test. (B) Hepatic expression of CIDEA, FSP27 and HSP90 was evaluated in Wt, Ctrl D and HFD mice at the protein level (2–4 mice/group). *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31097771), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CIDEA Antibody - BSA Free

Western Blot: CIDEA Antibody - BSA Free [NBP1-76950] -

Overexpression of CIDEA promoted apoptosis in ESCC cells. (A) Cisplatin treated cells were stained for Annexin V/PI and analyzed FACS. The percentage of Annexin V+ cells is shown. (B) Mitochondrial potential was measured by flow cytometry using the JC-1 molecular probe in ESCC cells with or without cisplatin treatment. Enhanced green fluorescence ratio of cells indicated a decrease in mitochondrial potential. (C) Cell viability of CIDEA overexpressing cells and control cells treated with different concentrations of cisplatin. (D) Caspase-9, PARP, and p-JNK were detected in CIDEA overexpressing cells and control cells with or without cisplatin treatment. (E) Quantification of p-JNK, Bad, cleaved-Caspase9, and cleaved-PARP in KYSE30 and KYSE150 cells treated with cisplatin. Statistical data are represented as mean +/- SD. *P < 0.05; **P < 0.01; ***P < 0.001; n.s., P > 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33614508), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CIDEA Antibody - BSA Free

Western Blot: CIDEA Antibody - BSA Free [NBP1-76950] -

Losartan reduced LDs accumulation and lipogenesis in liver. (A) Representative HE, Oil Red O, HIF1 alpha, PLIN1, PLIN2, and CIDEA staining of liver. Red arrow highlights the positive staining. Scale bar: 100 μm. Tissue staining, as quantified by either stain intensity, is represented on the left-hand vertical axis in each graph. (B) Quantification of HIF1 alpha, PLIN1, PLIN2, and CIDEA protein levels in homogenates of liver. Below graphs indicate quantification relative to Histone (for HIF1 alpha ) and beta -actin (for PLIN1, PLIN2, and CIDEA). (C) Quantification of Srebp-1c, Fas and Scd1, (D) Cd36, Fatp, Cpt1, and Cpt2 by qRT-PCR. For each animal group, n = 5. All values represent the mean +/- SEM. Data were analyzed by Student’s t test. * p ≤ 0.05, normal vs. ob/ob; # p ≤ 0.05, ob/ob vs. ob/ob+ Losartan. HIF1: hypoxia-inducible factor 1; LDs: lipid droplets; HE: hematoxylin and eosin stain; PLIN1, perilipin 1; CIDEA, cell death inducing DFFA like effector A; SREBP: sterol response element-binding protein; Fas: fatty acid synthase; Scd: stearoyl-CoA desaturase; Cd36: cluster of differentiation 36; Fatp: fatty acid transport protein; Cpt1: carnitine palmitoyltransferase 1; qRT-PCR: quantitative real time polymerase chain reaction. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34889901), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CIDEA Antibody - BSA Free

Western Blot: CIDEA Antibody - BSA Free [NBP1-76950] -

Losartan attenuated HIF-1 alpha expression and LDs formation in EWAT. (A) Representative HIF-1 alpha staining of EWAT from losartan-treated ob/ob mice and normal mice. Red arrow highlights the positive staining. Scale bar: 100 μm. Quantification of (B) HIF-1 alpha protein level by Western blot. Below graphs indicate quantification relative to Histone. (C) Representative HE, PLIN1, PLIN2, CIDEA and CIDEC staining of EWAT from losartan-treated ob/ob mice and normal mice. Scale bar: 100 μm. Quantification of (D) PLIN1, PLIN2, CIDEA, and CIDEC protein levels by Western blot of EWAT after losartan treatment. Below graphs indicate quantification relative to beta -actin. For each animal group, n = 5. All values represent the mean +/- SEM. Data were analyzed by Student’s t test. * p ≤ 0.05; normal vs. ob/ob. # p ≤ 0.05; ob/ob vs. ob/ob + Losartan. HIF-1 alpha, hypoxia-inducible factor-1 alpha ; LDs, lipid droplets; EWAT, epididymis white adipose tissue; HE, hematoxylin and eosin; PLIN, perilipin; CIDE, cell-death-inducing DNA-fragmentation-factor (DFF45)-like effector. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34360607), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CIDEA Antibody - BSA Free

Western Blot: CIDEA Antibody - BSA Free [NBP1-76950] -

Losartan decreased HIF-1 alpha and LD-associated proteins. (A) Representative HE, Oil Red O, HIF-1 alpha, PLIN1, PLIN2, CIDEA and CIDEC staining of liver from normal mice and losartan-treated ob/ob mice. Green pseudo-color represents visualization of lipofuscin’s autofluorescence at 450–490 nm. Red arrow highlights the positive staining. Scale bar: 100 μm. Quantification of (B) HIF-1 alpha, PLIN1, PLIN2, CIDEA, and CIDEC protein levels by Western blot of liver after losartan treatment. Below graphs indicate quantification relative to Histone or beta -actin. (C) Quantification of ATGL, HSL, LPL, and ACO by qRT-PCR. qRT-PCR indicates quantification relative to GAPDH. For each animal group, n = 5. All values represent the mean +/- SEM. Data were analyzed by Student’s t test. * p ≤ 0.05; normal vs. ob/ob. # p ≤ 0.05; ob/ob vs. ob/ob + Losartan. HIF-1 alpha, hypoxia-inducible factor-1 alpha ; LDs, lipid droplets; HE, hematoxylin and eosin; PLIN, perilipin; CIDE, cell-death-inducing DNA-fragmentation-factor (DFF45)-like effector; ATGL, adipose triglyceride lipase; HSL, hormone-sensitive lipase; LPL, lipoprotein lipase; ACO, acyl-CoA oxidase. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34360607), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CIDEA Antibody - BSA Free

Western Blot: CIDEA Antibody - BSA Free [NBP1-76950] -

Losartan attenuated HIF1 alpha expression and LDs formation in EWAT. (A) Representative HE and HIF1 alpha staining. Red arrow highlights the positive staining. Scale bar: 100 μm. Tissue staining, as quantified by either stain intensity, is represented on the left-hand vertical axis in each graph. (B) Representative PLIN1, PLIN2, CIDEA, and CIDEC staining of EWAT. Green pseudocolor represents visualization of lipofuscin’s autofluorescence at 450–490 nm. Red arrow highlights the positive staining. Scale bar: 100 μm. Tissue staining, as quantified by either stain intensity, is represented on the left-hand vertical axis in each graph. Quantification of (C) HIF1 alpha protein levels by Western blot. Below graph indicate quantification relative to Histone. Quantification of (D) PLIN1, PLIN2, CIDEA, and CIDEC protein levels by Western blot of EWAT. Below graphs indicate quantification relative to beta -actin. For each animal group, n = 5. All values represent the mean +/- SEM. Data were analyzed by Student’s t test. * p ≤ 0.05, normal vs. ob/ob; # p ≤ 0.05, ob/ob vs. ob/ob+ Losartan. HIF1 alpha : hypoxia-inducible factor 1 alpha ; LDs: lipid droplets; EWAT: epididymal white adipose tissue; HE: hematoxylin and eosin stain; PLIN1: perilipin 1; CIDEA: cell death inducing DFFA like effector A. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34889901), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CIDEA Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/mL

Western Blot

0.5 ug/mL

Reviewed Applications

Read 1 review rated 3 using NBP1-76950 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CIDEA

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-A is expressed in many tissues.

Long Name

Cell Death-inducing DFFA-like Effector A

Alternate Names

cell death activator CIDE-A, cell death-inducing DFFA-like effector aCIDE-A

Gene Symbol

CIDEA

UniProt

AAC34985 (Mouse)

Additional CIDEA Products

Product Documents for CIDEA Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CIDEA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CIDEA Antibody - BSA Free

Customer Reviews for CIDEA Antibody - BSA Free (1)

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  • CIDEA Antibody
    Name: Johana Lambert
    Application: Western Blot
    Sample Tested: Mesenchymal stem cells differentiated into adipocytes
    Species: Mouse
    Verified Customer | Posted 11/16/2018
    Loaded 20 ug protein. Blocked 1 hour at room temp in 5% BSA (TBST). Incubated primary overnight at 4 degrees C, 1:1000 in 5% BSA (TBST)
    Dark bands at ~15 and ~37. Difficult to determine which, if either is CIDEA.
    CIDEA Antibody - BSA Free NBP1-76950

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