CLEC4E Antibody (16E3) - BSA Free

Novus Biologicals | Catalog # NBP1-49311

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Flow Cytometry, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 16E3

Format

BSA Free
View all CLEC4E Primary Antibodies »

Product Specifications

Immunogen

Recombinant human CLEC4E (41-219aa) purified from E. coli (NP_055173).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 31725411).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CLEC4E Antibody (16E3) - BSA Free

Western Blot: CLEC4E Antibody (16E3)BSA Free [NBP1-49311]

Western Blot: CLEC4E Antibody (16E3)BSA Free [NBP1-49311]

CLEC4E-Antibody-16E3-Western-Blot-NBP1-49311-img0022.jpg
Immunocytochemistry/ Immunofluorescence: CLEC4E Antibody (16E3) - BSA Free [NBP1-49311]

Immunocytochemistry/ Immunofluorescence: CLEC4E Antibody (16E3) - BSA Free [NBP1-49311]

Immunocytochemistry/Immunofluorescence: CLEC4E Antibody (16E3) [NBP1-49311] - HeLa cells stained with monoclonal anti-human MINCLE antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).
Immunohistochemistry-Paraffin: CLEC4E Antibody (16E3) - BSA Free [NBP1-49311]

Immunohistochemistry-Paraffin: CLEC4E Antibody (16E3) - BSA Free [NBP1-49311]

Immunohistochemistry-Paraffin: CLEC4E Antibody (16E3) [NBP1-49311] - Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human MINCLE antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Flow Cytometry: CLEC4E Antibody (16E3) - BSA Free [NBP1-49311]

Flow Cytometry: CLEC4E Antibody (16E3) - BSA Free [NBP1-49311]

Flow Cytometry: CLEC4E Antibody (16E3) [NBP1-49311] - An intracellular stain was performed on U87MG cells with CLEC4E Antibody (16E3) NBP1-49311AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647
Western Blot: CLEC4E Antibody (16E3)BSA Free [NBP1-49311]

Western Blot: CLEC4E Antibody (16E3)BSA Free [NBP1-49311]

Western Blot: CLEC4E Antibody (16E3) [NBP1-49311] - Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CLEC4E (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot: CLEC4E Antibody (16E3)BSA Free [NBP1-49311]

Western Blot: CLEC4E Antibody (16E3)BSA Free [NBP1-49311]

Western Blot: CLEC4E Antibody (16E3) [NBP1-49311] - The Cell lysates (40ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-human MINCLE antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate Lane 2. : CLEC4E transfected 293T cell lysate
Western Blot: CLEC4E Antibody (16E3)BSA Free [NBP1-49311]

Western Blot: CLEC4E Antibody (16E3)BSA Free [NBP1-49311]

CLEC4E-Antibody-16E3-Western-Blot-NBP1-49311-img0021.jpg
Flow Cytometry: CLEC4E Antibody (16E3) - BSA Free [NBP1-49311]

Flow Cytometry: CLEC4E Antibody (16E3) - BSA Free [NBP1-49311]

Flow Cytometry: CLEC4E Antibody (16E3) [NBP1-49311] - Flow cytometry analysis of CLEC4E in LNCap cell line,staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.Isotype control antibody was mouse IgG (black line).
Flow Cytometry: CLEC4E Antibody (16E3) - BSA Free [NBP1-49311]

Flow Cytometry: CLEC4E Antibody (16E3) - BSA Free [NBP1-49311]

Flow Cytometry: CLEC4E Antibody (16E3) [NBP1-49311] - An intracellular stain was performed on U-937 cells with CLEC4E Antibody (16E3) NBP1-49311AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: CLEC4E Antibody (16E3) - BSA Free [NBP1-49311]

Flow Cytometry: CLEC4E Antibody (16E3) - BSA Free [NBP1-49311]

Flow Cytometry: CLEC4E Antibody (16E3) [NBP1-49311] - An intracellular stain was performed on U87MG cells with CLEC4E Antibody (16E3) NBP1-49311AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Applications for CLEC4E Antibody (16E3) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:250-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50

Western Blot

1:250-1:500

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CLEC4E

MINCLE (macrophage inducible C-type lectin, also called CLEC4E or CLECSF9), which is a diverse family of protein which was originally defined by their ability to recognize a wide range of ligand of carbohydrate structure. MINCLE expressed in macrophages subjected to several types of stress. It plays an essential role in response to trehalose-6,6'-dimycolate (TDM) and activated by a synthetic analogue, trehalose dibehenate (TDB). Recently it was reported that MINCLE is associated with an immunoreceptor tyrosine-based activation motif-containing Fc receptor ? chain (FcR?) and functions as an activating receptor for damaged self- and non-self-pathogenic fungi.

Long Name

C-type Lectin Domain Family 4, Member E

Alternate Names

CLECSF9, MINCLE

Entrez Gene IDs

26253 (Human)

Gene Symbol

CLEC4E

UniProt

Q9ULY5

Additional CLEC4E Products

Product Documents for CLEC4E Antibody (16E3) - BSA Free

Certificate of Analysis

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Product Specific Notices for CLEC4E Antibody (16E3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

C-type Lectin Family

Citations for CLEC4E Antibody (16E3) - BSA Free

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Protocols

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