Cloudz Screening Kit

Dissolvable microspheres bound to streptavidin for flexible cell expansion options with any biotinylated ligand
Catalog # Availability Size / Price Qty
CLD007
Screening Cloudz composed of hydrogel-based dissolvable microspheres bound to Streptavidin
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Cloudz Screening Kit Summary

Flexible platform to screen ligands for identification of optimal cell expansion process. Cloudz can be conjugated to any biotinylated ligand for activation of cells. After cells are activated, our release buffer completely dissolves the Cloudz microspheres leaving behind a highly pure, expanded cell population. Don't see your preferred biotinlyated ligand? Our Novus team can biotinylate any antibody of interest via the custom team.

Key Benefits

Dissolvable hydrogels composed of alginate are cell friendly and easy to remove after activation
• Streptavidin-conjugated cloudz for use with any biotinylated ligand
• Cloudz microspheres dissolve in seconds after activation
• Customizable platform to tune to your specific process

Kit Contents

7 mL vial of Screening Cloudz (SPC-4276)
• 100 mL bottle of Cloudz 6X Release Buffer (SPC-4185)
• 100 mL Bottle of 1X Wash Buffer (SPC-4287)

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the recommended temperature.

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Data Example

Example of optimizing T cell activation with Screening Cloudz. Blends of Screening Cloudz were compared for activation in example CD3+ cell expansion protocol. A 1:1 blend of CD3 Screening Cloudz to CD28 Screening Cloudz demonstrated highest activation. Directly conjugated Custom Cloudz with 1:3 ratio of CD3 to CD28 demonstrated similar T cell activation to comparable 1:3 Screening Cloudz blend.

Example of optimizing T cell Expansion with Screening Cloudz. Screening Cloudz were functionalized with CD3 or CD28 antibody and blended together at various ratios. Blends of Screening Cloudz were compared expansion in example CD3+ cell expansion protocol. A 1:1 blend of CD3 Screening Cloudz to CD28 Screening Cloudz demonstrated highest expansion. Directly conjugated Custom Cloudz with 1:3 ratio of CD3 to CD28 demonstrated similar T cell expansion to comparable 1:3 Screening Cloudz blend.

Flow Cytometry View Larger

Example of T cell Activation with Screening Cloudz and similar Custom Cloudz Screening Cloudz (Cat # CLD007) (A) and Custom Cloudz (B) were functionalized with identical combinations of CD3 and CD28 antibodies. Primary human CD3+ cells were cultured with each set of Cloudz for 48 hours in ExCellerate T cell Expansion Media (CCM030) and 20 ng/mL IL-2 (Cat # 202-IL). The negative control was performed without adding the Cloudz. Cells were fluorescently stained for CD25 and CD69 and analyzed by flow cytometry. Dead cells and debris were excluded from the analysis based on scatter signals and viability dye.

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