Clozapine N-oxide

Catalog # Availability Size / Price Qty
4936/10
4936/50
DREADD Activators Small Molecule
1 Image
Description: Activator of muscarinic DREADDs

Chemical Name: 8-Chloro-11-(4-methyl-4-oxido-1-piperazinyl)-5H-dibenzo[b,e][1,4]diazepine

Purity: ≥99%

Product Details
Citations (31)
Supplemental Products
Reviews (7)

Biological Activity

Metabolite of clozapine (Cat. No. 0444). Activates human muscarinic designer receptors (DREADDs) in vitro. Silences hippocampal neurons expressing hM4 DREADDs in vitro. Activates Gs-linked DREADDS- AgRP in mice via intracerebroventricular (i.c.v) injection. Shown to be a P-glycoprotein (P-gp) efflux pump substrate. Other potent and selective hM3Dq DREADD agonists available: DREADD agonist 21 and Perlapine.

Water soluble salt also available.

This product contains 1 molar equivalent of ethanol.

Technical Data

M.Wt:
342.82
Formula:
C18H19ClN4O
Solubility:
Soluble to 20 mM in DMSO
Purity:
≥99%
Storage:
Store at RT
CAS No:
34233-69-7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets

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Citations for Clozapine N-oxide

The citations listed below are publications that use Tocris products. Selected citations for Clozapine N-oxide include:

31 Citations: Showing 1 - 10

  1. Prefrontal Theta Oscillations Promote Selective Encoding of Behaviorally Relevant Events.
    Authors: Jarovi Et al.
    Eneuro  2019;5
  2. Maladaptive Downregulation of Autonomous Subthalamic Nucleus Activity following the Loss of Midbrain Dopamine Neurons
    Authors: McIver Et al.
    Cell Rep  2019;28:992
  3. Activation of Astrocytic μ-Opioid Receptor Causes Conditioned Place Preference
    Authors: Nam Et al.
    Cell Rep  2019;28:1154
  4. Adenosine triphosphate is co-secreted with glucagon-like peptide-1 to modulate intestinal enterocytes and afferent neurons.
    Authors: Lu Et al.
    Nat.commun.  2019;10:1029
  5. Anterior cingulate cortex modulates the affective-motivative dimension of hyperosmolality-induced thirst.
    Authors: Ma Et al.
    J Physiol  2019;
  6. A hippocampal circuit linking dorsal CA2 to ventral CA1 critical for social memory dynamics.
    Authors: Meira Et al.
    Nat.Commun.  2018;9:4163
  7. The role of calretinin-expressing granule cells in olfactory bulb functions and odor behavior.
    Authors: Hardy Et al.
    Sci Rep  2018;8:9385
  8. Dynamic interaction of local and transhemispheric networks is necessary for progressive intensification of hippocampal seizures.
    Authors: Berglind Et al.
    Sci Rep  2018;8:5669
  9. Cholinergic neurons in medial septum maintain anxiety-like behaviors induced by chronic inflammatory pain.
    Authors: Jiang Et al.
    Neurosci Letts  2018;671:7
  10. Parallel Arousal Pathways in the Lateral Hypothalamus.
    Authors: Heiss Et al.
    Eneuro  2018;5
  11. Kisspeptin neurones in the posterodorsal medial amygdala modulate sexual partner preference and anxiety in male mice.
    Authors: Adekunbi Et al.
    J Neuroendocrinol  2018;30:e12572
  12. Excitatory Pathways from the Lateral Habenula Enable Propofol-Induced Sedation.
    Authors: Gelegen Et al.
    Curr Biol  2018;28:580
  13. A Neuronal Hub Binding Sleep Initiation and Body Cooling in Response to a Warm External Stimulus.
    Authors: Harding Et al.
    Curr Biol  2018;28:2263
  14. The Basolateral Amygdala Is Essential for Rapid Escape: A Human and Rodent Study.
    Authors: Terburg Et al.
    Cell  2018;175:723
  15. Neural Circuit-Specialized Astrocytes: Transcriptomic, Proteomic, Morphological, and Functional Evidence.
    Authors: Chai Et al.
    Neuron  2017;95:531
  16. A Brainstem-Spinal Cord Inhibitory Circuit for Mechanical Pain Modulation by GABA and Enkephalins.
    Authors: Francois Et al.
    Neuron  2017;93:822
  17. Glutamatergic projections from the entorhinal cortex to dorsal dentate gyrus mediate context-induced reinstatement of heroin seeking.
    Authors: Ge Et al.
    Neuropsychopharmacology  2017;42:1860
  18. Loss of Plasticity in the D2-Accumbens Pallidal Pathway Promotes Cocaine Seeking.
    Authors: Heinsbroek Et al.
    J Neurosci  2017;37:757
  19. A tale of two circuits: CCKNTS neuron stimulation controls appetite and induces opposing motivational states by projections to distinct brain regions.
    Authors: Roman Et al.
    Neuroscience  2017;358:316
  20. Cortico-accumbens regulation of approach-avoidance behavior Is modified by experience and chronic pain.
    Authors: Schwartz Et al.
    Cell Rep  2017;19:1522
  21. Inhibiting medial septal cholinergic neurons with DREADD alleviated anxiety-like behaviors in mice.
    Authors: Zhang Et al.
    Neurosci Letts  2017;18:139
  22. Visuomotor coupling shapes the functional development of mouse visual cortex.
    Authors: Attinger
    Cell  2017;169(7):1291
  23. Ethanol-seeking behavior is expressed directly through an extended amygdala to midbrain neural circuit.
    Authors: Pina and Cunningham Et al.
    Neurobiol Learn Mem  2017;137:83
  24. Using c-kit to genetically target cerebellar molecular layer interneurons in adult mice.
    Authors: Amat
    PLoS One  2017;12(6):e0179347
  25. Histamine modulation of the basal ganglia circuitry in the development of pathological grooming.
    Authors: Rapanelli
    Proc Natl Acad Sci U S A.  2017;114(25):6599
  26. Appetite controlled by a cholecystokinin nucleus of the solitary tract to hypothalamus neurocircuit.
    Authors: D'Agostino Et al.
    PLoS Biol  2016;5
  27. Attenuation of β-Amyloid Deposition and Neurotoxicity by Chemogenetic Modulation of Neural Activity.
    Authors: Yuan and Grutzendler
    J Neurosci  2016;36:632
  28. Neural activity promotes long-distance, target-specific regeneration of adult retinal axons.
    Authors: Lim Et al.
    Nat Neurosci  2016;19:1073
  29. Warm-Sensitive Neurons that Control Body Temperature.
    Authors: Tan Et al.
    Cell  2016;167:47
  30. Ethanol-seeking behavior is expressed directly through an extended amygdala to midbrain neural circuit.
    Authors: Pina and Cunningham
    Neurobiol Learn Mem  2016;137:83
  31. DREADDS: Use and application in behavioral neuroscience.
    Authors: Smith Et al.
    Behav Neurosci  2016;130:137

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Reviews for Clozapine N-oxide

Average Rating: 4.6 (Based on 7 Reviews)

5 Star
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4 Star
42.86%
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Highly soluble and stable
By Anonymous on 01/04/2018
Species: Mouse

Soluble in 0.9% saline. Perfused in brain slice experiment with patch clamp study.


CNO in DREADD pain targeted Mice
By Anonymous on 12/15/2017
Species: Mouse

CNO was used in pair with DREADD mice for a pain related experiment to determine whether inhibiting PBN neurons will attenuate pain reponses. Publication under second revision for Cell.

Product provides publishable work, no issues arose when dealing with the substance.


CNO in concert with DREADDs
By Anonymous on 12/01/2017
Species: Rat

I injected clozapine N-oxide in rats (IP, 1 mg/ml/kg). The rats had previously underwent stereotaxic surgery in which a virus carrying a gene for a Gi DREADD was infused unilaterally into the dorsal striatum.


CNO for chronic DREADD activation
By Anonymous on 12/01/2017
Species: Mouse

In vivo chemogenetic experiments - CNO was added to the drinking water. While there are issues with the back conversion of CNO to clozapine, this is not the manufacturer's problem. CNO experiments still generate important data as long as they utilize proper controls

Added saccharine to counteract bitter taste of DMSO used to dissolve CNO


Does what it is meant to!
By Anonymous on 10/20/2017
Species: Rat

We use it as both injectable pass the blood brain barrier ICV, and IP injections. We are looking at both behavioral concern and brain regional efficacy in regard to DREADD. So far, this product has been selectively turning on/off our target cells.


tail bleed
By Anonymous on 09/07/2017
Species: Mouse

bleed assay


neuron silencer
By Anonymous on 07/17/2017
Species: Mouse

neuron silencer


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