Collagen II Antibody [FITC]

Novus Biologicals | Catalog # NBP2-46876

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Sheep

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG
View all Collagen II Primary Antibodies »

Product Specifications

Immunogen

Collagen II from human knee cartilage and bovine nasal cartilage. (Uniprot: P02458)

Reactivity Notes

This antibody reacts with most mammalian Collagen II and has expected cross-reactivity with Type IV and VI and negligible cross reactivity to Type I, III and V collagens.

Canine reactivity reported in scientific literature (PMID: 22972852).

Avian reactivity reported in scientific literature (PMID: 24739280).

Bovine reactivity reported in scientific literature (PMID: 23688110).

Silk worm reactivity reported in scientific literature (PMID: 23845228).

Specificity

Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Collagen II and has expected cross-reactivity with Type IV and VI and negligible cross reactivity to Type I, III and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities

Scientific Data Images for Collagen II Antibody [FITC]

Immunohistochemistry: Collagen II Antibody [Fluorescein] [NBP2-46876] - Analysis of Tissue: human bronchiolar cartilage (shown). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining. Fixation: formalin fixed paraffin embedded. Antigen retrieval: 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes. Primary antibody: collagen II antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen II is extracellular. Staining: Collagen II as precipitated red signal with hematoxylin purple nuclear counterstain.

Applications for Collagen II Antibody [FITC]

Application
Recommended Usage

ELISA

1:10000 - 1:50000

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:200 - 1:1000

Immunoprecipitation

1:100

Western Blot

1:5000-1:10000
Application Notes
This product is suitable for western blotting, IP, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
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Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

Reconstitute with 50 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Collagen II

The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function. In cartilage, Collagen Type II constitutes the bulk of the fibril. Sensitization with Collagen Type II induces an erosive polyarthritis in rats, mice and higher primates which can resemble rheumatoid arthritis and relapsing polychrondritis.

Alternate Names

Chondrocalcin, COL2A1, SEDC

Entrez Gene IDs

1280 (Human); 407142 (Bovine)

Gene Symbol

COL2A1

UniProt

P02458 (Human)

Additional Collagen II Products

Product Documents for Collagen II Antibody [FITC]

Certificate of Analysis

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Product Specific Notices for Collagen II Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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