Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Amphibian, Avian, Mammal, Sheep

Cited:

Human, Mouse, Rat, Avian, Canine, Ovine

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Equine (100%), Feline (100%), Finch (100%), Goat (100%), Guinea Pig (100%), Hamster (100%), Primate (100%), Rabbit (100%), Vole (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Collagen I alpha 1 Primary Antibodies »

Product Specifications

Immunogen

This Collagen I alpha 1 antibody was raised against synthetic peptide corresponding to amino acid residues within the C-terminal telo peptide portion (aa1193-1218) of the human COL1A1, conjugated to KLH. Accession # P02452

Reactivity Notes

Based on the homology of the immunogen, this antibody is expected to recognize the collagen I alpha1 polypeptide in all mammals, birds, and amphibians.

Specificity

This Collagen I alpha 1 antibody is specific for the ~ 140 kDa telopeptide portion of the collagen I alpha 1 polypeptide. The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 degrees C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehydefixed fibrotic mouse lung tissue, the antibody recognizes mature collagen I that has formed fibrils in the extracellular matrix.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for Collagen I alpha 1 Antibody - Azide and BSA Free

Western Blot: Collagen I alpha 1 AntibodyAzide and BSA Free [NBP1-30054]

Western Blot: Collagen I alpha 1 AntibodyAzide and BSA Free [NBP1-30054]

Western Blot: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Rat lung lysate showing specific immunolabeling of the collagen protein with an observed molecular weight ~140.
Immunohistochemistry: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054]

Immunohistochemistry: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054]

Collagen-I-alpha-1-Antibody---Azide-and-BSA-Free-Immunohistochemistry-NBP1-30054-img0006.jpg
Immunohistochemistry: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054]

Immunohistochemistry: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054]

Immunohistochemistry: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (red) that has formed fibrils in the extracellular matrix.
Simple Western: Collagen I alpha 1 AntibodyAzide and BSA Free [NBP1-30054]

Simple Western: Collagen I alpha 1 AntibodyAzide and BSA Free [NBP1-30054]

Simple Western: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Simple Western lane view shows a specific band for Collagen I Alpha 1 in 0.5 mg/ml of Human Lung (left) and Human Kidney (right) lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody
Collagen I alpha 1 Antibody - Azide and BSA Free

Western Blot: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] -

Western Blot: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - TNC deficiency reduced kidney fibrosis in animal models.Deletion of TNC (TNC−/−) significantly attenuated the induction of collagen I assessed by IF following UUO by approximate 30% at day 7 & 10 compared with their wild-type littermates (A, n = 4 for each time point, two-way ANOVA p < 0.05). Consistently, the expression of fibrosis markers, such as collagen I alpha, fibronectin & plasminogen activator inhibitor-1 (PAI-1), were significantly lower in TNC−/− mice at UUO day 7 & 10 (B, n = 4 for each time point, two-way ANOVA, p < 0.05). Western blot showed that the proteins of collagen I alpha & alpha -SMA were also significantly reduced in TNC−/− mice at UUO day 7 (C, n = 7, p < 0.05). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/36522320), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Collagen I alpha 1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] -

Pathological staining analysis of 28-day animal experiment induced by BAPN. (A), representative images from elastic van Gieson (EVG) staining, along with statistical analysis of elastin degradation. (B), representative images from hematoxylin and eosin (HE) staining are presented, accompanied by statistical analysis of aortic wall thickness. The scale bar for both panels (A) and (B) is 200 μm. (C), immunofluorescence staining of Col1a1, accompanied by quantitative analysis of the Col1a1 positive area in the vascular wall, scale bar 50 μm Image collected and cropped by CiteAb from the following open publication (https://nutritionandmetabolism.biomedcentral.com/articles/10.1186/s1298…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Collagen I alpha 1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] -

Pathological staining analysis of 28-day animal experiment induced by BAPN. (A), representative images from elastic van Gieson (EVG) staining, along with statistical analysis of elastin degradation. (B), representative images from hematoxylin and eosin (HE) staining are presented, accompanied by statistical analysis of aortic wall thickness. The scale bar for both panels (A) and (B) is 200 μm. (C), immunofluorescence staining of Col1a1, accompanied by quantitative analysis of the Col1a1 positive area in the vascular wall, scale bar 50 μm Image collected and cropped by CiteAb from the following open publication (https://nutritionandmetabolism.biomedcentral.com/articles/10.1186/s1298…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Collagen I alpha 1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:100

Simple Western

1:100

Western Blot

1:1000
Application Notes
Use in Immunohistochemistry-Frozen sections was reported in the scientific literature (PMID: 21939397), IHC reactivity reported in scientific literature (PMID: 26045736). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 26651081).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Human Lung and Human Kidney lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:100. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 2 reviews rated 4 using NBP1-30054 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Collagen I alpha 1

Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Collagens are highly conserved and are characterized by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. The extensive family of collagens is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagen type I (also known as collagen alpha, COL1A1, and alpha-1 type I collagen) is the largest component of fibrillar collagen found in cartilage and connective tissues. It is synthesized by fibroblasts, osteoblasts, and odontoblasts and has a theoretical molecular weight of 138 kDa.

Type I collagen is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon tissue. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue. Several collagens play a role in cell adhesion, responsible for maintaining normal tissue architecture and function. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Post-Golgi LH3 trafficking is essential for collagen homeostasis and for the development and function of multiple organs and tissues (1).

The COL1A1 gene encodes the pro-alpha1 chains of type I collagen protein, whose triple helix is comprised of two alpha1 chains and one alpha2 chain. Mutations in the encoding COL1A1 gene are associated with brittle bone disease (Osteogenesis Imperfecta), cortical hyperostosis (Caffey disease) and disorders that affect the connective tissues (Ehlers-Danlos syndrome) (2). Studies have found that HIF-1 transcription regulation of collagen prolyl hydroxylases regulates collagen deposition, promoting cancer cell alignment along collagen fibers, which enhances invasion and metastasis to lymph nodes and lung tissue by breast cancer cells (3).

References

1. Banushi, B., Forneris, F., Straatman-Iwanowska, A., Strange, A., Lyne, A. M., Rogerson, C.,... Gissen, P. (2016). Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis. Nat Commun, 7, 12111. doi:10.1038/ncomms12111

2. Lu, Y., Zhang, S., Wang, Y., Ren, X., & Han, J. (2019). Molecular mechanisms and clinical manifestations of rare genetic disorders associated with type I collagen. Intractable Rare Dis Res, 8(2), 98-107. doi:10.5582/irdr.2019.01064

3. Gilkes, D. M., Chaturvedi, P., Bajpai, S., Wong, C. C., Wei, H., Pitcairn, S.,... Semenza, G. L. (2013). Collagen prolyl hydroxylases are essential for breast cancer metastasis. Cancer Res, 73(11), 3285-3296. doi:10.1158/0008-5472.Can-12-3963

Alternate Names

COL1A1, OI4

Entrez Gene IDs

1277 (Human); 12842 (Mouse); 29393 (Rat)

Gene Symbol

COL1A1

UniProt

P02452

Additional Collagen I alpha 1 Products

Product Documents for Collagen I alpha 1 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Collagen I alpha 1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Collagen I alpha 1 Antibody - Azide and BSA Free

Customer Reviews for Collagen I alpha 1 Antibody - Azide and BSA Free (2)

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  • Collagen I alpha 1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse Pancreas
    Species: Mouse
    Verified Customer | Posted 10/02/2020
    Caerulein dosed mouse pancreas
    Collagen I alpha 1 Antibody - Azide and BSA Free NBP1-30054
  • Collagen I alpha 1 Antibody
    Name: wang xuyang
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Adult heart
    Species: Rat
    Verified Customer | Posted 12/20/2017
    collagen I for sd rat‘s heart
    Collagen I alpha 1 Antibody - Azide and BSA Free NBP1-30054

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Collagen I alpha 1 Antibody - Azide and BSA Free

Showing  1 - 5 of 7 FAQs Showing All

  • Q: Do you have any references on IHC-P performed on sheep tissue?

    A: Unfortunately we do not have any references of IHC-P performed on sheep tissue. However, I can confirm that this has been tested and will be backed by our 100% guarantee. Therefore if the antibody fails to stain sheep tissue that expresses the protein in an IHC-P assay, Novus would be happy to issue a refund or replacement.

  • Q: How long does delivery take for your collagen 1 alpha 1 antibody?

    A: Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.

  • Q: I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?

    A: Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).

  • Q: I intend buying anti-collagen I antibody for IF and WB. Kindly let me know its isotype

    A: NBP1-30054 is a rabbit IgG polyclonal antibody.

  • Q: The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?

    A: Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.

  • Q: We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?

    A:

    We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).

  • Q: What secondary antibody do you recommend for collagen I alpha 1 antibodies?

    A: Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.

  • Q: Do you have any references on IHC-P performed on sheep tissue?

    A: Unfortunately we do not have any references of IHC-P performed on sheep tissue. However, I can confirm that this has been tested and will be backed by our 100% guarantee. Therefore if the antibody fails to stain sheep tissue that expresses the protein in an IHC-P assay, Novus would be happy to issue a refund or replacement.

  • Q: How long does delivery take for your collagen 1 alpha 1 antibody?

    A: Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.

  • Q: I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?

    A: Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).

  • Q: I intend buying anti-collagen I antibody for IF and WB. Kindly let me know its isotype

    A: NBP1-30054 is a rabbit IgG polyclonal antibody.

  • Q: The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?

    A: Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.

  • Q: We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?

    A:

    We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).

  • Q: What secondary antibody do you recommend for collagen I alpha 1 antibodies?

    A: Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.

  • Q: Do you have any references on IHC-P performed on sheep tissue?

    A: Unfortunately we do not have any references of IHC-P performed on sheep tissue. However, I can confirm that this has been tested and will be backed by our 100% guarantee. Therefore if the antibody fails to stain sheep tissue that expresses the protein in an IHC-P assay, Novus would be happy to issue a refund or replacement.

  • Q: How long does delivery take for your collagen 1 alpha 1 antibody?

    A: Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.

  • Q: I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?

    A: Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).

  • Q: I intend buying anti-collagen I antibody for IF and WB. Kindly let me know its isotype

    A: NBP1-30054 is a rabbit IgG polyclonal antibody.

  • Q: The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?

    A: Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.

  • Q: We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?

    A:

    We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).

  • Q: What secondary antibody do you recommend for collagen I alpha 1 antibodies?

    A: Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.

  • Q: Do you have any references on IHC-P performed on sheep tissue?

    A: Unfortunately we do not have any references of IHC-P performed on sheep tissue. However, I can confirm that this has been tested and will be backed by our 100% guarantee. Therefore if the antibody fails to stain sheep tissue that expresses the protein in an IHC-P assay, Novus would be happy to issue a refund or replacement.

  • Q: How long does delivery take for your collagen 1 alpha 1 antibody?

    A: Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.

  • Q: I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?

    A: Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).

  • Q: I intend buying anti-collagen I antibody for IF and WB. Kindly let me know its isotype

    A: NBP1-30054 is a rabbit IgG polyclonal antibody.

  • Q: The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?

    A: Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.

  • Q: We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?

    A:

    We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).

  • Q: What secondary antibody do you recommend for collagen I alpha 1 antibodies?

    A: Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.

  • Q: Do you have any references on IHC-P performed on sheep tissue?

    A: Unfortunately we do not have any references of IHC-P performed on sheep tissue. However, I can confirm that this has been tested and will be backed by our 100% guarantee. Therefore if the antibody fails to stain sheep tissue that expresses the protein in an IHC-P assay, Novus would be happy to issue a refund or replacement.

  • Q: How long does delivery take for your collagen 1 alpha 1 antibody?

    A: Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.

  • Q: I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?

    A: Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).

  • Q: I intend buying anti-collagen I antibody for IF and WB. Kindly let me know its isotype

    A: NBP1-30054 is a rabbit IgG polyclonal antibody.

  • Q: The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?

    A: Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.

  • Q: We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?

    A:

    We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).

  • Q: What secondary antibody do you recommend for collagen I alpha 1 antibodies?

    A: Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.

  • Q: Do you have any references on IHC-P performed on sheep tissue?

    A: Unfortunately we do not have any references of IHC-P performed on sheep tissue. However, I can confirm that this has been tested and will be backed by our 100% guarantee. Therefore if the antibody fails to stain sheep tissue that expresses the protein in an IHC-P assay, Novus would be happy to issue a refund or replacement.

  • Q: How long does delivery take for your collagen 1 alpha 1 antibody?

    A: Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.

  • Q: I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?

    A: Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).

  • Q: I intend buying anti-collagen I antibody for IF and WB. Kindly let me know its isotype

    A: NBP1-30054 is a rabbit IgG polyclonal antibody.

  • Q: The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?

    A: Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.

  • Q: We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?

    A:

    We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).

  • Q: What secondary antibody do you recommend for collagen I alpha 1 antibodies?

    A: Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.

  • Q: Do you have any references on IHC-P performed on sheep tissue?

    A: Unfortunately we do not have any references of IHC-P performed on sheep tissue. However, I can confirm that this has been tested and will be backed by our 100% guarantee. Therefore if the antibody fails to stain sheep tissue that expresses the protein in an IHC-P assay, Novus would be happy to issue a refund or replacement.

  • Q: How long does delivery take for your collagen 1 alpha 1 antibody?

    A: Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.

  • Q: I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?

    A: Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).

  • Q: I intend buying anti-collagen I antibody for IF and WB. Kindly let me know its isotype

    A: NBP1-30054 is a rabbit IgG polyclonal antibody.

  • Q: The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?

    A: Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.

  • Q: We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?

    A:

    We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).

  • Q: What secondary antibody do you recommend for collagen I alpha 1 antibodies?

    A: Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.

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