CLEC4E Antibody (16E3) [Alexa Fluor® 488]

Novus Biologicals | Catalog # NBP1-49311AF488

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 16E3
View all CLEC4E Primary Antibodies »

Product Specifications

Immunogen

Recombinant human CLEC4E (41-219aa) purified from E. coli (NP_055173).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 31725411).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for CLEC4E Antibody (16E3) [Alexa Fluor® 488]

Flow Cytometry: CLEC4E Antibody (16E3) [Alexa Fluor® 488] [NBP1-49311AF488] - Activated human blood mononuclear cells (PBMCs). Image from verified customer review.
Flow Cytometry: CLEC4E Antibody (16E3) [Alexa Fluor® 488] [NBP1-49311AF488] - An intracellular stain was performed on U-937 cells with CLEC4E Antibody (16E3) NBP1-49311AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: CLEC4E Antibody (16E3) [Alexa Fluor® 488] [NBP1-49311AF488] - An intracellular stain was performed on U87MG cells with CLEC4E Antibody (16E3) NBP1-49311AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Applications for CLEC4E Antibody (16E3) [Alexa Fluor® 488]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-49311AF488 in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CLEC4E

CLEC4E encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein is a downstream target of CCAAT/enhancer binding protein (C/EBP), beta (CEBPB) and may play a role in inflammation. Alternative splice variants have been described but their full-length sequence has not been determined. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region.

Long Name

C-type Lectin Domain Family 4, Member E

Alternate Names

CLECSF9, MINCLE

Gene Symbol

CLEC4E

Additional CLEC4E Products

Product Documents for CLEC4E Antibody (16E3) [Alexa Fluor® 488]

Certificate of Analysis

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Product Specific Notices for CLEC4E Antibody (16E3) [Alexa Fluor® 488]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

C-type Lectin Family

Citations for CLEC4E Antibody (16E3) [Alexa Fluor® 488]

Customer Reviews for CLEC4E Antibody (16E3) [Alexa Fluor® 488] (1)

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  • CLEC4E Antibody (16E3) [Alexa Fluor® 488]
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 02/27/2019
    activated pbmcs
    CLEC4E Antibody (16E3) [Alexa Fluor® 488] NBP1-49311AF488

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