Clozapine N-oxide

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DREADD Activators Small Molecule
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Description: Activator of muscarinic DREADDs

Chemical Name: 8-Chloro-11-(4-methyl-4-oxido-1-piperazinyl)-5H-dibenzo[b,e][1,4]diazepine

Purity: ≥99%

Product Details
Citations (14)
Supplemental Products
Reviews (7)

Biological Activity

Metabolite of clozapine (Cat. No. 0444). Activates human muscarinic designer receptors (DREADDs) in vitro. Silences hippocampal neurons expressing hM4 DREADDs in vitro. Activates Gs-linked DREADDS- AgRP in mice via intracerebroventricular (i.c.v) injection. Shown to be a P-glycoprotein (P-gp) efflux pump substrate. Other potent and selective hM3Dq DREADD agonists available: DREADD agonist 21 and Perlapine.

Water soluble salt also available.

This product contains 1 molar equivalent of ethanol.

Technical Data

Soluble to 20 mM in DMSO
Store at RT

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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Citations for Clozapine N-oxide

The citations listed below are publications that use Tocris products. Selected citations for Clozapine N-oxide include:

14 Citations: Showing 1 - 10

  1. A hippocampal circuit linking dorsal CA2 to ventral CA1 critical for social memory dynamics.
    Authors: Meira Et al.
    Nat.Commun.  2018;9:4163
  2. Cholinergic neurons in medial septum maintain anxiety-like behaviors induced by chronic inflammatory pain.
    Authors: Jiang Et al.
    Neurosci Letts  2018;671:7
  3. Kisspeptin neurones in the posterodorsal medial amygdala modulate sexual partner preference and anxiety in male mice.
    Authors: Adekunbi Et al.
    J Neuroendocrinol  2018;30:e12572
  4. Glutamatergic projections from the entorhinal cortex to dorsal dentate gyrus mediate context-induced reinstatement of heroin seeking.
    Authors: Ge Et al.
    Neuropsychopharmacology  2017;42:1860
  5. A tale of two circuits: CCKNTS neuron stimulation controls appetite and induces opposing motivational states by projections to distinct brain regions.
    Authors: Roman Et al.
    Neuroscience  2017;358:316
  6. Cortico-accumbens regulation of approach-avoidance behavior Is modified by experience and chronic pain.
    Authors: Schwartz Et al.
    Cell Rep  2017;19:1522
  7. Inhibiting medial septal cholinergic neurons with DREADD alleviated anxiety-like behaviors in mice.
    Authors: Zhang Et al.
    Neurosci Letts  2017;18:139
  8. Visuomotor coupling shapes the functional development of mouse visual cortex.
    Authors: Attinger
    Cell  2017;169(7):1291
  9. Ethanol-seeking behavior is expressed directly through an extended amygdala to midbrain neural circuit.
    Authors: Pina and Cunningham Et al.
    Neurobiol Learn Mem  2017;137:83
  10. Using c-kit to genetically target cerebellar molecular layer interneurons in adult mice.
    Authors: Amat
    PLoS One  2017;12(6):e0179347


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Reviews for Clozapine N-oxide

Average Rating: 4.6 (Based on 7 Reviews)

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Highly soluble and stable
By Anonymous on 01/04/2018
Species: Mouse

Soluble in 0.9% saline. Perfused in brain slice experiment with patch clamp study.

CNO in DREADD pain targeted Mice
By Anonymous on 12/15/2017
Species: Mouse

CNO was used in pair with DREADD mice for a pain related experiment to determine whether inhibiting PBN neurons will attenuate pain reponses. Publication under second revision for Cell.

Product provides publishable work, no issues arose when dealing with the substance.

CNO in concert with DREADDs
By Anonymous on 12/01/2017
Species: Rat

I injected clozapine N-oxide in rats (IP, 1 mg/ml/kg). The rats had previously underwent stereotaxic surgery in which a virus carrying a gene for a Gi DREADD was infused unilaterally into the dorsal striatum.

CNO for chronic DREADD activation
By Anonymous on 12/01/2017
Species: Mouse

In vivo chemogenetic experiments - CNO was added to the drinking water. While there are issues with the back conversion of CNO to clozapine, this is not the manufacturer's problem. CNO experiments still generate important data as long as they utilize proper controls

Added saccharine to counteract bitter taste of DMSO used to dissolve CNO

Does what it is meant to!
By Anonymous on 10/20/2017
Species: Rat

We use it as both injectable pass the blood brain barrier ICV, and IP injections. We are looking at both behavioral concern and brain regional efficacy in regard to DREADD. So far, this product has been selectively turning on/off our target cells.

tail bleed
By Anonymous on 09/07/2017
Species: Mouse

bleed assay

neuron silencer
By Anonymous on 07/17/2017
Species: Mouse

neuron silencer

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